cDNA Clone TBK1

• Plasmid: pFBHTb TBK1 S172D
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-TBK1 S172D
• Genbank: NP_037386.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTT
AGGCCAAGGAGCTACTGCAAATGTCTTTCGTGGAAGACATAAGAAAACTG
GTGATTTATTTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCGTCCA
GTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAAAAACTCAATCACAA
AAATATTGTCAAATTATTTGCTATTGAAGAGGAGACAACAACAAGACATA
AAGTACTTATTATGGAATTTTGTCCATGTGGGAGTTTATACACTGTTTTA
GAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGT
TTTGCGAGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAG
TGCACCGTGATATCAAGCCAGGAAATATCATGCGTGTTATAGGGGAAGAT
GGACAGTCTGTGTACAAACTCACAGATTTTGGTGCAGCTAGAGAATTAGA
AGATGATGAGCAGTTTGTTGATCTGTATGGCACAGAAGAATATTTGCACC
CTGATATGTATGAGAGAGCAGTGCTAAGAAAAGATCATCAGAAGAAATAT
GGAGCAACAGTTGATCTTTGGAGCATTGGGGTAACATTTTACCATGCAGC
TACTGGATCACTGCCATTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAG
AAGTGATGTATAAAATAATTACAGGAAAGCCTTCTGGTGCAATATCTGGA
GTACAGAAAGCAGAAAATGGACCAATTGACTGGAGTGGAGACATGCCTGT
TTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTTCTTG
CAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTT
TTTGCAGAAACTAGTGATATACTTCACCGAATGGTAATTCATGTTTTTTC
GCTACAACAAATGACAGCTCATAAGATTTATATACATAGCTATAATACTG
CTACTATATTTCATGAACTGGTATATAAACAAACCAAAATTATTTCTTCA
AATCAAGAACTTATCTACGAAGGGCGACGCTTAGTCTTAGAACCTGGAAG
GCTGGCACAACATTTCCCTAAAACTACTGAGGAAAACCCTATATTTGTAG
TAAGCCGGGAACCTCTGAATACCATAGGATTAATATATGAAAAAATTTCC
CTCCCTAAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGC
TAAGGCAATAACAGGGGTTGTGTGTTATGCCTGCAGAATTGCCAGTACCT
TACTGCTTTATCAGGAATTAATGCGAAAGGGGATACGATGGCTGATTGAA
TTAATTAAAGATGATTACAATGAAACTGTTCACAAAAAGACAGAAGTTGT
GATCACATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAAGTAT
ATGAAAAGTTGATGAAGATCAACCTGGAAGCGGCAGAGTTAGGTGAAATT
TCAGACATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAACAAT
AGAAACCAGTCTTCAGGATATCGACAGCAGATTATCTCCAGGTGGATCAC
TGGCAGACGCATGGGCACATCAAGAAGGCACTCATCCGAAAGACAGAAAT
GTAGAAAAACTACAAGTCCTGTTAAATTGCATGACAGAGATTTACTATCA
GTTCAAAAAAGACAAAGCAGAACGTAGATTAGCTTATAATGAAGAACAAA
TCCACAAATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATG
ACGCACTTTACAGATGAATGTGTTAAAAAGTATGAGGCATTTTTGAATAA
GTCAGAAGAATGGATAAGAAAGATGCTTCATCTTAGGAAACAGTTATTAT
CGCTGACTAATCAGTGTTTTGATATTGAAGAAGAAGTATCAAAATATCAA
GAATATACTAATGAGTTACAAGAAACTCTGCCTCAGAAAATGTTTACAGC
TTCCAGTGGAATCAAACATACCATGACCCCAATTTATCCAAGTTCTAACA
CATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAA
GGGGTGGTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGG
CTCTTTAACCATGGATGGTGGCCTTCGCAACGTTGACTGTCTTTAGGCGG
CCGC

Amino Acid Sequence

MYYHHHHHHDYDIPMTENLYFQGAMGSMQSTSNHLWLLSDILGQGATANV
FRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAI
EEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGM
NHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVDL
YGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRP
FEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGL
QVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHK
IYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKT
TEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVC
YACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIR
NIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDID
SRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAER
RLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKM
LHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTM
TPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMDGGL
RNVDCL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8559
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6HIS for baculovirus expression.; ; Estimated MW of expressed protein : 86982 Daltons