cDNA Clone PIAS1

• Plasmid: pCMV-HA-1 PIAS1
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-PIAS1
• Genbank: BAC35902
• Tag: HA
• Species: Mouse

Sequence of Insert

GGATCCATGGCGGACAGTGCGGAACTAAAGCAAATGGTTATGAGCCTTAG
AGTTTCTGAACTCCAAGTACTGTTGGGCTACGCTGGGAGGAACAAGCACG
GACGCAAACACGAACTTCTTACAAAAGCCCTGCATTTGTTAAAGGCTGGC
TGTAGTCCTGCTGTACAAATGAAAATTAAAGAACTCTACAGGAGGCGGTT
CCCTCAGAAAATTATGACGCCTGCGGACTTGTCTATCCCCAACGTACATT
CAAGTCCTATGCCTCCGACTCTTTCTCCATCCACCATTCCACAGCTCACT
TATGATGGCCACCCTGCATCATCCCCACTACTCCCTGTTTCTCTTCTGGG
ACCCAAACATGAACTGGAACTCCCACATCTCACGTCAGCGCTGCACCCAG
TCCACCCGGACATAAAGCTGCAGAAGCTACCATTCTATGACCTGTTGGAT
GAACTGATCAAGCCCACCAGTCTAGCTTCAGACAACAGCCAGCGCTTTCG
GGAAACCTGTTTTGCATTTGCCTTGACACCACAACAGGTGCAGCAGATCA
GCAGCTCCATGGATATTTCTGGGACCAAATGTGACTTCACAGTGCAGGTC
CAATTAAGGTTTTGTTTATCAGAAACCAGTTGTCCACAAGAAGATCACTT
CCCACCCAACCTTTGTGTAAAAGTGAATACAAAACCTTGCAGCCTTCCAG
GTTACCTTCCACCTACTAAAAACGGTGTGGAACCAAAGCGACCTAGCCGA
CCAATTAATATCACCTCACTTGTCCGATTGTCCACGACAGTACCAAATAC
CATTGTTGTTTCTTGGACTGCAGAAATTGGAAGAACCTATTCCATGGCAG
TATATCTTGTAAAACAGTTGTCCTCAACAGTTCTTCTTCAGAGGTTACGA
GCAAAGGGAATAAGGAATCCGGATCATTCTAGAGCTTTAATTAAAGAGAA
GTTAACTGCAGATCCAGATAGTGAGATAGCTACTACCAGCCTACGGGTTT
CGCTGCTGTGTCCACTTGGGAAAATGCGACTGACAATCCCCTGTCGGGCA
CTTACCTGCTCCCACCTTCAGTGTTTTGATGCAACTCTTTACATTCAAAT
GAATGAGAAAAAACCAACATGGGTTTGTCCTGTCTGTGATAAGAAGGCCC
CATATGAACACCTTATTATTGACGGGTTGTTTATGGAAATTCTAAAGTAC
TGCACAGACTGTGACGAGATACAGTTTAAGGAGGATGGCTCGTGGGCTCC
AATGAGGTCAAAGAAGGAGGTTCAAGAAGTCACTGCCTCCTACAATGGAG
TTGATGGTTGCTTGAGCTCCACATTGGAGCATCAGGTAGCGTCCCACAAC
CAGTCCTCAAATAAAAACAAGAAAGTCGAGGTCATTGACCTAACCATTGA
CAGCTCGTCAGATGAAGAGGAGGAAGAACCCCCTGCCAAGAGGACCTGTC
CTTCCCTGTCTCCTACGTCACCACTAAGTAATAAAGGCATTTTAAGTCTT
CCTCATCAAGCCTCGCCTGTGTCCCGCACCCCAAGCCTTCCTGCTGTAGA
TACAAGCTACATCAACACCTCCCTCATCCAGGACTACAGGCACCCCTTCC
ACATGACGCCTATGCCTTATGACTTACAAGGATTAGATTTCTTTCCTTTC
TTATCAGGAGACAATCAGCATTACAACACCTCCCTGCTAGCCGCAGCTGC
AGCGGCGGTCTCAGATGACCAGGACCTCCTGCACTCCTCCCGGTTTTTCC
CGTATACCTCCTCGCAGATGTTTCTCGACCAGCTAAGTGCAGGAGGGAGC
ACATCTCTGCCAGCCACCAACGGAAGCAGTAGCGGCAGCAACAGCAGCCT
TGTGTCTTCCAACAGTCTGAGAGAGAGCCATGGCCATGGTGTGGCCAGCA
GGAGCAGCGCAGACACAGCGTCCATCTTTGGCATCATACCAGACATTATC
TCATTGGACTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELL
TKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPPT
LSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKL
QKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDIS
GTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTK
NGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRTYSMAVYLVKQL
SSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLG
KMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLII
DGLFMEILKYCTDCDEIQFKEDGSWAPMRSKKEVQEVTASYNGVDGCLSS
TLEHQVASHNQSSNKNKKVEVIDLTIDSSSDEEEEEPPAKRTCPSLSPTS
PLSNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPY
DLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQM
FLDQLSAGGSTSLPATNGSSSGSNSSLVSSNSLRESHGHGVASRSSADTA
SIFGIIPDIISLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8133
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter HA for mammalian expression.; Estimated MW of expressed protein : 73072 Daltons