cDNA Clone MAPK12

• Synonyms: ERK3; ERK6; ERK-6; SAPK3; PRKM12; SAPK-3; MAPK 12; P38GAMMA
• Plasmid: pET28b MAPK12
• Parent Plasmid: pET28b original
• Expressed: 6His MAPK12
• Genbank: NM_002969
• Tag: 6His
• Tag: T7
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGCTCTCCGCCGCCCGCCCGCAGTGGCTTTTACCGCCA
GGAGGTAACCAAGACGGCCTGGGAGGTGCGCGCCGTATACCGGGACCTGC
AGCCCGTGGGCTCGGGCGCCTACGGCGTGGTGTGCTCGGCCGTGGACGGC
CGCACCGGCGCTAAGGTGGCCATCAAGAAGCTGTATCGGCCCTTCCAGTC
CGAGCTGTTCGCCAAGCGCGCCTACCGCGAGCTGCGCCTGCTCAAGCACA
TGCGCCACGAGAACGTGATCGGGCTGCTGGACGTATTCACTCCTGATGAG
ACCCTGGATGACTTCACGGACTTTTACCTGGTGATGCCGTTCATGGGCAC
CGACCTGGGCAAGCTCATGAAACATGAGAAGCTAGGCGAGGACCGGATCC
AGTTCCTCGTGTACCAGATGCTGAAGGGGCTGAGGTATATCCACGCTGCC
GGCATCATCCACAGAGACCTGAAGCCCGGCAACCTGGCTGTGAACGAAGA
CTGTGAGCTGAAGATCCTGGACTTCGGCCTGGCCAGGCAGGCAGACAGTG
AGATGACTGGGTACGTGGTGACCCGGTGGTACCGGGCTCCCGAGGTCATC
TTGAATTGGATGCGCTACACGCAGACGGTGGACATCTGGTCTGTGGGCTG
CATCATGGCGGAGATGATCACAGGCAAGACGCTGTTCAAGGGCAGCGACC
ACCTGGACCAGCTGAAGGAGATCATGAAGGTGACGGGGACGCCTCCGGCT
GAGTTTGTGCAGCGGCTGCAGAGCGATGAGGCCAAGAACTACATGAAGGG
CCTCCCCGAATTGGAGAAGAAGGATTTTGCCTCTATCCTGACCAATGCAA
GCCCTCTGGCTGTGAACCTCCTGGAGAAGATGCTGGTGCTGGACGCGGAG
CAGCGGGTGACGGCAGGCGAGGCGCTGGCCCATCCCTACTTCGAGTCCCT
GCACGACACGGAAGATGAGCCCCAGGTCCAGAAGTATGATGACTCCTTTG
ACGACGTTGACCGCACACTGGATGAATGGAAGCGTGTTACTTACAAAGAG
GTGCTCAGCTTCAAGCCTCCCCGGCAGCTGGGGGCCAGGGTCTCCAAGGA
GACGCCTCTGTGAGCGGCCGCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRDPNSSSVDKLAAAMSSPP
PARSGFYRQEVTKTAWEVRAVYRDLQPVGSGAYGVVCSAVDGRTGAKVAI
KKLYRPFQSELFAKRAYRELRLLKHMRHENVIGLLDVFTPDETLDDFTDF
YLVMPFMGTDLGKLMKHEKLGEDRIQFLVYQMLKGLRYIHAAGIIHRDLK
PGNLAVNEDCELKILDFGLARQADSEMTGYVVTRWYRAPEVILNWMRYTQ
TVDIWSVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQS
DEAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEA
LAHPYFESLHDTEDEPQVQKYDDSFDDVDRTLDEWKRVTYKEVLSFKPPR
QLGARVSKETPL*

• Vector Type: Bacterial
• Antibiotic: Kan
• Full Snapgene Map: pET28b MAPK1262.12 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU8642
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for bacterial expression.; ; Estimated MW of expressed protein : 46622 Daltons
• Low Temp: No