cDNA Clone Ubiquitin

• Plasmid: pGEX6P-1-Ubiquitin K29wt K33wt
• Parent Plasmid: pGEX6P1
• Expressed: GST-Ubiquitin K29wt K33wt
• Genbank: P62988
• Tag: GST

Sequence of Insert

GGATCTCAAATCCCCCTGAGGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAGGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAGACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAGAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGTGGGTGATAACTCGACTCGAGCG
GCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSQIPLRTLTGRTITLEVEPS
DTIENVRAKIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLV
LRLRGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8582
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST/FLAG for bacterial expression