cDNA Clone HEPS HEPt

• Plasmid: pETDuet Stearmothermophillus HEPS 6His HEPT
• Parent Plasmid: pETDuet1
• Genbank: P55785 and P55784
• Tag: 6His
• Species: Geobacillus stearophilus

Sequence of Insert

DNA sequence HEPS insert :
CATATGCTCGATGGCGCTTCAACGGCGCCGAGTGAGGCGGAGCGGTGCAT
CATCGCCATGATGCTCATGCAGATCGCCCTTGATACCCACGATGAGGTGA
CAGATGACGGCGGCGACTTGCGGGCGCGGCAGCTTGTCGTCCTGGCCGGC
GACTTGTACAGCGGGCTGTACTATGAGTTGTTGGCGCGTTCGGGCGAAAC
GGCGCTCATCCGCTCGTTCGCCGAGGCGGTCCGCGATATTAACGAGCAAA
AAGTGCGGCTTTACGAAAAAAAAGTAGAGCGGATCGAGTCGTTGTTTGCG
GCGGTCGGCACGATCGAATCGGCGTTGCTTGTCAAGCTCGCCGACCGCAT
GGCGGCGCCGCAGTGGGGGCAGTTTGCCTATTCGTATTTGCTGATGCGGC
GCCTGCTGCTCGAGCAGGAAGCGTTCATCCGCACGGGAGCTTCGGTGCTC
TTTGAGCAAATGGCGCAAATCGCGTTCCCGCGCGCGAAAACGTTGACGAA
AGAGCAAAAGCGGCATTTGCTCCGCTTTTGCCGCCGCTATATCGACGGCT
GCCGGGAGGCGCTGTTTGCGGCGAAACTGCCGGTCAACGGCCTGCTGCAG
CTCCGCGTGGCCGAGCTTTCCGGCGGGTTTCAAGCCATCGCCAAAAAGAC
GGTGGAAGAAGGGTAGGGTACC

DNA sequence HEPT insert : GAATTCGATGAAGTTAAAGGCGATGTATTCGTTTTTAAGCGATGATTTAG
CGGCGGTCGAAGAGGAGCTTGAGCGGGCGGTTCAGTCGGAATACGGGCCG
CTTGGGGAAGCGGCGCTCCATCTGTTGCAGGCGGGCGGAAAGCGGATCCG
TCCCGTTTTTGTCTTGCTTGCCGCCCGCTTCGGCCAATATGACCTTGAGC
GGATGAAGCATGTTGCCGTTGCGCTCGAGCTCATTCATATGGCTTCGCTC
GTCCACGACGATGTGATCGACGACGCCGATTTGCGCCGCGGCCGGCCGAC
GATCAAGGCGAAATGGAGCAACCGGTTCGCCATGTACACAGGGGATTATT
TGTTTGCCCGCTCGCTCGAACGGATGGCGGAGCTCGGCAACCCGCGCGCC
CATCAAGTGTTGGCGAAAACGATCGTGGAAGTGTGCCGCGGGGAAATTGA
GCAAATTAAAGACAAGTACCGGTTTGATCAGCCGCTGCGCACGTATTTGC
GGCGCATCCGTCGGAAAACGGCGCTGCTCATCGCCGCGAGCTGCCAGCTT
GGCGCCCTCGCTGCCGGCGCGCCGGAGCCGATTGTGAAGCGGCTGTACTG
GTTCGGCCATTATGTCGGCATGTCGTTTCAAATTACCGACGACATTCTCG
ATTTCACTGGGACGGAGGAACAGCTCGGCAAACCGGCCGGAAGCGACTTG
CTACAAGGAAACGTCACCCTTCCTGTGCTGTATGCCTTGAGCGATGAGCG
GGTGAAGGCGGCCATTGCAGCTGTCGGTCCGGAAACGGACGTTGCGGAAA
TGGCGGCGGTCATTTCCGCCATTAAGCGGACGGACGCCATTGAGCGGTCG
TATGCGTTAAGCGACCGTTACCTTGACAAGGCGCTTCACCTTCTTGACGG
ACTGCCGATGAATGAGGCGCGCGGCCTGTTGCGCGACCTCGCCCTTTACA
TCGGGAAAAGGGATTATTAAGCGGCCGC

Amino Acid Sequence

Protein sequence of HEPS :
MLDGASTAPSEAERCIIAMMLMQIALDTHDEVTDDGGDLRARQLVVLAGD
LYSGLYYELLARSGETALIRSFAEAVRDINEQKVRLYEKKVERIESLFAA
VGTIESALLVKLADRMAAPQWGQFAYSYLLMRRLLLEQEAFIRTGASVLF
EQMAQIAFPRAKTLTKEQKRHLLRFCRRYIDGCREALFAAKLPVNGLLQL
RVAELSGGFQAIAKKTVEEG*

Protein sequence of HEPT : MGSHHHHHHQDPNSMKLKAMYSFLSDDLAAVEEELERAVQSEYGPLGEAA
LHLLQAGGKRIRPVFVLLAARFGQYDLERMKHVAVALELIHMASLVHDDV
IDDADLRRGRPTIKAKWSNRFAMYTGDYLFARSLERMAELGNPRAHQVLA
KTIVEVCRGEIEQIKDKYRFDQPLRTYLRRIRRKTALLIAASCQLGALAA
GAPEPIVKRLYWFGHYVGMSFQITDDILDFTGTEEQLGKPAGSDLLQGNV
TLPVLYALSDERVKAAIAAVGPETDVAEMAAVISAIKRTDAIERSYALSD
RYLDKALHLLDGLPMNEARGLLRDLALYIGKRDY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8875
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Dual expression vector for bacterial expression.; ; Estimated MW of expressed HEPS : 24633 Daltons; ; Estimated MW of expressed HEPT : 37445 Daltons