cDNA Clone TAK1-S

• Plasmid: pGFP TAPP TAK1 D175A
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP-TAK1 D175A
• Genbank: NP_003179
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCATGTCTACAGCCTCTGCCGCCTCCTCCTCCTCCTCGTCTTCGGC
CGGTGAGATGATCGAAGCCCCTTCCCAGGTCCTCAACTTTGAAGAGATCG
ACTACAAGGAGATCGAGGTGGAAGAGGTTGTTGGAAGAGGAGCCTTTGGA
GTTGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTTGCTATTAAACAAAT
AGAAAGTGAATCTGAGAGGAAAGCGTTTATTGTAGAGCTTCGGCAGTTAT
CCCGTGTGAACCATCCTAATATTGTAAAGCTTTATGGAGCCTGCTTGAAT
CCAGTGTGTCTTGTGATGGAATATGCTGAAGGGGGCTCTTTATATAATGT
GCTGCATGGTGCTGAACCATTGCCATATTATACTGCTGCCCACGCAATGA
GTTGGTGTTTACAGTGTTCCCAAGGAGTGGCTTATCTTCACAGCATGCAA
CCCAAAGCGCTAATTCACAGGGACCTGAAACCACCAAACTTACTGCTGGT
TGCAGGGGGGACAGTTCTAAAAATTTGTGCTTTTGGTACAGCCTGTGACA
TTCAGACACACATGACCAATAACAAGGGGAGTGCTGCTTGGATGGCACCT
GAAGTTTTTGAAGGTAGTAATTACAGTGAAAAATGTGACGTCTTCAGCTG
GGGTATTATTCTTTGGGAAGTGATAACGCGTCGGAAACCCTTTGATGAGA
TTGGTGGCCCAGCTTTCCGAATCATGTGGGCTGTTCATAATGGTACTCGA
CCACCACTGATAAAAAATTTACCTAAGCCCATTGAGAGCCTGATGACTCG
TTGTTGGTCTAAAGATCCTTCCCAGCGCCCTTCAATGGAGGAAATTGTGA
AAATAATGACTCACTTGATGCGGTACTTTCCAGGAGCAGATGAGCCATTA
CAGTATCCTTGTCAGTATTCAGATGAAGGACAGAGCAACTCTGCCACCAG
TACAGGCTCATTCATGGACATTGCTTCTACAAATACGAGTAACAAAAGTG
ACACTAATATGGAGCAAGTTCCTGCCACAAATGATACTATTAAGCGCTTA
GAATCAAAATTGTTGAAAAATCAGGCAAAGCAACAGAGTGAATCTGGACG
TTTAAGCTTGGGAGCCTCCCGTGGGAGCAGTGTGGAGAGCTTGCCCCCAA
CCTCTGAGGGCAAGAGGATGAGTGCTGACATGTCTGAAATAGAAGCTAGG
ATCGCCGCAACCACAGGCAACGGACAGCCAAGACGTAGATCCATCCAAGA
CTTGACTGTAACTGGAACAGAACCTGGTCAGGTGAGCAGTAGGTCATCCA
GTCCCAGTGTCAGAATGATTACTACCTCAGGACCAACCTCAGAAAAGCCG
ACTCGAAGTCATCCATGGACCCCTGATGATTCCACAGATACCAATGGATC
AGATAACTCCATCCCAATGGCTTATCTTACACTGGATCACCAACTACAGC
CTCTAGCACCGTGCCCAAACTCCAAAGAATCTATGGCAGTGTTTGAACAG
CATTGTAAAATGGCACAAGAATATATGAAAGTTCAAACAGAAATTGCATT
GTTATTACAGAGAAAGCAAGAACTAGTTGCAGAACTGGACCAGGATGAAA
AGGACCAGCAAAATACATCTCGCCTGGTACAGGAACATAAAAAGCTTTTA
GATGAAAACAAAAGCCTTTCTACTTACTACCAGCAATGCAAAAAACAACT
AGAGGTCATCAGAAGTCAGCAGCAGAAACGACAAGGCACTTCATGAGGAT
CC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSMST
ASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKA
KWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLV
MEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALI
HRDLKPPNLLLVAGGTVLKICAFGTACDIQTHMTNNKGSAAWMAPEVFEG
SNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIK
NLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQ
YSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLL
KNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIAATT
GNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPTRSHP
WTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMA
QEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKS
LSTYYQQCKKQLEVIRSQQQKRQGTS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8389
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GFP-TAP for mammalian expression. Kinase dead mutation.