cDNA Clone MEKK3

• Synonyms: MAP3K3
• Plasmid: pGFP TAPP MEKK3
• Parent Plasmid: pEGFP TAP
• Expressed: GFP-TAP-MEKK3
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GCGGCCGCGGACGAACAGGAGGCATTGAACTCAATCATGAACGATCTGGT
GGCCCTCCGGATGAACCGACGTCACCGGATGCCTGGATATGAGACCATGA
AGAACAAAGACACAGGTCACTCAAATAGGCAGAGTGACGTCAGAATCAAG
TTCGAGCACAACGGGGAGAGGCGAATTATAGCGTTCAGCCGGCCTGTGAA
ATATGAAGATGTGGAGCACAAGGTGACAACAGTATTTGGACAACCTCTTG
ATCTACATTACATGAACAATGAGCTCTCCATCCTGCTGAAAAACCAAGAT
GATCTTGATAAAGCAATTGACATTTTAGATAGAAGCTCAAGCATGAAAAG
CCTTAGGATATTGCTGTTGTCCCAGGACAGAAACCATAACAGTTCCTCTC
CCCACTCTGGGGTGTCCAGACAGGTGCGGATCAAGGCTTCCCAGTCCGCA
GGGGATATAAATACTATCTACCAGCCCCCCGAGCCCAGAAGCAGGCACCT
CTCTGTCAGCTCCCAGAACCCTGGCCGAAGCTCACCTCCCCCTGGCTATG
TTCCTGAGCGGCAGCAGCACATTGCCCGGCAGGGGTCCTACACCAGCATC
AACAGTGAGGGGGAGTTCATCCCAGAGACCAGCGAGCAGTGCATGCTGGA
TCCCCTGAGCAGTGCAGAAAATTCCTTGTCTGGAAGCTGCCAATCCTTGG
ACAGCCCATCCTTCCGGAAATCACGAATGTCCCGTGCCCAGAGCTTCCCT
GACAACAGACAGGAATACTCAGATCGGGAAACTCAGCTTTATGACAAAGG
GGTCAAAGGTGGAACCTACCCCCGGCGCTACCACGTGTCTGTGCACCACA
AGGACTACAGTGATGGCAGAAGAACATTTCCCCGAATACGGCGTCATCAA
GGCAACTTGTTCACCCTGGTGCCCTCCAGCCGCTCCCTGAGCACAAATGG
CGAGAACATGGGCCTGGCTGTGCAATACCTGGACCCCCGTGGGCGCCTGC
GGAGTGCGGACAGCGAGAATGCCCTCTCTGTGCAGGAGAGGAATGTGCCA
ACCAAGTCTCCCAGTGCCCCCATCAACTGGCGCCGGGGAAAGCTCCTGGG
CCAGGGTGCCTTCGGCAGGGTCTATTTGTGCTATGACGTGGACACGGGAC
GTGAACTTGCTTCCAAGCAGGTCCAATTTGATCCAGACAGTCCTGAGACA
AGCAAGGAGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAACTT
GCAGCATGAGCGCATCGTGCAGTACTATGGCTGTCTGCGGGACCGCGCTG
AGAAGACCCTGACCATCTTCATGGAGTACATGCCAGGGGGCTCGGTGAAA
GACCAGTTGAAGGCTTACGGTGCTCTGACAGAGAGCGTGACCCGAAAGTA
CACGCGGCAGATCCTGGAGGGCATGTCCTACCTGCACAGCAACATGATTG
TTCACCGGGACATTAAGGGAGCCAACATCCTCCGAGACTCTGCTGGGAAT
GTAAAGCTGGGGGACTTTGGGGCCAGCAAACGCCTGCAGACGATCTGTAT
GTCGGGGACGGGCATGCGCTCCGTCACTGGCACACCCTACTGGATGAGCC
CTGAGGTGATCAGCGGCGAGGGCTATGGAAGGAAAGCAGACGTGTGGAGC
CTGGGCTGCACTGTGGTGGAGATGCTGACAGAGAAACCACCGTGGGCAGA
GTATGAAGCTATGGCCGCCATCTTCAAGATTGCCACCCAGCCCACCAATC
CTCAGCTGCCCTCCCACATCTCTGAACATGGCCGGGACTTCCTGAGGCGC
ATTTTTGTGGAGGCTCGCCAGAGACCTTCAGCTGAGGAGCTGCTCACACA
CCACTTTGCACAGCTCATGTACTGAGAATTCGCAAGGGCGAATTCGCGGC
CGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSRSG
TAAADEQEALNSIMNDLVALRMNRRHRMPGYETMKNKDTGHSNRQSDVRI
KFEHNGERRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQ
DDLDKAIDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQS
AGDINTIYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTS
INSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDSPSFRKSRMSRAQSF
PDNRQEYSDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRH
QGNLFTLVPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENALSVQERNV
PTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPE
TSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSV
KDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAG
NVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVW
SLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLR
RIFVEARQRPSAEELLTHHFAQLMY*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8629
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GFP TAPP for mammalian expression.; ; Estimated MW of expressed protein : 98699 Daltons