cDNA Clone IKK epsilon

• Plasmid: pCMV-HA IKK epsilon
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-IKK epsilon
• Genbank: NP_054721
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCT
GGGGCAGGGGGCCACTGCCAGTGTGTACAAGGCCCGCAACAAGAAATCCG
GAGAGCTGGTTGCTGTGAAGGTCTTCAACACTACCAGCTACCTGCGGCCC
CGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGGAAGCTGAACCACCA
GAACATCGTCAAGCTCTTTGCGGTGGAGGAGACGGGCGGAAGCCGGCAGA
AGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTG
GAGAGCCCTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGT
GCTGCGCTGTGTGGTGGCCGGCATGAACCACCTGCGGGAGAACGGCATTG
TGCATCGCGACATCAAGCCGGGGAACATCATGCGCCTCGTAGGGGAGGAG
GGGCAGAGCATCTACAAGCTGACAGACTTCGGCGCTGCCCGGGAGCTGGA
TGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTGCATC
CCGACATGTATGAGCGGGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTC
GGGGTGACTGTGGATCTCTGGAGCATTGGAGTGACCTTGTACCATGCAGC
CACTGGCAGCCTGCCCTTCATCCCCTTTGGTGGGCCACGGCGGAACAAGG
AGATCATGTACCGGATCACCACGGAGAAGCCGGCTGGGGCCATTGCAGGT
GCCCAGAGGCGGGAGAACGGGCCCCTGGAGTGGAGCTACACCCTCCCCAT
CACCTGCCAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATCCTGG
CCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTC
TTTGCGGAGACCAGTGACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTC
CCTGTCCCAGGCAGTCCTGCACCACATCTATATCCATGCCCACAACACGA
TAGCCATTTTCCAGGAGGCCGTGCACAAGCAGACCAGTGTGGCCCCCCGA
CACCAGGAGTACCTCTTTGAGGGTCACCTCTGTGTCCTCGAGCCCAGCGT
CTCAGCACAGCACATCGCCCACACGACGGCAAGCAGCCCCCTGACCCTCT
TCAGCACAGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCTGGAC
GTCCCCAAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGC
CAAGGGCGTGTTGGGCGCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCC
TGCTGGATGGGCAGGAGCTAATGTTTCGGGGGCTGCACTGGGTCATGGAG
GTGCTCCAGGCCACATGCAGACGGACTCTGGAAGTGGCAAGGACATCCCT
CCTCTACCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTGGCTG
GAACGCCTGAGATCCAGGAACTGAAGGCGGCTGCAGAACTGAGGTCCAGG
CTGCGGACTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCACGGA
GACCCAGGAGAGCCTGAGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGG
ATCAGGTACATGAGGACAGAAGCATCCAGCAGATTCAGTGCTGTTTGGAC
AAGATGAACTTCATCTACAAACAGTTCAAGAAGTCTAGGATGAGGCCAGG
GCTTGGCTACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAATTTCA
GTCATTTAGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAG
AAGTATCAAGCGTCCTTAGTCACACACGGCAAGAGGATGAGGGTGGTGCA
CGAGACCAGGAACCACCTGCGCCTGGTTGGCTGTTCTGTGGCTGCCTGTA
ACACAGAAGCCCAGGGGGTCCAGGAGAGTCTCAGCAAGCTCCTGGAAGAG
CTATCTCACCAGCTCCTTCAGGACCGAGCAAAGGGGGCTCAGGCCTCGCC
GCCTCCCATAGCTCCTTACCCCAGCCCTACACGAAAGGACCTGCTTCTCC
ACATGCAAGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTG
GACAACAACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACCTCCTGA
TGTCTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVK
VFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEY
CSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKP
GNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERA
VLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRIT
TEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVE
QAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEA
VHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPK
GLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQEL
MFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQE
LKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDR
SIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRL
LQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGV
QESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCE
GMKLLASDLLDNNRIIERLNRVPAPPDV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8531
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 81818 Daltons