cDNA Clone SGK2

• Plasmid: pFBHTc SGK2
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-SGK2
• Genbank: AAF12757
• Tag: 6His

Sequence of Insert

GGATCCAGCTGCCTGATCATTGCTACAGAATGAACTCTAGCCCAGCTGGG
ACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATCAACCTGGGGCC
TTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTCA
TCGGCAAAGGGAACTACGGGAAGGTCCTACTGGCCAAGCGCAAGTCTGAT
GGGGCGTTCTATGCAGTGAAGGTACTACAGAAAAAGTCCATCTTAAAGAA
GAAAGAGCAGAGCCACATCATGGCAGAGCGCAGTGTGCTTCTGAAGAACG
TGCGGCACCCCTTCCTCGTGGGCCTGCGCTACTCCTTCCAGACACCTGAG
AAGCTCTACTTCGTGCTCGACTATGTCAACGGGGGAGAGCTCTTCTTCCA
CCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCTG
CTGAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTAC
AGGGATCTGAAACCAGAGAACATTCTCTTGGACTGCCAGGGACACGTGGT
GCTGACGGATTTTGGCCTCTGCAAGGAAGGTGTAGAGCCTGAAGACACCA
CATCCACATTCTGTGGTACCCCTGAGTACTTGGCACCTGAAGTGCTTCGG
AAAGAGCCTTATGATCGAGCAGTGGACTGGTGGTGCTTGGGGGCAGTCCT
CTACGAGATGCTCCATGGCCTGCCGCCCTTCTACAGCCAAGATGTATCCC
AGATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCGG
ACAGTGGCCGCCTGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAG
GCAGCGGCTGGGCTCCAAAGCAGACTTTCTTGAGATTAAGAACCATGTAT
TCTTCAGCCCCATAAACTGGGATGACCTGTACCACAAGAGGCTAACTCCA
CCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAGCATTTTGACCC
AGAGTTCACCCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGACA
CTGTGGCCAGCAGCTCTGGGGCCTCAAGTGCATTCCTGGGATTTTCTTAT
GCGCCAGAGGATGATGACATCTTGGATTGCTAGGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIQLPDHCYRMNSSPAGTPSPQPS
RANGNINLGPSANPNAQPTDFDFLKVIGKGNYGKVLLAKRKSDGAFYAVK
VLQKKSILKKKEQSHIMAERSVLLKNVRHPFLVGLRYSFQTPEKLYFVLD
YVNGGELFFHLQRERRFLEPRARFYAAEVASAIGYLHSLNIIYRDLKPEN
ILLDCQGHVVLTDFGLCKEGVEPEDTTSTFCGTPEYLAPEVLRKEPYDRA
VDWWCLGAVLYEMLHGLPPFYSQDVSQMYENILHQPLQIPGGRTVAACDL
LQSLLHKDQRQRLGSKADFLEIKNHVFFSPINWDDLYHKRLTPPFNPNVT
GPADLKHFDPEFTQEAVSKSIGCTPDTVASSSGASSAFLGFSYAPEDDDI
LDC*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8301
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression.; Estimated MW of expressed protein : 45584 Daltons