cDNA Clone 14-3-3 eta

• Synonyms: YWHAH
• Plasmid: pET28a 14-3-3 sigma
• Parent Plasmid: pET28a original
• Expressed: 6His-14-3-3 sigma
• Genbank: NP_006133.1
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGAGAGAGCCAGTCTGATCCAGAAGGCCAAGCTGGCAGAGCA
GGCCGAACGCTATGAGGACATGGCAGCCTTCATGAAAGGCGCCGTGGAGA
AGGGCGAGGAGCTCTCCTGCGAAGAGCGAAACCTGCTCTCAGTAGCCTAT
AAGAACGTGGTGGGCGGCCAGAGGGCTGCCTGGAGGGTGCTGTCCAGTAT
TGAGCAGAAAAGCAACGAGGAGGGCTCGGAGGAGAAGGGGCCCGAGGTGC
GTGAGTACCGGGAGAAGGTGGAGACTGAGCTCCAGGGCGTGTGCGACACC
GTGCTGGGCCTGCTGGACAGCCACCTCATCAAGGAGGCCGGGGACGCCGA
GAGCCGGGTCTTCTACCTGAAGATGAAGGGTGACTACTACCGCTACCTGG
CCGAGGTGGCCACCGGTGACGACAAGAAGCGCATCATTGACTCAGCCCGG
TCAGCCTACCAGGAGGCCATGGACATCAGCAAGAAGGAGATGCCGCCCAC
CAACCCCATCCGCCTGGGCCTGGCCCTGAACTTTTCCGTCTTCCACTACG
AGATCGCCAACAGCCCCGAGGAGGCCATCTCTCTGGCCAAGACCACTTTC
GACGAGGCCATGGCTGATCTGCACACCCTCAGCGAGGACTCCTACAAAGA
CAGCACCCTCATCATGCAGCTGCTGCGAGACAACCTGACACTGTGGACGG
CCGACAACGCCGGGGAAGAGGGGGGCGAGGCTCCCCAGGAGCCCCAGAGC
TGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMERASLIQKAKLAEQA
ERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIE
QKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAES
RVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTN
PIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDS
TLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU8264
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for bacterial expression.; Estimated MW of expressed protein : 31458 Daltons