cDNA Clone XPO5

• Plasmid: pCMV-HA-2 XPO5
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-XPO5
• Genbank: AAH62635
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGATGGATCAAGTAAACGCGCTGTGCGAGCAGCTGGT
GAAAGCGGTGACGGTCATGATGGACCCCAACTCCACCCAGCGCTACCGGC
TGGAAGCCCTCAAGTTTTGTGAGGAGTTTAAAGAAAAGTGTCCTATCTGT
GTCCCCTGTGGCTTGAGGTTGGCTGAGAAAACACAAGTTGCCATCGTCAG
ACATTTTGGCCTTCAGATCCTGGAACACGTTGTCAAGTTTCGGTGGAACG
GCATGTCTCGATTGGAGAAGGTGTATCTGAAGAACAGTGTCATGGAGCTG
ATTGCAAATGGAACATTGAACATTTTGGAAGAGGAGAACCATATTAAAGA
TGCTCTGTCTCGAATTGTAGTGGAAATGATCAAGCGAGAGTGGCCACAGC
ATTGGCCTGACATGCTAATAGAATTGGACACTCTTTCCAAACAAGGGGAA
ACACAGACAGAATTGGTGATGTTTATCCTTTTGCGACTGGCAGAGGATGT
AGTGACTTTTCAGACACTTCCCCCTCAAAGAAGAAGGGACATCCAGCAAA
CATTAACCCAGAACATGGAAAGGATCTTCAGTTTTCTGCTTAACACACTT
CAAGAAAATGTAAACAAGTATCAGCAAGTGAAGACAGATACTTCTCAGGA
GTCAAAGGCGCAAGCAAACTGTCGAGTAGGAGTTGCAGCACTGAATACTC
TAGCAGGCTATATTGACTGGGTGTCTATGAGTCACATCACTGCTGAAAAC
TGTAAACTCCTGGAGATACTGTGTTTGCTGTTGAATGAACAGGAACTTCA
GTTGGGAGCCGCTGAGTGTCTTCTCATTGCAGTCAGCAGAAAAGGCAAGT
TGGAAGACCGGAAGCCCTTGATGGTCTTATTTGGAGATGTTGCCATGCAT
TATATACTCTCCGCCGCACAGACTGCTGATGGAGGAGGTTTGGTAGAAAA
ACACTACGTCTTTCTGAAGAGGCTCTGTCAGGTGTTGTGTGCGCTGGGCA
ATCAGCTGTGTGCATTGCTGGGTGCAGATTCTGATGTAGAAACACCATCA
AACTTTGGAAAATACCTGGAATCTTTTCTTGCTTTCACAACCCATCCAAG
TCAGTTTCTACGCTCTTCAACTCAGATGACTTGGGGAGCCCTCTTCAGGC
ATGAAATCCTGTCCCGTGATCCTTTGCTATTAGCAATAATACCAAAATAT
CTTCGTGCTTCCATGACTAACTTGGTCAAGATGGGCTTTCCTTCTAAAAC
AGACAGCCCTAGCTGTGAATATTCTCGGTTTGATTTTGATAGCGATGAGG
ACTTCAATGCTTTCTTCAACTCCTCCCGAGCACAACAGGGAGAGGTGATG
AGGTTGGCATGTCGTTTGGATCCCAAAACTAGCTTCCAGATGGCTGGGGA
GTGGCTAAAGTATCAACTATCAACTTTTCTTGATGCTGGTTCTGTGAATT
CTTGTTCTGCAGTTGGAACTGGAGAAGGAAGCCTCTGTTCCGTCTTCTCA
CCTTCATTCGTGCAGTGGGAAGCCATGACTCTTTTTTTGGAAAGTGTTAT
CACCCAGATGTTTCGAACACTAAATAGAGAAGAAATTCCTGTTAATGATG
GAATAGAGCTATTGCAGATGGTTCTGAACTTTGATACCAAGGATCCCCTC
ATCCTGTCCTGCGTCCTTACTAATGTCTCTGCACTCTTTCCATTTGTCAC
CTACAGACCAGAGTTCCTGCCCCAGGTCTTCTCTAAGCTATTTTCATCTG
TCACTTTTGAAACTGTTGAAGAAAGTAAGGCCCCCAGAACCCGGGCAGTG
AGGAATGTGAGGAGGCATGCTTGTTCCTCCATCATCAAGATGTGTCGTGA
CTACCCCCAGCTTGTGCTGCCCAATTTTGACATGCTTTATAACCATGTGA
AGCAACTCCTCTCCAATGAGCTACTCCTGACACAAATGGAGAAGTGTGCC
CTCATGGAAGCCCTGGTTCTCATTAGCAACCAATTTAAGAACTACGAGCG
TCAGAAGGTGTTCCTAGAGGAGCTGATGGCACCAGTGGCCAGCATCTGGC
TTTCTCAAGACATGCACAGAGTGCTGTCAGATGTTGATGCTTTCATTGCG
TATGTGGGTACAGATCAGAAGAGCTGTGACCCAGGCCTGGAGGATCCGTG
TGGCTTAAACCGTGCACGAATGAGCTTTTGTGTATACAGCATTCTGGGTG
TGGTGAAACGAACTTGCTGGCCCACTGACCTAGAAGAGGCCAAAGCTGGG
GGATTTGTGGTGGGTTATACATCCAGTGGAAATCCAATCTTCCGTAACCC
CTGCACAGAGCAGATTCTGAAACTTCTTGACAATTTGCTTGCGCTTATAA
GAACCCACAATACATTATATGCACCAGAAATGCTAGCCAAAATGGCAGAG
CCTTTCACCAAGGCTCTGGATATGCTTGACGCGGAAAAATCTGCTATATT
