cDNA Clone REV1

• Plasmid: pCMV-HA-1 REV1
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-REV1
• Genbank: AAF18986
• Tag: HA
• Species: Human

Sequence of Insert

GTCGACCATGAGGCGAGGTGGATGGAGGAAGCGAGCTGAAAATGATGGCT
GGGAAACATGGGGTGGGTATATGGCTGCCAAGGTCCAGAAATTGGAGGAA
CAGTTTCGATCAGATGCTGCTATGCGGAAGGATGGGACTTCATCTACAAT
TTTTAGTGGAGTTGCCATCTATGTTAATGGATACACAGATCCTTCCGCTG
AGGAATTGAGAAAACTAATGATGTTGCATGGAGGTCAATACCATGTATAT
TATTCCAGATCTAAAACAACACATATTATTGCCACAAATCTTCCCAATGC
CAAAATTAAAGAATTAAAGGGGGAAAAAGTAATTCGACCAGAATGGATTG
TGGAAAGCATCAAAGCTGGACGACTCCTCTCCTACATTCCATATCAGCTG
TACACCAAGCAGTCCAGTGTGCAGAAAGGTCTCAGCTTTAATCCTGTATG
CAGACCTGAGGATCCTCTGCCAGGTCCAAGCAATATAGCCAAACAGCTCA
ACAACAGGGTAAATCACATCGTTAAGAAGATTGAAACGGAAAATGAAGTC
AAAGTCAATGGCATGAACAGTTGGAATGAAGAAGATGAAAATAATGATTT
TAGTTTTGTGGATCTGGAGCAGACCTCTCCGGGAAGGAAACAGAATGGAA
TTCCGCATCCCAGAGGGAGCACTGCCATTTTTAATGGACACACTCCTAGC
TCTAATGGTGCCTTAAAGACACAGGATTGCTTGGTGCCCATGGTCAACAG
TGTTGCCAGCAGGCTTTCTCCAGCCTTTTCCCAGGAGGAGGATAAGGCTG
AGAAGAGCAGCACTGATTTCAGAGACTGCACTCTGCAGCAGTTGCAGCAA
AGCACCAGAAACACAGATGCTTTGCGGAATCCACACAGAACTAATTCTTT
CTCATTATCACCTTTGCACAGTAACACTAAAATCAATGGTGCTCACCACT
CCACTGTTCAGGGGCCTTCAAGCACAAAAAGCACTTCTTCAGTATCTACG
TTTAGCAAGGCAGCACCTTCAGTGCCATCCAAACCTTCAGACTGCAATTT
TATTTCAAACTTCTATTCTCATTCAAGACTGCATCACATATCAATGTGGA
AGTGTGAATTGACTGAGTTTGTCAATACCCTACAAAGACAAAGTAATGGT
ATCTTTCCAGGAAGGGAAAAGTTAAAAAAAATGAAAACAGGCAGGTCTGC
ACTTGTTGTAACTGACACAGGAGATATGTCAGTATTGAATTCTCCCAGAC
ATCAGAGCTGTATAATGCATGTTGATATGGATTGCTTCTTTGTATCAGTG
GGTATACGAAATAGACCAGATCTCAAAGGAAAACCAGTGGCTGTTACAAG
TAACAGAGGCACAGGAAGGGCACCTTTACGTCCTGGCGCTAACCCCCAGC
TGGAGTGGCAGTATTACCAGAATAAAATCCTGAAAGGCAAAGCAGCAGAT
ATACCAGATTCATCATTGTGGGAGAATCCAGATTCTGCGCAAGCAAATGG
AATTGATTCTGTTTTGTCAAGGGCTGAAATTGCATCTTGTAGTTATGAGG
CCAGGCAACTTGGCATTAAGAACGGAATGTTTTTTGGGCATGCTAAACAA
CTATGTCCTAATCTTCAAGCTGTTCCATACGATTTTCATGCATATAAGGA
