cDNA Clone ERK8

• Synonyms: MAPK15
• Plasmid: pFastbac GST ERK8
• Parent Plasmid: pFastbac GST
• Expressed: GST-ERK8
• Genbank: AY065978
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTGCACCGTAGTGGACCCTCGCATTGTCCGGAGATACCTACTCAG
GCGGCAGCTCGGGCAGGGGGCCTATGGCATTGTGTGGAAGGCAGTGGACC
GGAGGACTGGTGAGGTCGTGGCCATCAAGAAAATCTTTGATGCTTTTAGG
GATAAGACAGATGCCCAGAGAACATTCCGGGAAATCACGCTCCTCCAGGA
GTTTGGGGACCATCCCAACATCATCAGCCTCCTTGACGTGATCCGGGCAG
AGAACGACAGGGACATTTACCTGGTGTTTGAGTTTATGGACACTGACCTG
AACGCAGTCATCCGGAAGGGCGGCCTGCTGCAGGACGTCCACGTGCGCTC
CATCTTCTACCAGCTCCTGCGGGCCACCCGGTTCCTCCACTCGGGGCACG
TTGTGCACCGGGACCAGAAGCCGTCCAATGTGCTCCTGGATGCCAACTGC
ACAGTGAAGCTGTGTGACTTTGGCCTGGCCCGCTCCCTGGGCGACCTCCC
TGAGGGGCCTGAGGACCAGGCCGTGACAGAGTACGTGGCCACACGCTGGT
ACCGAGCACCGGAGGTGCTGCTCTCTTCGCACCGATACACCCTTGGGGTG
GACATGTGGAGTCTGGGCTGTATCCTGGGGGAGATGCTGCGGGGGAGACC
CCTGTTCCCCGGCACGTCCACCCTCCACCAGCTGGAGCTGATCCTGGAGA
CCATCCCACCGCCATCTGAGGAGGACCTCCTGGCTCTCGGCTCAGGCTGC
CGTGCCTCTGTGCTGCACCAGCTGGGGTCCCGGCCACGACAGACGCTGGA
TGCCCTCCTACCGCCAGACACCTCCCCAGAGGCCTTGGACCTCCTTAGGC
GACTCCTGGTGTTCGCCCCGGACAAGCGGTTAAGCGCGACCCAGGCACTG
CAGCACCCCTACGTGCAGAGGTTCCACTGCCCCAGCGACGAGTGGGCACG
AGAGGCAGATGTGCGGCCCCGGGCACACGAAGGGGTCCAGCTCTCTGTGC
CTGAGTACCGCAGCCGCGTCTATCAGATGATCCTGGAGTGTGGAGGCAGC
AGCGGCACCTCGAGAGAGAAGGGCCCGGAGGGTGTCTCCCCAAGCCAGGC
ACACCTGCACAAACCCAGAGCCGACCCTCAGCTGCCTTCTAGGACACCTG
TGCAGGGTCCCAGACCCAGGCCCCAGAGCAGCCCAGGCCATGACCCTGCC
GAGCACGAGTCCCCCCGTGCAGCCAAGAACGTTCCCAGGCAGAACTCCGC
TCCCCTGCTCCAAACTGCTCTCCTAGGGAATGGGGAAAGGCCCCCTGGGG
CGAAGGAAGCGCCCCCCTTGACACTCTCGCTGGTGAAGCCAAGCGGGAGG
GGAGCTGCGCCCTCCCTGACCTCCCAGGCTGCGGCTCAGGTGGCCAACCA
GGCCCTGATCCGGGGTGACTGGAACCGGGGCGGTGGGGTGAGGGTGGCCA
GCGTACAACAGGTCCCTCCCCGGCTTCCTCCGGAGGCCCGGCCCGGCCGG
AGGATGTTCAGCACCTCTGCCTTGCAGGGTGCCCAGGGGGGTGCCAGGGC
TTTGCTTGGAGGCTACTCCCAAGCCTACGGGACTGTCTGCCACTCGGCAC
TGGGCCACCTGCCCCTGCTGGAGGGGCACCATGTGTGAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSCTVVDPRIVRRYLLRRQLG
QGAYGIVWKAVDRRTGEVVAIKKIFDAFRDKTDAQRTFREITLLQEFGDH
PNIISLLDVIRAENDRDIYLVFEFMDTDLNAVIRKGGLLQDVHVRSIFYQ
LLRATRFLHSGHVVHRDQKPSNVLLDANCTVKLCDFGLARSLGDLPEGPE
DQAVTEYVATRWYRAPEVLLSSHRYTLGVDMWSLGCILGEMLRGRPLFPG
TSTLHQLELILETIPPPSEEDLLALGSGCRASVLHQLGSRPRQTLDALLP
PDTSPEALDLLRRLLVFAPDKRLSATQALQHPYVQRFHCPSDEWAREADV
RPRAHEGVQLSVPEYRSRVYQMILECGGSSGTSREKGPEGVSPSQAHLHK
PRADPQLPSRTPVQGPRPRPQSSPGHDPAEHESPRAAKNVPRQNSAPLLQ
TALLGNGERPPGAKEAPPLTLSLVKPSGRGAAPSLTSQAAAQVANQALIR
GDWNRGGGVRVASVQQVPPRLPPEARPGRRMFSTSALQGAQGGARALLGG
YSQAYGTVCHSALGHLPLLEGHHV*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8851
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 86521 Daltons