cDNA Clone IKK epsilon

• Plasmid: pFBHTb IKK epsilon
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-IKK epsilon
• Genbank: NP_054721
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCT
GGGGCAGGGGGCCACTGCCAGTGTGTACAAGGCCCGCAACAAGAAATCCG
GAGAGCTGGTTGCTGTGAAGGTCTTCAACACTACCAGCTACCTGCGGCCC
CGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGGAAGCTGAACCACCA
GAACATCGTCAAGCTCTTTGCGGTGGAGGAGACGGGCGGAAGCCGGCAGA
AGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTG
GAGAGCCCTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGT
GCTGCGCTGTGTGGTGGCCGGCATGAACCACCTGCGGGAGAACGGCATTG
TGCATCGCGACATCAAGCCGGGGAACATCATGCGCCTCGTAGGGGAGGAG
GGGCAGAGCATCTACAAGCTGACAGACTTCGGCGCTGCCCGGGAGCTGGA
TGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTGCATC
CCGACATGTATGAGCGGGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTC
GGGGTGACTGTGGATCTCTGGAGCATTGGAGTGACCTTGTACCATGCAGC
CACTGGCAGCCTGCCCTTCATCCCCTTTGGTGGGCCACGGCGGAACAAGG
AGATCATGTACCGGATCACCACGGAGAAGCCGGCTGGGGCCATTGCAGGT
GCCCAGAGGCGGGAGAACGGGCCCCTGGAGTGGAGCTACACCCTCCCCAT
CACCTGCCAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATCCTGG
CCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTC
TTTGCGGAGACCAGTGACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTC
CCTGTCCCAGGCAGTCCTGCACCACATCTATATCCATGCCCACAACACGA
TAGCCATTTTCCAGGAGGCCGTGCACAAGCAGACCAGTGTGGCCCCCCGA
CACCAGGAGTACCTCTTTGAGGGTCACCTCTGTGTCCTCGAGCCCAGCGT
CTCAGCACAGCACATCGCCCACACGACGGCAAGCAGCCCCCTGACCCTCT
TCAGCACAGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCTGGAC
GTCCCCAAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGC
CAAGGGCGTGTTGGGCGCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCC
TGCTGGATGGGCAGGAGCTAATGTTTCGGGGGCTGCACTGGGTCATGGAG
GTGCTCCAGGCCACATGCAGACGGACTCTGGAAGTGGCAAGGACATCCCT
CCTCTACCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTGGCTG
GAACGCCTGAGATCCAGGAACTGAAGGCGGCTGCAGAACTGAGGTCCAGG
CTGCGGACTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCACGGA
GACCCAGGAGAGCCTGAGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGG
ATCAGGTACATGAGGACAGAAGCATCCAGCAGATTCAGTGCTGTTTGGAC
AAGATGAACTTCATCTACAAACAGTTCAAGAAGTCTAGGATGAGGCCAGG
GCTTGGCTACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAATTTCA
GTCATTTAGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAG
AAGTATCAAGCGTCCTTAGTCACACACGGCAAGAGGATGAGGGTGGTGCA
CGAGACCAGGAACCACCTGCGCCTGGTTGGCTGTTCTGTGGCTGCCTGTA
ACACAGAAGCCCAGGGGGTCCAGGAGAGTCTCAGCAAGCTCCTGGAAGAG
CTATCTCACCAGCTCCTTCAGGACCGAGCAAAGGGGGCTCAGGCCTCGCC
GCCTCCCATAGCTCCTTACCCCAGCCCTACACGAAAGGACCTGCTTCTCC
ACATGCAAGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTG
GACAACAACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACCTCCTGA
TGTCTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMQSTANYLWHTDDLLGQGATAS
VYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFA
VEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAG
MNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVS
VYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFI
PFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLG
LQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLH
HIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAH
TTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAG
YQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSL
GTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSS
LNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQ
IHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLR
LVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYP
SPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8460
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; ; Estimated MW of expressed protein : 83830 Daltons