cDNA Clone PIAS3

• Plasmid: CFP-C1 PIAS3
• Parent Plasmid: pECFPC1
• Expressed: CFP-PIAS3
• Genbank: AAH51252
• Tag: CFP
• Species: Mouse

Sequence of Insert

GAATTCGCCCTTGCGCGGCCGCGATGGCGGAGCTGGGCGAGTTAAAGCAC
ATGGTGATGAGTTTCCGAGTGTCTGAGCTCCAGGTGCTCCTCGGCTTCGC
TGGCAGGAACAAGAGTGGGCGGAAACACGAGCTGCTGGCCAAGGCCCTGC
ACCTCCTCAAGTCTAGCTGCGCCCCCAGCGTCCAGATGAAGATCAAAGAA
CTTTATCGCAGGCGCTTTCCCCGGAAGACCCTGGGGCCTTCTGATCTCTC
CTTGCTTTCTTTGCCCCCTGGCACCTCTCCTGTAGGCTCCCCCGGCCCCC
TTGCTCCCATCCCTCCCACCCTTCTGACCCCTGGCACCTTGCTAGGCCCT
AAGCGTGAGGTGGACATGCATCCTCCTCTGCCGCAGCCTGTGCACCCCGA
TGTCACCATGAAGCCACTGCCCTTCTATGAAGTCTATGGGGAGCTCATCC
GACCCACCACCCTTGCGTCCACCTCCAGCCAGAGGTTCGAGGAAGCCCAC
TTCACCTTCGCGCTCACTCCCCAGCAGCTGCAGCAGATTCTCACGTCCAG
GGAGGTTCTGCCAGGAGCCAAGTGTGATTACACCATACAAGTGCAGCTCA
GATTCTGTCTCTGTGAGACCAGCTGCCCTCAGGAGGACTATTTCCCCCCT
AACCTCTTTGTTAAGGTTAATGGGAAACTCTGCCCCCTGCCGGGTTACCT
CCCTCCAACCAAGAATGGAGCTGAGCCCAAGAGGCCCAGCCGTCCGATCA
ACATCACACCCTTGGCTCGACTCTCAGCCACTGTCCCCAACACCATCGTA
GTTAATTGGTCATCTGAGTTTGGACGGAATTACTCCTTGTCCGTGTACCT
GGTGAGGCAATTGACTGCAGGGACCCTTCTACAAAAACTCAGAGCCAAGG
GGATCCGGAATCCAGACCATTCCCGGGCACTGATCAAGGAGAAACTGACT
GCTGACCCCGACAGTGAAGTGGCTACTACAAGTCTCCGGGTGTCACTCAT
GTGCCCGCTAGGGAAGATGCGCCTGACTGTCCCGTGTCGTGCCCTCACCT
GTGCCCATCTGCAGAGTTTCGATGCTGCCCTTTATCTACAGATGAATGAG
AAGAAGCCGACATGGACGTGTCCTGTGTGTGACAAGAAGGCTCCCTATGA
GTCGCTGATTATTGATGGTTTATTCATGGAAATTCTTAATTCCTGTTCGG
ATTGTGATGAGATCCAGTTCATGGAAGATGGATCCTGGTGTCCGATGAAA
CCCAAGAAGGAGGCATCAGAGGTTTGCCCCCCGCCAGGGTATGGGCTGGA
TGGTCTCCAGTACAGCGCAGTCCAGGAGGGAATTCAGCCAGAGAGTAAGA
AGAGGGTCGAAGTCATTGACTTGACCATCGAAAGCTCATCAGATGAGGAG
GATTTGCCCCCCACCAAGAAGCACTGCCCTGTCACCTCAGCGGCCATTCC
AGCCCTTCCTGGAAGCAAAGGAGCCCTGACCTCTGGTCACCAGCCATCCT
CGGTGCTGCGGAGCCCTGCAATGGGCACACTGGGCAGTGACTTCCTGTCT
AGTCTCCCGCTACATGAGTACCCACCTGCCTTCCCACTGGGGGCTGACAT
CCAAGGTTTAGATTTATTTTCTTTCCTTCAGACTGAGAGTCAGCACTACG
GCCCTTCAGTTATCACTTCGCTAGATGAACAGGACACCCTTGGCCACTTC
TTCCAGTACCGGGGAACCCCTTCCCACTTCCTGGGCCCACTGGCCCCCAC
ACTGGGGAGCTCTCACCGCAGCTCCACTCCAGCGCCCCCTCCTGGTCGTG
TCAGCAGCATTGTGGCTCCTGGGAGCTCCTTGAGGGAAGGGCATGGAGGA
CCCCTGCCTTCAGGTCCCTCTTTGACTGGCTGTCGGTCAGACGTCATTTC
CTTGGACTGAGCGGCCGCGAATTC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAAASV
MDICRIRPCAAAMAELGELKHMVMSFRVSELQVLLGFAGRNKSGRKHELL
AKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVG
SPGPLAPIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVY
GELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTI
QVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRP
SRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQK
LRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPC
RALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEIL
NSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSAVQEGIQ
PESKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSG
HQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTE
SQHYGPSVITSLDEQDTLGHFFQYRGTPSHFLGPLAPTLGSSHRSSTPAP
PPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8589
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal CFP for mammalian expression.; ; Estimated MW of expressed protein : 97887 Daltons