cDNA Clone PIAS1

• Plasmid: CFP-C1 PIAS1
• Parent Plasmid: pECFPC1
• Expressed: CFP-PIAS1
• Genbank: BAC35902
• Tag: CFP
• Species: Mouse

Sequence of Insert

AGATCCATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAG
AGTTTCTGAACTCCAAGTACTGTTGGGCTACGCTGGGAGGAACAAGCACG
GACGCAAACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGCTGGC
TGTAGTCCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTT
CCCTCAGAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATT
CAAGTCCTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACT
TATGATGGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGG
ACCCAAACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAG
TCCACCCGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGAT
GAACTGATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCG
GGAAACCTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCA
GCAGCTCCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTC
CAATTAAGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTT
CCCACCCAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAG
GTTACCTTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTAGCCGA
CCAATTAATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATAC
CATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAG
TATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGA
GCAAAGGGAATAAGGAATCCGGATCATTCTAGAGCTTTAATTAAAGAGAA
GTTAACTGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTT
CGCTGCTGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCA
CTTACCTGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAAT
GAATGAGAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCC
CATATGAACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTAC
TGCACAGACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCC
AATGAGGTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAG
TTGATGGTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAAC
CAGTCCTCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGA
CAGCTCGTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTC
CTTCCCTGTCTCCTACGTCACCACTAAGTAATAAAGGCATTTTAAGTCTT
CCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTGTAGA
TACAAGCTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCC
ACATGACGCCTATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTC
TTATCAGGAGACAATCAGCATTACAACACCTCCCTGCTAGCCGCAGCTGC
AGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCC
CGTATACCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGC
ACATCTCTGCCAGCCACCAACGGAAGCAGTAGCGGCAGCAACAGCAGCCT
TGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCCAGCA
GGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATC
TCATTGGACTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMADSAE
LKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMK
IKELYRRRFPQKIMTPADLSIPNVHSSPMPPTLSPSTIPQLTYDGHPASS
PLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSL
ASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSE
TSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLV
RLSTTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPD
HSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQC
FDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQ
FKEDGSWAPMRSKKEVQEVTASYNGVDGCLSSTLEHQVASHNQSSNKNKK
VEVIDLTIDSSSDEEEEEPPAKRTCPSLSPTSPLSNKGILSLPHQASPVS
RTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHY
NTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPATNG
SSSGSNSSLVSSNSLRESHGHGVASRSSADTASIFGIIPDIISLD*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8588
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N Ter CFP for mammalian expression.; ; Estimated MW of expressed protein : 99003 Daltons