AGTCGCACAAACATTGTATGAAACATTGGCAAGCTACACTCATAACATTG
AAGCTGTCAGTTGTGATGAAGCGCTGGTAGACATTACCGAAATCCTTGCA
GAGACCAAACTTACTCCTGATGAATTTGCAAATGCTGTTCGTATGGAAAT
CAAAGACCAGACGAAATGTGCTGCCTCTGTTGGAATTGGTTCTAATATTC
TCCTGGCTAGAATGGCAACTAGAAAAGCAAAACCAGATGGGCAGTACCAC
CTAAAACCAGAAGAAGTAGATGATTTTATCAGAGGCCAGCTAGTGACCAA
TCTACCAGGAGTTGGACATTCAATGGAATCTAAGTTGGCATCTTTGGGAA
TTAAAACTTGTGGAGACTTGCAGTATATGACCATGGCAAAACTCCAAAAA
GAATTTGGTCCCAAAACAGGTCAGATGCTTTATAGGTTCTGCCGTGGCTT
GGATGATAGACCAGTTCGAACTGAAAAGGAAAGAAAATCTGTTTCAGCTG
AGATCAACTATGGAATAAGGTTTACTCAGCCAAAAGAGGCAGAAGCTTTT
CTTCTGAGTCTTTCAGAAGAAATTCAAAGAAGACTAGAAGCCACTGGCAT
GAAGGGTAAACGTCTAACTCTCAAAATCATGGTACGAAAGCCTGGGGCTC
CTGTAGAAACTGCAAAATTTGGAGGCCATGGAATTTGTGATAACATTGCC
AGGACTGTAACTCTTGACCAGGCAACAGATAATGCAAAAATAATTGGAAA
GGCGATGCTAAACATGTTTCATACAATGAAACTAAATATATCAGATATGA
GAGGGGTTGGGATTCACGTGAATCAGTTGGTTCCAACTAATCTGAACCCT
TCCACATGTCCCAGTCGCCCATCAGTTCAGTCAAGCCACTTTCCTAGTGG
GTCATACTCTGTCCGTGATGTCTTCCAAGTTCAGAAAGCTAAGAAATCCA
CCGAAGAGGAGCACAAAGAAGTATTTCGGGCTGCTGTGGATCTGGAAATA
TCATCTGCTTCTAGAACTTGCACTTTCTTGCCACCTTTTCCTGCACATCT
GCCGACCAGTCCTGATACTAACAAGGCTGAGTCTTCAGGGAAATGGAATG
GTCTACATACTCCTGTCAGTGTGCAGTCGAGACTTAACCTGAGTATAGAG
GTCCCGTCACCTTCCCAGCTGGATCAGTCTGTTTTAGAAGCACTTCCACC
TGATCTCCGGGAACAAGTAGAGCAAGTCTGTGCTGTCCAGCAAGCAGAGT
CACATGGCGACAAAAAGAAAGAACCAGTAAATGGCTGTAATACAGGAATT
TTGCCACAACCAGTTGGGACAGTCTTGTTGCAAATACCAGAACCTCAAGA
ATCGAACAGTGACGCAGGAATAAATTTAATAGCCCTTCCAGCATTTTCAC
AGGTGGACCCTGAGGTATTTGCTGCCCTTCCTGCTGAACTTCAGAGGGAG
CTGAAAGCAGCGTATGATCAAAGACAAAGGCAGGGCGAGAACAGCACTCA
CCAGCAGTCAGCCAGCGCATCTGTGCCAAAGAATCCTTTACTTCATCTAA
AGGCAGCAGTGAAAGAAAAGAAAAGAAACAAGAAGAAAAAAACCATTGGT
TCACCAAAAAGGATTCAGAGTCCTTTGAATAACAAGCTGCTTAACAGTCC
TGCAAAAACTCTGCCAGGGGCCTGTGGCAGTCCCCAGAAGTTAATTGATG
GGTTTCTAAAACATGAAGGACCTCCTGCAGAGAAACCCCTGGAAGAACTC
TCTGCTTCTACTTCAGGTGTGCCAGGCCTTTCTAGTTTGCAGTCTGACCC
AGCTGGCTGTGTGAGACCTCCAGCACCCAATCTAGCTGGAGCTGTTGAAT
