cDNA Clone TPL2

• Synonyms: MAP3K8, COT, EST, ESTF, FLJ10486, MEKK8, TPL2, Tpl-2, c-COT, OTTHUMP00000019392, OTTHUMP00000019393, Ewing sarcoma transformant, cot (cancer Osaka thyroid) oncogene, proto-oncogene c-Cot, proto-oncogene serine/threoine protein kinase, tumor progression locus 2
• Plasmid: pCMV-FLAG TPL2 30-397 S62D T290D
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-TPL2 30-397 S62D T290D
• Genbank: P41279
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGAAAATCTTTATGCAAGTGAAGAGCCAGCAGTTTATGAACC
CAGTCTAATGACCATGTGTCAAGACAGTAATCAAAACGATGAGCGTTCTA
AGGATCTGCTGCTTAGTGGCCAAGAGGTACCATGGTTGTCATCAGTCAGA
TATGGAACTGTGGAGGATTTGCTTGCTTTTGCAAACCATATATCCAACAC
TGCAAAGCATTTTTATGGACAACGACCACAGGAATCTGGAATTTTATTAA
ACATGGTCATCACTCCCCAAAATGGACGTTACCAAATAGATTCCGATGTT
CTCCTGATCCCCTGGAAGCTGACTTACAGGAATATTGGTTCTGATTTTAT
TCCTCGGGGCGCCTTTGGAAAGGTATACTTGGCACAAGATATAAAGACGA
AGAAAAGAATGGCGTGTAAACTGATCCCTGTAGATCAATTTAAGCCATCT
GATGTGGAAATCCAGGCTTGCTTCCGGCACGAGAACATCGCAGAGCTGTA
TGGCGCAGTCCTGTGGGGTGAAACTGTCCATCTCTTTATGGAAGCAGGCG
AGGGAGGGTCTGTTCTGGAGAAACTGGAGAGCTGTGGACCAATGAGAGAA
TTTGAAATTATTTGGGTGACAAAGCATGTTCTCAAGGGACTTGATTTTCT
ACACTCAAAGAAAGTGATCCATCATGATATTAAACCTAGCAACATTGTTT
TCATGTCCACAAAAGCTGTTTTGGTGGATTTTGGCCTAAGTGTTCAAATG
ACCGAAGATGTCTATTTTCCTAAGGACCTCCGAGGAGACGAGATTTACAT
GAGCCCAGAGGTCATCCTGTGCAGGGGCCATTCAACCAAAGCAGACATCT
ACAGCCTGGGGGCCACGCTCATCCACATGCAGACGGGCACCCCACCCTGG
GTGAAGCGCTACCCTCGCTCAGCCTATCCCTCCTACCTGTACATAATCCA
CAAGCAAGCACCTCCACTGGAAGACATTGCAGATGACTGCAGTCCAGGGA
TGAGAGAGCTGATAGAAGCTTCCCTGGAGAGAAACCCCAATCACCGCCCA
AGAGCCGCAGACCTACTAAAACATGAGGCCCTGAACCCGCCCAGAGAGGA
TCAGCCACGCTAAGAATTCCGCAAGGGCGAATTCTGCAGATATCCATCAC
ACTGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMENLYASEEPAVYEPSLMTMCQDSNQNDERSKDLLLSGQ
EVPWLSSVRYGTVEDLLAFANHISNTAKHFYGQRPQESGILLNMVITPQN
GRYQIDSDVLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKL
IPVDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEK
LESCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVL
VDFGLSVQMTEDVYFPKDLRGDEIYMSPEVILCRGHSTKADIYSLGATLI
HMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEAS
LERNPNHRPRAADLLKHEALNPPREDQPR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8395
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal FLAG tag.; ; Estimated MW of expressed protein : 43115 Daltons