AGGATTACCTCAACCTCTCTTGGAACTCAATGACTCTCCTGTCTTCAAAA
CCGTCTTGGAAAGAATGCAGCGTTTCTTCTCTACCCTCTATGAAAACTGT
TTTCATATCCTAGGGAAGGCAGGCCCTTCCATGCAGCAAGACTTCTATAC
TGTGGAGGACCTTGCTACCCAGCTTCTCAGCTCAGCCTTTGTCAACTTGA
ACAATATTCCTGACTACCGACTCAGACCCATGCTTCGTGTCTTTGTAAAG
CCTCTGGTGCTCTTCTGTCCCCCAGAGCACTATGAAGCCCTGGTATCCCC
CATCCTCGGACCTCTTTTCACCTACCTCCATATGAGGCTTTCTCAGAAAT
GGCAAGTTATCAACCAAAGGAGCCTGCTGTGTGGAGAAGATGAGGCTGCA
GATGAAAACCCAGAGTCTCAAGAGATGCTGGAGGAGCAACTGGTGAGGAT
GTTAACCCGAGAAGTCATGGACCTAATCACGGTTTGCTGTGTTTCAAAGA
AGGGTGCTGACCACAGTAGTGCTCCCCCAGCAGATGGAGACGATGAAGAA
ATGATGGCCACAGAGGTCACCCCCTCAGCTATGGCAGAGCTTACAGACCT
GGGCAAATGTCTGATGAAGCATGAGGATGTTTGTACAGCGCTATTAATTA
CAGCCTTCAATTCCCTGGCCTGGAAAGATACTCTGTCCTGCCAGAGGACA
ACCTCACAGCTCTGCTGGCCTCTCCTCAAACAAGTGCTGTCAGGGACACT
GCTCGCAGATGCAGTTACGTGGCTTTTCACCAGTGTGCTGAAAGGCTTAC
AGATGCACGGGCAGCACGACGGGTGCATGGCTTCCCTGGTCCATCTGGCC
TTCCAGATATATGAGGCACTGCGCCCCAGGTACCTGGAGATAAGAGCTGT
AATGGAGCAAATCCCTGAAATACAGAAGGACTCACTGGACCAGTTTGACT
GCAAGCTTTTAAACCCCTCCCTGCAGAAAGTGGCTGACAAGCGCCGAAAG
GACCAATTCAAACGCCTCATTGCTGGTTGCATTGGGAAACCCTTGGGAGA
GCAGTTCCGAAAAGAAGTTCACATTAAGAATCTTCCCTCACTTTTCAAAA
AAACAAAGCCAATGCTGGAGACGGAGGTGCTGGACAATGATGGGGGTGGC
CTGGCCACCATCTTTGAACCCTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMAMDQVNALCEQLVKAVTVMMDPNSTQ
RYRLEALKFCEEFKEKCPICVPCGLRLAEKTQVAIVRHFGLQILEHVVKF
RWNGMSRLEKVYLKNSVMELIANGTLNILEEENHIKDALSRIVVEMIKRE
WPQHWPDMLIELDTLSKQGETQTELVMFILLRLAEDVVTFQTLPPQRRRD
IQQTLTQNMERIFSFLLNTLQENVNKYQQVKTDTSQESKAQANCRVGVAA
LNTLAGYIDWVSMSHITAENCKLLEILCLLLNEQELQLGAAECLLIAVSR
KGKLEDRKPLMVLFGDVAMHYILSAAQTADGGGLVEKHYVFLKRLCQVLC
ALGNQLCALLGADSDVETPSNFGKYLESFLAFTTHPSQFLRSSTQMTWGA
LFRHEILSRDPLLLAIIPKYLRASMTNLVKMGFPSKTDSPSCEYSRFDFD
SDEDFNAFFNSSRAQQGEVMRLACRLDPKTSFQMAGEWLKYQLSTFLDAG
SVNSCSAVGTGEGSLCSVFSPSFVQWEAMTLFLESVITQMFRTLNREEIP
VNDGIELLQMVLNFDTKDPLILSCVLTNVSALFPFVTYRPEFLPQVFSKL
FSSVTFETVEESKAPRTRAVRNVRRHACSSIIKMCRDYPQLVLPNFDMLY
NHVKQLLSNELLLTQMEKCALMEALVLISNQFKNYERQKVFLEELMAPVA
SIWLSQDMHRVLSDVDAFIAYVGTDQKSCDPGLEDPCGLNRARMSFCVYS
ILGVVKRTCWPTDLEEAKAGGFVVGYTSSGNPIFRNPCTEQILKLLDNLL
ALIRTHNTLYAPEMLAKMAEPFTKALDMLDAEKSAILGLPQPLLELNDSP
VFKTVLERMQRFFSTLYENCFHILGKAGPSMQQDFYTVEDLATQLLSSAF
VNLNNIPDYRLRPMLRVFVKPLVLFCPPEHYEALVSPILGPLFTYLHMRL
SQKWQVINQRSLLCGEDEAADENPESQEMLEEQLVRMLTREVMDLITVCC
VSKKGADHSSAPPADGDDEEMMATEVTPSAMAELTDLGKCLMKHEDVCTA
LLITAFNSLAWKDTLSCQRTTSQLCWPLLKQVLSGTLLADAVTWLFTSVL
KGLQMHGQHDGCMASLVHLAFQIYEALRPRYLEIRAVMEQIPEIQKDSLD
QFDCKLLNPSLQKVADKRRKDQFKRLIAGCIGKPLGEQFRKEVHIKNLPS
LFKKTKPMLETEVLDNDGGGLATIFEP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8516
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; ; Estimated MW of expressed protein : 138671 Daltons