TCAATGATGTGAAGACCTTGCTCAGAGAATGGATAACTACAATTTCAGAT
CCAATGGAAGAAGACATTCTCCAAGTTGTGAAATACTGTACTGATCTAAT
AGAAGAAAAAGATTTGGAAAAACTGGATCTAGTTATAAAATACATGAAAA
GGCTGATGCAGCAATCGGTGGAATCGGTTTGGAATATGGCATTTGACTTT
ATTCTTGACAATGTCCAGGTGGTTTTACAACAAACTTATGGAAGCACATT
AAAAGTTACATAAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTMRRGGWRKRAENDGWETWGGYMAAKVQKLEE
QFRSDAAMRKDGTSSTIFSGVAIYVNGYTDPSAEELRKLMMLHGGQYHVY
YSRSKTTHIIATNLPNAKIKELKGEKVIRPEWIVESIKAGRLLSYIPYQL
YTKQSSVQKGLSFNPVCRPEDPLPGPSNIAKQLNNRVNHIVKKIETENEV
KVNGMNSWNEEDENNDFSFVDLEQTSPGRKQNGIPHPRGSTAIFNGHTPS
SNGALKTQDCLVPMVNSVASRLSPAFSQEEDKAEKSSTDFRDCTLQQLQQ
STRNTDALRNPHRTNSFSLSPLHSNTKINGAHHSTVQGPSSTKSTSSVST
FSKAAPSVPSKPSDCNFISNFYSHSRLHHISMWKCELTEFVNTLQRQSNG
IFPGREKLKKMKTGRSALVVTDTGDMSVLNSPRHQSCIMHVDMDCFFVSV
GIRNRPDLKGKPVAVTSNRGTGRAPLRPGANPQLEWQYYQNKILKGKAAD
IPDSSLWENPDSAQANGIDSVLSRAEIASCSYEARQLGIKNGMFFGHAKQ
LCPNLQAVPYDFHAYKEVAQTLYETLASYTHNIEAVSCDEALVDITEILA
ETKLTPDEFANAVRMEIKDQTKCAASVGIGSNILLARMATRKAKPDGQYH
LKPEEVDDFIRGQLVTNLPGVGHSMESKLASLGIKTCGDLQYMTMAKLQK
EFGPKTGQMLYRFCRGLDDRPVRTEKERKSVSAEINYGIRFTQPKEAEAF
LLSLSEEIQRRLEATGMKGKRLTLKIMVRKPGAPVETAKFGGHGICDNIA
RTVTLDQATDNAKIIGKAMLNMFHTMKLNISDMRGVGIHVNQLVPTNLNP
STCPSRPSVQSSHFPSGSYSVRDVFQVQKAKKSTEEEHKEVFRAAVDLEI
SSASRTCTFLPPFPAHLPTSPDTNKAESSGKWNGLHTPVSVQSRLNLSIE
VPSPSQLDQSVLEALPPDLREQVEQVCAVQQAESHGDKKKEPVNGCNTGI
LPQPVGTVLLQIPEPQESNSDAGINLIALPAFSQVDPEVFAALPAELQRE
LKAAYDQRQRQGENSTHQQSASASVPKNPLLHLKAAVKEKKRNKKKKTIG
SPKRIQSPLNNKLLNSPAKTLPGACGSPQKLIDGFLKHEGPPAEKPLEEL
SASTSGVPGLSSLQSDPAGCVRPPAPNLAGAVEFNDVKTLLREWITTISD
PMEEDILQVVKYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDF
ILDNVQVVLQQTYGSTLKVT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8213
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal HA for mammalian expression. As genbank except Q40R (in clone used as PCR template); ; Estimated MW of expressed protein : 140266 Daltons