cDNA Clone PRAS40

• Plasmid: pGFP TAPP PRAS40 T246A
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP-PRAS40 T246A
• Genbank: BC007416
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCATGGCGTCGGGGCGCCCCGAGGAGCTGTGGGAGGCCGTGGTGGG
GGCCGCCGAGCGCTTCCGGGCCCGGACTGGCACGGAGCTGGTGCTGCTGA
CCGCGGCCCCGCCGCCACCACCCCGCCCGGGCCCCTGTGCCTATCCTGCC
CATGGTCGAGGAGCCCTGGCGGAGGCAGCGCGCCGTTGCCTCCACGACAT
CGCACTGGCCCACAGGGCTGCCACTGCTGCTCGGCCTCCTGCGCCCCCAC
CAGCACCACAGCCACCCAGTCCCACACCCAGCCCACCCCGGCCTACCCTG
GCCAGAGAGGACAACGAGGAGGACGAGGATGAGCCCACAGAGACAGAGAC
CTCCGGGGAGCAGCTGGGCATTAGTGATAATGGAGGGCTCTTTGTGATGG
ATGAGGACGCCACCCTCCAGGACCTTCCCCCCTTCTGTGAGTCAGACCCC
GAGAGTACAGATGATGGCAGCCTGAGCGAGGAGACCCCCGCCGGCCCCCC
CACCTGCTCAGTGCCCCCAGCCTCAGCCCTACCCACACAGCAGTACGCCA
AGTCCCTGCCTGTGTCTGTGCCCGTCTGGGGCTTCAAGGAGAAGAGGACA
GAGGCGCGGTCATCAGATGAGGAGAATGGGCCGCCCTCTTCGCCCGACCT
GGACCGCATCGCGGCGAGCATGCGCGCGCTGGTGCTGCGAGAGGCCGAGG
ACACCCAGGTCTTCGGGGACCTGCCACGGCCGCGGCTTAACGCCAGCGAC
TTCCAGAAGCTGAAGCGGAAATATTGAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMAS
GRPEELWEAVVGAAERFRARTGTELVLLTAAPPPPPRPGPCAYPAHGRGA
LAEAARRCLHDIALAHRAATAARPPAPPPAPQPPSPTPSPPRPTLAREDN
EEDEDEPTETETSGEQLGISDNGGLFVMDEDATLQDLPPFCESDPESTDD
GSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSS
DEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNASDFQKLK
RKY*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8424
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP/TAPP tag for mammalian expression. ; ; Estimated MW of expressed protein : 77460 Daltons