cDNA Clone PP4R3

• Plasmid: pEGFP-C1-PP4R3 delta 1-101
• Parent Plasmid: pEGFPC1
• Expressed: GFP-PP4R3 delta 1-101
• Genbank: AAH72409
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCGGAAAGGACCCTTCCGTGGACATCACTCAGGACCTTGTGGATGA
ATCTGAAGAGGAGCGTTTTGATGATATGTCATCGCCAGGCTTAGAATTGC
CATCTTGTGAATTAAGTCGCCTTGAAGAAATTGCAGAACTTGTGGCATCA
TCTTTACCTTCACCTCTTCGTCGTGAAAAACTTGCACTGGCACTAGAAAA
TGAGGGTTATATTAAAAAGCTCCTGGAGCTTTTTCATGTGTGTGAAGATT
TGGAAAATATTGAAGGACTGCACCACTTGTATGAAATTATCAAAGGCATC
TTTCTCTTGAATCGAACTGCTCTTTTTGAAGTTATGTTCTCTGAAGAATG
TATAATGGACGTCATTGGATGTTTAGAATATGATCCTGCTTTATCACAAC
CACGAAAACACAGGGAATTTCTAACAAAAACAGCCAAGTTTAAAGAAGTG
ATTCCCATATCAGATCCTGAGCTGAAACAAAAAATTCATCAGACATACAG
AGTTCAGTATATACAAGATATGGTTCTACCAACTCCTTCGGTCTTTGAAG
AAAACATGTTATCAACACTTCACTCTTTTATCTTTTTCAATAAGGTAGAG
ATTGTTGGCATGTTGCAGGAAGATGAAAAATTTCTGACAGATTTGTTTGC
ACAACTAACAGATGAAGCAACAGATGAGGAAAAAAGACAGGAATTGGTTA
ACTTTTTAAAAGAATTTTGTGCGTTTTCCCAAACGCTACAGCCTCAAAAC
AGAGATGCTTTTTTCAAGACTTTGTCAAACATGGGCATATTACCAGCTTT
AGAAGTCATCCTTGGCATGGATGATACACAGGTGCGAAGTGCTGCTACTG
ATATATTCTCATACTTGGTTGAATATAATCCATCCATGGTACGAGAGTTT
GTCATGCAGGAGGCACAACAGAATGATGATGTAAGTAAGAAGTTAACAGA
GCAAAAAATAACCAGCAAGGATATTTTGCTCATCAACCTCATTATAGAAC
ATATGATTTGTGATACAGATCCTGAACTTGGAGGAGCAGTCCAGCTTATG
GGCCTGCTTCGAACTTTAGTTGACCCAGAGAACATGCTAGCCACTGCCAA
TAAAACAGAAAAGACTGAATTTCTGGGTTTCTTCTACAAGCACTGTATGC
ATGTTCTCACTGCTCCTTTACTAGCAAATACAACAGAAGACAAACCTAGT
AAAGATGATTTTCAGACTGCCCAACTATTGGCACTTGTATTGGAATTGTT
AACATTTTGTGTGGAGCACCATACCTACCACATAAAGAACTACATTATTA
ATAAGGATATCCTCCGGAGAGTGCTAGTTCTTATGGCCTCGAAGCATGCT
TTCTTGGCATTATGTGCCCTTCGTTTTAAAAGAAAGATTATTGGATTAAA
AGATGAGTTTTACAACCGCTACATAATGAAAAGTTTTTTGTTTGAACCAG
TAGTGAAAGCATTTCTCAACAATGGATCCCGCTACAATCTGATGAACTCT
GCCATAATAGAGATGTTTGAATTTATTAGAGTGGAAGATATAAAATCATT
AACTGCTCATGTAATTGAAAATTACTGGAAAGCACTGGAAGATGTAGATT
ATGTACAGACATTTAAAGGATTAAAACTGAGATTTGAACAACAAAGAGAA
AGGCAAGATAATCCCAAACTTGACAGTATGCGTTCCATTTTGAGGAATCA
CAGATATCGAAGAGATGCCAGAACACTAGAAGATGAAGAAGAGATGTGGT
TTAACACAGATGAAGATGACATGGAAGATGGAGAAGCTGTAGTGTCTCCA
TCTGACAAAACTAAAAATGATGATGATATTATGGATCCAATAAGTAAATT
CATGGAAAGGAAGAAATTAAAAGAAAGTGAGGAAAAGGAAGTGCTTCTGA
AAACAAACCTTTCTGGACGGCAGAGCCCAAGTTTCAAGCTTTCCCTGTCC
AGTGGAACGAAGACTAACCTCACCAGCCAGTCATCTACAACAAATCTGCC
TGGTTCTCCGGGATCACCTGGATCCCCAGGATCTCCAGGCTCTCCTGGAT
CCGTACCTAAAAATACATCTCAGACGGCAGCTATTACTACAAAGGGAGGC
CTCGTGGGTCTGGTAGATTATCCTGATGATGATGAAGATGATGATGAGGA
TGAAGATAAGGAAGATACGTTACCATTGTCAAAGAAAGCAAAATTTGATT
CATAAGTCGAC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFGKDPSVDITQDLVDESEEERFDDMSSPGLELPSCELSRLEEIAELVAS
SLPSPLRREKLALALENEGYIKKLLELFHVCEDLENIEGLHHLYEIIKGI
FLLNRTALFEVMFSEECIMDVIGCLEYDPALSQPRKHREFLTKTAKFKEV
IPISDPELKQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVE
IVGMLQEDEKFLTDLFAQLTDEATDEEKRQELVNFLKEFCAFSQTLQPQN
RDAFFKTLSNMGILPALEVILGMDDTQVRSAATDIFSYLVEYNPSMVREF
VMQEAQQNDDVSKKLTEQKITSKDILLINLIIEHMICDTDPELGGAVQLM
GLLRTLVDPENMLATANKTEKTEFLGFFYKHCMHVLTAPLLANTTEDKPS
KDDFQTAQLLALVLELLTFCVEHHTYHIKNYIINKDILRRVLVLMASKHA
FLALCALRFKRKIIGLKDEFYNRYIMKSFLFEPVVKAFLNNGSRYNLMNS
AIIEMFEFIRVEDIKSLTAHVIENYWKALEDVDYVQTFKGLKLRFEQQRE
RQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSP
SDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLS
SGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGG
LVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU8241
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GFP for mammalian expression.; Estimated MW of expressed protein : 112028 Daltons