cDNA Clone SYK

• Plasmid: pFBHTb SYK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-SYK
• Genbank: AAH01645.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCCAGCAGCGGCATGGCTGACAGCGCCAACCACCTGCCCTT
CTTTTTCGGCAACATCACCCGGGAGGAGGCAGAAGATTACCTGGTCCAGG
GGGGCATGAGTGATGGGCTTTATTTGCTGCGCCAGAGCCGCAACTACCTG
GGTGGCTTCGCCCTGTCCGTGGCCCACGGGAGGAAGGCACACCACTACAC
CATCGAGCGGGAGCTGAATGGCACCTACGCCATCGCCGGTGGCAGGACCC
ATGCCAGCCCCGCCGACCTCTGCCACTACCACTCCCAGGAGTCTGATGGC
CTGGTCTGCCTCCTCAAGAAGCCCTTCAACCGGCCCCAAGGGGTGCAGCC
CAAGACTGGGCCCTTTGAGGATTTGAAGGAAAACCTCATCAGGGAATATG
TGAAGCAGACATGGAACCTGCAGGGTCAGGCTCTGGAGCAGGCCATCATC
AGTCAGAAGCCTCAGCTGGAGAAGCTGATCGCTACCACAGCCCATGAAAA
AATGCCTTGGTTCCATGGAAAAATCTCTCGGGAAGAATCTGAGCAAATTG
TCCTGATAGGATCAAAGACAAATGGAAAGTTCCTGATCCGAGCCAGAGAC
AACAACGGCTCCTACGCCCTGTGCCTGCTGCACGAAGGGAAGGTGCTGCA
CTATCGCATCGACAAAGACAAGACAGGGAAGCTCTCCATCCCCGAGGGAA
AGAAGTTCGACACGCTCTGGCAGCTAGTCGAGCATTATTCTTATAAAGCA
GATGGTTTGTTAAGAGTTCTTACTGTCCCATGTCAAAAAATCGGCACACA
GGGAAATGTTAATTTTGGAGGCCGTCCACAACTTCCAGGTTCCCATCCTG
CGACTTGGTCAGCGGGTGGAATAATCTCAAGAATCAAATCATACTCCTTC
CCAAAGCCTGGCCACAGAAAGTCCTCCCCTGCCCAAGGGAACCGGCAAGA
GAGTACTGTGTCATTCAATCCGTATGAGCCAGAACTTGCACCCTGGGCTG
CAGACAAAGGCCCCCAGAGAGAAGCCCTACCCATGGACACAGAGGTGTAC
GAGAGCCCCTACGCGGACCCTGAGGAGATCAGGCCCAAGGAGGTTTACCT
GGACCGAAAGCTGCTGACGCTGGAAGACAAAGAACTGGGCTCTGGTAATT
TTGGAACTGTGAAAAAGGGCTACTACCAAATGAAAAAAGTTGTGAAAACC
GTGGCTGTGAAAATACTGAAAAACGAGGCCAATGACCCCGCTCTTAAAGA
TGAGTTATTAGCAGAAGCAAATGTCATGCAGCAGCTGGACAACCCGTACA
TCGTGCGCATGATCGGGATATGCGAGGCCGAGTCCTGGATGCTAGTTATG
GAGATGGCAGAACTTGGTCCCCTCAATAAGTATTTGCAGCAGAACAGACA
TGTCAAGGATAAGAACATCATAGAACTGGTTCATCAGGTTTCCATGGGCA
TGAAGTACTTGGAGGAGAGCAATTTTGTGCACAGAGATCTGGCTGCAAGA
AATGTGTTGCTAGTTACCCAACATTATGCCAAGATCAGTGATTTCGGACT
CTCCAAAGCACTGCGTGCTGATGAAAACTACTACAAGGCCCAGACCCATG
GAAAGTGGCCTGTCAAGTGGTACGCTCCGGAATGCATCAACTACTACAAG
TTCTCCAGCAAAAGCGATGTCTGGAGCTTTGGAGTGTTGATGTGGGAAGC
ATTCTCCTATGGGCAGAAGCCATATCGAGGGATGAAAGGAAGTGAAGTCA
CCGCTATGTTAGAGAAAGGAGAGCGGATGGGGTGCCCTGCAGGGTGTCCA
AGAGAGATGTACGATCTCATGAATCTGTGCTGGACATACGATGTGGAAAA
CAGGCCCGGATTCGCAGCAGTGGAACTGCGGCTGCGCAATTACTACTATG
ACGTGGTGAACTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMASSGMADSANHLPFFFGNITR
EEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIERELNG
TYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGPFED
LKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGK
ISREESEQIVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVLHYRIDKDK
TGKLSIPEGKKFDTLWQLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGG
RPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNP
YEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTL
EDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEAN
VMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDKNII
ELVHQVSMGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRAD
ENYYKAQTHGKWPVKWYAPECINYYKFSSKSDVWSFGVLMWEAFSYGQKP
YRGMKGSEVTAMLEKGERMGCPAGCPREMYDLMNLCWTYDVENRPGFAAV
ELRLRNYYYDVVN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8829
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 75435 Daltons