cDNA Clone RSK2

• Synonyms: RPS6KA3, MAPKAP-K1b
• Plasmid: pFB1 6His RSK2 new
• Parent Plasmid: pFastbac1
• Expressed: 6His RSK2 new
• Genbank: NP_004577
• Tag: 6His

Sequence of Insert

CCATGGCGCATCACCATCACCATCACGGATCCCCGCTGGCGCAGCTGGCG
GACCCGTGGCAGAAGATGGCTGTGGAGAGCCCGTCCGACAGCGCTGAGAA
TGGACAGCAAATTATGGATGAACCTATGGGAGAGGAGGAGATTAACCCAC
AAACTGAAGAAGGCAGTATCAAAGAAATTGCAATCACACATCATGTAAAG
GAAGGACATGAAAAGGCAGATCCTTCCCAGTTTGAACTTTTAAAAGTATT
AGGGCAGGGATCATTTGGAAAGGTTTTCTTAGTTAAAAAAATCTCAGGCT
CTGATGCTAGGCAGCTTTATGCCATGAAGGTATTGAAGAAGGCCACACTG
AAAGTTCGAGACCGAGTTCGGACAAAAATGGAACGTGATATCTTGGTAGA
GGTTAATCATCCTTTTATTGTCAAGTTGCATTATGCTTTTCAAACTGAAG
GGAAGTTGTATCTTATTTTGGATTTTCTCAGGGGAGGAGATTTGTTTACA
CGCTTATCCAAAGAGGTGATGTTCACAGAAGAAGATGTCAAATTCTACTT
GGCTGAACTTGCACTTGCTTTAGACCATCTACATAGCCTGGGAATAATTT
ATAGAGACTTAAAACCAGAAAATATACTTCTTGATGAAGAAGGTCACATC
AAGTTAACAGATTTCGGCCTAAGTAAAGAGTCTATTGACCATGAAAAGAA
GGCATATTCTTTTTGTGGAACTGTGGAGTATATGGCTCCAGAAGTAGTTA
ATCGTCGAGGTCATACTCAGAGTGCTGACTGGTGGTCTTTTGGTGTGTTA
ATGTTTGAAATGCTTACTGGTACACTACCTTTCCAAGGAAAAGATCGAAA
AGAAACAATGACTATGATTCTTAAAGCCAAACTTGGAATGCCACAGTTTT
TGAGTCCTGAAGCGCAGAGTCTTTTACGAATGCTTTTCAAGCGAAATCCT
GCAAACAGATTAGGTGCAGGACCAGATGGAGTTGAAGAAATTAAAAGACA
TTCATTTTTCTCAACGATAGACTGGAATAAACTGTATAGAAGAGAAATTC
ATCCGCCATTTAAACCTGCAACGGGCAGGCCTGAAGATACATTCTATTTT
GATCCTGAGTTTACTGCAAAAACTCCCAAAGATTCACCTGGCATTCCACC
TAGTGCTAATGCACATCAGCTTTTTCGGGGGTTTAGTTTTGTTGCTATTA
CCTCAGATGATGAAAGCCAAGCTATGCAGACAGTTGGTGTACATTCAATT
GTTCAGCAGTTACACAGGAACAGTATTCAGTTTACTGATGGATATGAAGT
AAAAGAAGATATTGGAGTTGGCTCCTACTCTGTTTGCAAGAGATGTATAC
ATAAAGCTACAAACATGGAGTTTGCAGTGAAGATTATTGATAAAAGCAAG
AGAGACCCAACAGAAGAAATTGAAATTCTTCTTCGTTATGGACAGCATCC
AAACATTATCACTCTAAAGGATGTATATGATGATGGAAAGTATGTGTATG
TAGTAACAGAACTTATGAAAGGAGGTGAATTGCTGGATAAAATTCTTAGA
CAAAAATTTTTCTCTGAACGAGAGGCCAGTGCTGTCCTGTTCACTATAAC
TAAAACCGTTGAATATCTTCACGCACAAGGGGTGGTTCATAGAGACTTGA
AACCTAGCAACATTCTTTATGTGGATGAATCTGGTAATCCGGAATCTATT
CGAATTTGTGATTTTGGCTTTGCAAAACAGCTGAGAGCGGAAAATGGTCT
TCTCATGACTCCTTGTTACACTGCAAATTTTGTTGCACCAGAGGTTTTAA
AAAGACAAGGCTATGATGCTGCTTGTGATATATGGAGTCTTGGTGTCCTA
CTCTATACAATGCTTACCGGTTACACTCCATTTGCAAATGGTCCTGATGA
TACACCAGAGGAAATATTGGCACGAATAGGTAGCGGAAAATTCTCACTCA
GTGGTGGTTACTGGAATTCTGTTTCAGACACAGCAAAGGACCTGGTGTCA
AAGATGCTTCATGTAGACCCTCATCAGAGACTGACTGCTGCTCTTGTGCT
CAGACATCCTTGGATCGTCCACTGGGACCAACTGCCACAATACCAACTAA
ACAGACAGGATGCACCACATCTAGTAAAGGGTGCCATGGCAGCTACATAT
TCTGCTTTGAACCGTAATCAGTCACCAGTTTTGGAACCAGTAGGCCGCTC
TACTCTTGCTCAGCGGAGAGGTATTAAAAAAATCACCTCAACAGCCCTGT
GAAGTGACCTCAGTGAGATATTTGGATCCATGGTGTAAGCGGCCGC

Amino Acid Sequence

MAHHHHHHGSPLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQ
TEEGSIKEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKISGS
DARQLYAMKVLKKATLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEG
KLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIY
RDLKPENILLDEEGHIKLTDFGLSKESIDHEKKAYSFCGTVEYMAPEVVN
RRGHTQSADWWSFGVLMFEMLTGTLPFQGKDRKETMTMILKAKLGMPQFL
SPEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHSFFSTIDWNKLYRREIH
PPFKPATGRPEDTFYFDPEFTAKTPKDSPGIPPSANAHQLFRGFSFVAIT
SDDESQAMQTVGVHSIVQQLHRNSIQFTDGYEVKEDIGVGSYSVCKRCIH
KATNMEFAVKIIDKSKRDPTEEIEILLRYGQHPNIITLKDVYDDGKYVYV
VTELMKGGELLDKILRQKFFSEREASAVLFTITKTVEYLHAQGVVHRDLK
PSNILYVDESGNPESIRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLK
RQGYDAACDIWSLGVLLYTMLTGYTPFANGPDDTPEEILARIGSGKFSLS
GGYWNSVSDTAKDLVSKMLHVDPHQRLTAALVLRHPWIVHWDQLPQYQLN
RQDAPHLVKGAMAATYSALNRNQSPVLEPVGRSTLAQRRGIKKITSTAL*
SDLSEIFGSMV*

• Vector Type: Baculovirus
• DU Number: DU8708
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression. Differs to genbank at V45G.; ; Estimated MW of expressed protein : 86037 Daltons