cDNA Clone TAK1-S

• Plasmid: pFastbac HTb TAK1 31-303 TAB1 468-504
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-TAK1 31-303 TAB1 468-504
• Genbank: pFBHTb
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATCGACTACAAGGAGATCGAGGTGGAAGAGGTTGTTGGAAGAGG
AGCCTTTGGAGTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCTA
TTAAACAAATAGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTT
CGGCAGTTATCCCGTGTGAACCATCCTAATATTGTAAAGCTTTATGGAGC
CTGCTTGAATCCAGTGTGTCTTGTGATGGAATATGCTGAAGGGGGCTCTT
TATATAATGTGCTGCATGGTGCTGAACCATTGCCATATTATACTGCTGCC
CACGCAATGAGTTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTATCTTCA
CAGCATGCAACCCAAAGCGCTAATTCACAGGGACCTGAAACCACCAAACT
TACTGCTGGTTGCAGGGGGGACAGTTCTAAAAATTTGTGATTTTGGTACA
GCCTGTGACATTCAGACACACATGACCAATAACAAGGGGAGTGCTGCTTG
GATGGCACCTGAAGTTTTTGAAGGTAGTAATTACAGTGAAAAATGTGACG
TCTTCAGCTGGGGTATTATTCTTTGGGAAGTGATAACGCGTCGGAAACCC
TTTGATGAGATTGGTGGCCCAGCTTTCCGAATCATGTGGGCTGTTCATAA
TGGTACTCGACCACCACTGATAAAAAATTTACCTAAGCCCATTGAGAGCC
TGATGACTCGTTGTTGGTCTAAAGATCCTTCCCAGCGCCCTTCAATGGAG
GAAATTGTGAAAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCAGA
TGAGCCATTACAGTATCCTTGTCAGCACTCGCTCCCGCCTGGCGAGGACG
GTCGTGTTGAGCCCTATGTGGACTTTGCTGAGTTTTACCGCCTCTGGAGC
GTGGACCATGGCGAGCAGAGCGTGGTGACAGCACCGTAGGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSIDYKEIEVEEVVGRGAFGVVCK
AKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCL
VMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKAL
IHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFE
GSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLI
KNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPC
QHSLPPGEDGRVEPYVDFAEFYRLWSVDHGEQSVVTAP*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8420
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression.; ; Estimated MW of expressed protein : 38322 Daltons