cDNA Clone TACE (mouse)

• Plasmid: pHR SIN TACE FLAG
• Parent Plasmid: pHR SIN CSGW
• Expressed: TACE FLAG
• Genbank: BAE23464.1
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GGATCCGCCACCATGAGGCGGCGTCTCCTCATCCTGACCACTTTGGTGCC
TTTCGTCCTGGCACCCCGACCTCCGGAGGAAGCAGGCTCTGGCTCCCATC
CGCGACTTGAGAAGCTTGATTCTTTGCTCTCAGACTACGACATCCTCTCC
TTAGCTAATATTCAGCAGCACTCCATAAGGAAAAGGGATCTACAGTCTGC
GACACACTTAGAAACATTACTAACTTTTTCAGCTTTGAAAAGACATTTTA
AATTATACTTGACATCAAGTACCGAACGCTTTTCACAAAACTTGAGAGTC
GTGGTGGTGGACGGGAAAGAAGAAAGCGAGTACAGCGTGAAGTGGCAGAA
CTTCTTCAGTGGTCACGTGGTTGGTGAGCCTGACTCTAGGGTTCTAGCCC
ACATAGGAGATGATGATGTTACAGTGAGAATCAACACAGATGGGGCAGAA
TATAACGTAGAGCCACTTTGGAGGTTTGTCAATGATACTAAAGATAAACG
AATGCTGGTGTATAAGTCTGAAGATATCAAGGATTTTTCACGTTTGCAGT
CTCCAAAAGTATGTGGTTATTTAAATGCAGATAGTGAAGAGCTGCTTCCA
AAAGGGCTCATAGACAGAGAGCCATCTGAAGAGTTTGTTCGTCGAGTGAA
GAGACGAGCTGAACCTAACCCCTTGAAGAATACTTGTAAATTACTGGTGG
TAGCAGATCATCGATTTTATAAATACATGGGCCGTGGAGAAGAGAGCACC
ACTACAAATTACTTAATAGAGCTAATTGACCGAGTTGATGACATATACCG
GAACACGTCGTGGGATAATGCAGGGTTTAAAGGGTATGGAGTGCAGATAG
AGCAGATTCGAATTCTCAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGA
CACTTCAATATGGCAAAAAGTTTCCCAAACGAAGAGAAGGATGCTTGGGA
TGTGAAGATGCTATTAGAGCAATTTAGCTTTGATATAGCTGAAGAAGCAT
CTAAAGTCTGCCTGGCTCATCTTTTCACGTACCAGGATTTTGATATGGGA
ACTCTTGTATTAGCTTACGTTGGTTCTCCCAGAGCAAACAGTCATGGAGG
GGTTTGTCCAAAAGCTTATTACAACCCAACTGTGAAGAAAAACATCTATT
TAAATAGTGGTCTGACTAGTACTAAAAATTATGGCAAAACTATTCTCACA
AAGGAAGCTGACCTGGTTACAACTCATGAATTGGGACATAATTTTGGAGC
AGAACATGACCCTGATGGGCTAGCAGAATGTGCCCCAAATGAGGACCAAG
GAGGAAAGTATGTCATGTATCCCATAGCTGTGAGCGGTGACCACGAGAAT
AATAAGATGTTTTCAAACTGCAGTAAACAGTCCATCTACAAGACCATAGA
AAGTAAGGCTCAAGAGTGCTTCCAGGAGCGCAGCAACAAGGTGTGTGGCA
ACTCCAGGGTGGATGAAGGAGAGGAGTGTGACCCGGGTATTATGTACCTG
AACAACGACACCTGCTGCAATAGTGACTGCACACTGAAGCCGGGTGTGCA
GTGCAGTGATAGGAACAGTCCTTGCTGTAAAAACTGTCAGTTTGAGACGG
CGCAGAAGAAGTGCCAGGAGGCTATTAATGCTACATGCAAAGGAGTGTCT
TACTGCACAGGGAATAGCAGTGAGTGCCCCCCACCCGGAGATGCTGAAGA
TGACACTGTGTGCTTGGACCTTGGCAAGTGCAAGGCTGGGAAATGCATCC
CTTTCTGCAAGAGGGAGCAGGAGCTGGAGTCCTGCGCATGCGTTGACACT
GACAACTCGTGCAAGGTGTGCTGCAGGAACCTTTCTGGCCCGTGTGTGCC
GTACGTCGATGCAGAGCAAAAGAACTTGTTTTTGAGGAAAGGGAAGCCAT
GTACAGTAGGGTTTTGCGACATGAATGGCAAATGTGAGAAACGAGTACAG
GACGTAATTGAGCGATTTTGGGATTTCATTGACCAGCTGAGCATCAACAC
TTTTGGGAAGTTTCTGGCAGATAACATCGTTGGGTCTGTTCTGGTTTTCT
CCTTGATATTTTGGATTCCTTTCAGCATTCTTGTCCACTGTGTGGATAAG
AAACTGGACAAGCAGTATGAATCCCTGTCTCTGTTTCATCACAGTAACAT
TGAGATGCTGAGCAGCATGGACTCAGCATCTGTTCGCATCATCAAGCCCT
TTCCTGCACCCCAGACTCCAGGTCGTCTGCAGGCCCTGCAGCCAGCTGCC
ATGATGCCGCCAGTACCTGCAGCTCCAAAACTGGACCACCAGAGGATGGA
CACCATCCAGGAAGACCCCAGCACAGACTCACATGCAGATGATGACGGTT
TTGAGAAGGACCCCTTCCCCAACAGCAGCACAGCTGCCAAGTCCTTTGAG
GATCTCACAGACCACCCAGTCACCAGGAGCGAAAAGGCGGCCTCATTCAA
GCTGCAGCGTCAGAGCCGAGTTGACAGCAAAGAGATAGAGTGCGACTACA
AGGACGACGATGACAAGTAAGGATCC

Amino Acid Sequence

MRRRLLILTTLVPFVLAPRPPEEAGSGSHPRLEKLDSLLSDYDILSLANI
QQHSIRKRDLQSATHLETLLTFSALKRHFKLYLTSSTERFSQNLRVVVVD
GKEESEYSVKWQNFFSGHVVGEPDSRVLAHIGDDDVTVRINTDGAEYNVE
PLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLI
DREPSEEFVRRVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNY
LIELIDRVDDIYRNTSWDNAGFKGYGVQIEQIRILKSPQEVKPGERHFNM
AKSFPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLVL
AYVGSPRANSHGGVCPKAYYNPTVKKNIYLNSGLTSTKNYGKTILTKEAD
LVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMF
SNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDT
CCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTG
NSSECPPPGDAEDDTVCLDLGKCKAGKCIPFCKREQELESCACVDTDNSC
KVCCRNLSGPCVPYVDAEQKNLFLRKGKPCTVGFCDMNGKCEKRVQDVIE
RFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDK
QYESLSLFHHSNIEMLSSMDSASVRIIKPFPAPQTPGRLQALQPAAMMPP
VPAAPKLDHQRMDTIQEDPSTDSHADDDGFEKDPFPNSSTAAKSFEDLTD
HPVTRSEKAASFKLQRQSRVDSKEIECDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8672
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning intermediate.