cDNA Clone Poldip3

• Plasmid: pFBHTb Poldip3
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-Poldip3
• Genbank: AAH49840
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGGCGGACATCTCCCTGGACGAACTCATCAGGAAGCGCGG
GGCGGCGGCGAAAGGACGGCTTAATGCCAGACCGGGAGTTGGAGGTGTCC
GATCTCGAGTTGGGATCCAGCAAGGCCTTCTCAGCCAGTCAACACGCACA
GCCACCTTCCAGCAGAGATTTGATGCCCGGCAGAAGATTGGCCTCTCAGA
TGCCCGGCTCAAACTGGGAGTCAAGGATGCCCGGGAGAAGCTTTTGCAGA
AAGATGCCCGATTTCGAATCAAAGGGAAAGTGCAGGATGCCAGAGAGATG
TTGAACTCTCGCAAGCAGCAGACCACGGTGCCCCAGAAGCCCCGCCAGGT
TGCTGATGCCCGGGAGAAGATCAGCTTGAAGAGGAGTTCCCCTGCTGCCT
TCATAAACCCACCCATTGGGACAGTGACCCCTGCTCTGAAGCTCACCAAA
ACCATCCAGGTTCCACAGCAGAAAGCCATGGCACCACTTCATCCCCATCC
TGCCGGAATGAGAATCAATGTTGTCAATAACCACCAGGCCAAACAGAATT
TATATGACCTGGATGAAGATGATGATGGTATAGCTTCCGTTCCTACTAAA
CAGATGAAGTTTGCAGCCTCAGGCGGCTTTCTCCACCACATGGCTGGGCT
AAGCAGTTCCAAGCTTTCCATGTCCAAGGCCCTCCCTCTCACCAAAGTGG
TTCAGAATGATGCATACACAGCTCCTGCTCTCCCTTCCTCTATTCGAACA
AAAGCCTTGACCAACATGTCCCGGACACTGGTGAACAAGGAAGAACCCCC
CAAAGAGCTGCCAGCTGCTGAGCCTGTTCTCAGCCCATTGGAAGGCACCA
AGATGACTGTGAATAATCTGCACCCTCGAGTCACTGAGGAGGACATTGTT
GAGCTTTTCTGTGTGTGTGGGGCCCTCAAGCGAGCTCGACTGGTCCATCC
TGGGGTAGCGGAGGTGGTGTTTGTGAAAAAGGACGATGCCATCACCGCAT
ATAAGAAGTACAACAACCGGTGTCTGGACGGGCAGCCGATGAAGTGCAAC
CTTCACATGAATGGGAATGTTATCACCTCAGACCAGCCCATCCTGCTGCG
GCTGAGTGACAGCCCATCAATGAAAAAGGAGAGCGAGCTGCCTCGCAGGG
TGAACTCTGCCTCCTCCTCCAACCCCCCTGCCGAAGTGGACCCTGACACC
ATCCTGAAGGCACTCTTCAAGTCCTCAGGGGCCTCTGTGACCACGCAGCC
CACAGAATTCAAAATCAAGCTTTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAAMADISLD
ELIRKRGAAAKGRLNARPGVGGVRSRVGIQQGLLSQSTRTATFQQRFDAR
QKIGLSDARLKLGVKDAREKLLQKDARFRIKGKVQDAREMLNSRKQQTTV
PQKPRQVADAREKISLKRSSPAAFINPPIGTVTPALKLTKTIQVPQQKAM
APLHPHPAGMRINVVNNHQAKQNLYDLDEDDDGIASVPTKQMKFAASGGF
LHHMAGLSSSKLSMSKALPLTKVVQNDAYTAPALPSSIRTKALTNMSRTL
VNKEEPPKELPAAEPVLSPLEGTKMTVNNLHPRVTEEDIVELFCVCGALK
RARLVHPGVAEVVFVKKDDAITAYKKYNNRCLDGQPMKCNLHMNGNVITS
DQPILLRLSDSPSMKKESELPRRVNSASSSNPPAEVDPDTILKALFKSSG
ASVTTQPTEFKIKL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU8140
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter 6His for baculovirus expression.; Estimated MW of expressed protein : 50899 Daltons