cDNA Clone TGFBR1

• Plasmid: pGEX-4T-TGFBR1 148-503
• Parent Plasmid: pGEX4T1
• Expressed: GST-4T TGFB1 148-503
• Genbank: NP_004603
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCTGCCACAACCGCACTGTCATTCACCATCGAGTGCCAAATGAAGA
GGACCCTTCATTAGATCGCCCTTTTATTTCAGAGGGTACTACGTTGAAAG
ACTTAATTTATGATATGACAACGTCAGGTTCTGGCTCAGGTTTACCATTG
CTTGTTCAGAGAACAATTGCGAGAACTATTGTGTTACAAGAAAGCATTGG
CAAAGGTCGATTTGGAGAAGTTTGGAGAGGAAAGTGGCGGGGAGAAGAAG
TTGCTGTTAAGATATTCTCCTCTAGAGAAGAACGTTCGTGGTTCCGTGAG
GCAGAGATTTATCAAACTGTAATGTTACGTCATGAAAACATCCTGGGATT
TATAGCAGCAGACAATAAAGACAATGGTACTTGGACTCAGCTCTGGTTGG
TGTCAGATTATCATGAGCATGGATCCCTTTTTGATTACTTAAACAGATAC
ACAGTTACTGTGGAAGGAATGATAAAACTTGCTCTGTCCACGGCGAGCGG
TCTTGCCCATCTTCACATGGAGATTGTTGGTACCCAAGGAAAGCCAGCCA
TTGCTCATAGAGATTTGAAATCAAAGAATATCTTGGTAAAGAAGAATGGA
ACTTGCTGTATTGCAGACTTAGGACTGGCAGTAAGACATGATTCAGCCAC
AGATACCATTGATATTGCTCCAAACCACAGAGTGGGAACAAAAAGGTACA
TGGCCCCTGAAGTTCTCGATGATTCCATAAATATGAAACATTTTGAATCC
TTCAAACGTGCTGACATCTATGCAATGGGCTTAGTATTCTGGGAAATTGC
TCGACGATGTTCCATTGGTGGAATTCATGAAGATTACCAACTGCCTTATT
ATGATCTTGTACCTTCTGACCCATCAGTTGAAGAAATGAGAAAAGTTGTT
TGTGAACAGAAGTTAAGGCCAAATATCCCAAACAGATGGCAGAGCTGTGA
AGCCTTGAGAGTAATGGCTAAAATTATGAGAGAATGTTGGTATGCCAATG
GAGCAGCTAGGCTTACAGCATTGCGGATTAAGAAAACATTATCGCAACTC
AGTCAACAGGAAGGCATCAAAATGTAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPEFCHNRTVIHHRVPNEEDPSLDR
PFISEGTTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGE
VWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNK
DNGTWTQLWLVSDYHEHGSLFDYLNRYTVTVEGMIKLALSTASGLAHLHM
EIVGTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIA
PNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGLVFWEIARRCSIG
GIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKLRPNIPNRWQSCEALRVMA
KIMRECWYANGAARLTALRIKKTLSQLSQQEGIKM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8387
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; ; Estimated MW of expressed protein : 67223 Daltons