cDNA Clone DDI2

• Plasmid: pCMV-HA-1 DDI2
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-DDI1
• Genbank: NP_115717.3
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCTGCTCACCGTGTACTGTGTGCGGAGGGACCTCTCCGAGGT
GACCTTTTCCCTCCAGGTCGACGCCGACTTCGAGCTGCACAACTTCCGCG
CGCTGTGCGAGCTCGAGTCTGGCATCCCCGCAGCCGAGAGCCAGATCGTC
TATGCGGAAAGACCTCTCACAGACAACCACAGATCATTGGCTTCTTATGG
CTTGAAAGATGGGGACGTTGTGATTTTACGACAGAAGGAGAATGCAGACC
CTCGACCTCCAGTGCAGTTCCCAAACTTACCCCGAATAGATTTCAGTAGT
ATAGCTGTGCCTGGCACATCAAGTCCCCGGCAGCGCCAGCCACCAGGAAC
ACAGCAGTCCCACTCATCTCCTGGAGAAATAACTTCATCTCCTCAGGGCT
TGGACAATCCAGCCTTGCTCCGAGATATGTTGCTGGCCAACCCGCATGAG
CTGTCCTTGCTGAAGGAACGCAATCCACCCCTGGCAGAAGCTCTGCTCAG
TGGAGACCTTGAGAAATTTTCTAGAGTCCTGGTGGAGCAGCAGCAGGACC
GAGCCCGGAGAGAGCAAGAAAGGATTCGTCTGTTTTCTGCTGATCCCTTT
GACCTTGAAGCTCAGGCAAAGATAGAAGAAGATATAAGGCAACAGAACAT
TGAGGAAAACATGACAATAGCTATGGAAGAGGCTCCGGAAAGTTTTGGCC
AAGTAGTGATGCTTTATATTAACTGCAAAGTGAATGGACATCCTGTGAAA
GCCTTTGTTGACTCAGGTGCCCAGATGACTATCATGAGCCAAGCTTGTGC
AGAAAGGTGTAACATAATGAGACTGGTGGACCGTCGGTGGGCAGGGATTG
CCAAAGGAGTGGGCACCCAGAAGATTATTGGAAGGGTACATCTAGCTCAG
GTTCAGATTGAAGGAGATTTTTTGCCATGTTCCTTCTCTATACTTGAGGA
ACAGCCCATGGACATGCTTCTGGGACTGGACATGCTTAAACGGCACCAGT
GTTCCATCGACCTGAAGAAAAATGTACTCGTGATCGGCACCACAGGCTCC
CAGACCACCTTTCTTCCTGAGGGAGAGCTACCAGAGTGTGCCCGGTTGGC
ATATGGGGCTGGAAGAGAGGATGTACGGCCAGAGGAGATTGCAGACCAAG
AATTAGCAGAAGCCCTTCAAAAATCAGCAGAGGATGCAGAGCGTCAGAAG
CCATGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMLLTVYCVRRDLSEVTFSLQVDADFELHNFRALCELES
GIPAAESQIVYAERPLTDNHRSLASYGLKDGDVVILRQKENADPRPPVQF
PNLPRIDFSSIAVPGTSSPRQRQPPGTQQSHSSPGEITSSPQGLDNPALL
RDMLLANPHELSLLKERNPPLAEALLSGDLEKFSRVLVEQQQDRARREQE
RIRLFSADPFDLEAQAKIEEDIRQQNIEENMTIAMEEAPESFGQVVMLYI
NCKVNGHPVKAFVDSGAQMTIMSQACAERCNIMRLVDRRWAGIAKGVGTQ
KIIGRVHLAQVQIEGDFLPCSFSILEEQPMDMLLGLDMLKRHQCSIDLKK
NVLVIGTTGSQTTFLPEGELPECARLAYGAGREDVRPEEIADQELAEALQ
KSAEDAERQKP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8661
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 45880 Daltons