cDNA Clone AMPK

• Plasmid: pGEX-AMPK isoform 2
• Parent Plasmid: pGEX6P1
• Expressed: GST-AMPK isoform 2
• Genbank: NP_006242.5
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCGAGAAGCAGAAGCACGACGGGCGGGTGAAGATCGGCCACTACAT
CCTGGGGGACACGCTGGGCGTCGGCACCTTCGGGAAAGTGAAGGTGGGCA
AGCACGAGTTGACTGGACATAAAGTTGCTGTGAAGATACTCAACCGGCAG
AAGATTCGAAGCCTGGACGTGGTCGGGAAAATCCGCAGAGAGATCCAGAA
CCTGAAGCTTTTCAGGCACCCTCATATAATCAAACTGTACCAGGTCATCA
GTACACCGTCTGATATTTTCATGGTCATGGAATATGTCTCAGGAGGAGAG
CTATTTGATTATATCTGTAAAAATGGAAGGTTGGACGAAAAGGAGAGTCG
ACGTCTGTTCCAGCAGATCCTTTCTGGTGTGGACTATTGTCACAGGCATA
TGGTGGTCCACAGAGATTTGAAACCTGAAAACGTCCTGCTTGATGCACAC
ATGAATGCAAAGATAGCCGACTTCGGTCTTTCAAACATGATGTCAGATGG
TGAATTTTTAAGAACGAGCTGTGGCTCGCCCAATTATGCTGCACCAGAAG
TAATTTCAGGAAGATTGTACGCAGGCCCTGAAGTAGACATCTGGAGCAGC
GGGGTCATTCTCTATGCTTTGCTGTGTGGAACTCTCCCTTTTGATGATGA
CCACGTGCCAACTCTTTTTAAGAAGATATGTGACGGGATATTTTATACCC
CTCAGTATTTGAATCCCTCTGTAATAAGCCTTTTGAAGCATATGCTGCAG
GTAGATCCTATGAAGAGGGCCACAATAAAAGATATCAGGGAACATGAATG
GTTTAAGCAGGACCTTCCAAAATATCTCTTTCCTGAAGACCCGTCTTATA
GTTCAACCATGATTGATGATGAAGCCTTAAAAGAAGTGTGTGAGAAGTTC
GAGTGCTCAGAGGAGGAGGTCCTCCACCATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFEKQKHDGRVKIGHYIL
GDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNL
KLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRR
LFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGE
FLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDH
VPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWF
KQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8567
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression. Has an extra HH at C terminus.; ; Estimated MW of expressed protein : 62486 Daltons