cDNA Clone DDI2

• Plasmid: pEBG6P-DDI2
• Parent Plasmid: pEBG6P
• Expressed: GST-DDI1
• Genbank: NP_115717.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCTGCTCACCGTGTACTGTGTGCGGAGGGACCTCTCCGAGGT
GACCTTTTCCCTCCAGGTCGACGCCGACTTCGAGCTGCACAACTTCCGCG
CGCTGTGCGAGCTCGAGTCTGGCATCCCCGCAGCCGAGAGCCAGATCGTC
TATGCGGAAAGACCTCTCACAGACAACCACAGATCATTGGCTTCTTATGG
CTTGAAAGATGGGGACGTTGTGATTTTACGACAGAAGGAGAATGCAGACC
CTCGACCTCCAGTGCAGTTCCCAAACTTACCCCGAATAGATTTCAGTAGT
ATAGCTGTGCCTGGCACATCAAGTCCCCGGCAGCGCCAGCCACCAGGAAC
ACAGCAGTCCCACTCATCTCCTGGAGAAATAACTTCATCTCCTCAGGGCT
TGGACAATCCAGCCTTGCTCCGAGATATGTTGCTGGCCAACCCGCATGAG
CTGTCCTTGCTGAAGGAACGCAATCCACCCCTGGCAGAAGCTCTGCTCAG
TGGAGACCTTGAGAAATTTTCTAGAGTCCTGGTGGAGCAGCAGCAGGACC
GAGCCCGGAGAGAGCAAGAAAGGATTCGTCTGTTTTCTGCTGATCCCTTT
GACCTTGAAGCTCAGGCAAAGATAGAAGAAGATATAAGGCAACAGAACAT
TGAGGAAAACATGACAATAGCTATGGAAGAGGCTCCGGAAAGTTTTGGCC
AAGTAGTGATGCTTTATATTAACTGCAAAGTGAATGGACATCCTGTGAAA
GCCTTTGTTGACTCAGGTGCCCAGATGACTATCATGAGCCAAGCTTGTGC
AGAAAGGTGTAACATAATGAGACTGGTGGACCGTCGGTGGGCAGGGATTG
CCAAAGGAGTGGGCACCCAGAAGATTATTGGAAGGGTACATCTAGCTCAG
GTTCAGATTGAAGGAGATTTTTTGCCATGTTCCTTCTCTATACTTGAGGA
ACAGCCCATGGACATGCTTCTGGGACTGGACATGCTTAAACGGCACCAGT
GTTCCATCGACCTGAAGAAAAATGTACTCGTGATCGGCACCACAGGCTCC
CAGACCACCTTTCTTCCTGAGGGAGAGCTACCAGAGTGTGCCCGGTTGGC
ATATGGGGCTGGAAGAGAGGATGTACGGCCAGAGGAGATTGCAGACCAAG
AATTAGCAGAAGCCCTTCAAAAATCAGCAGAGGATGCAGAGCGTCAGAAG
CCATGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMLLTVYCVRRDLS
EVTFSLQVDADFELHNFRALCELESGIPAAESQIVYAERPLTDNHRSLAS
YGLKDGDVVILRQKENADPRPPVQFPNLPRIDFSSIAVPGTSSPRQRQPP
GTQQSHSSPGEITSSPQGLDNPALLRDMLLANPHELSLLKERNPPLAEAL
LSGDLEKFSRVLVEQQQDRARREQERIRLFSADPFDLEAQAKIEEDIRQQ
NIEENMTIAMEEAPESFGQVVMLYINCKVNGHPVKAFVDSGAQMTIMSQA
CAERCNIMRLVDRRWAGIAKGVGTQKIIGRVHLAQVQIEGDFLPCSFSIL
EEQPMDMLLGLDMLKRHQCSIDLKKNVLVIGTTGSQTTFLPEGELPECAR
LAYGAGREDVRPEEIADQELAEALQKSAEDAERQKP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8659
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression.; ; Estimated MW of expressed protein : 71938 Daltons