cDNA Clone DYRK1A (rat)

• Plasmid: pEBG6P-DYRK1A rat
• Parent Plasmid: pEBG6P
• Expressed: GST-DYRK1A rat
• Genbank: X79769
• Tag: GST
• Species: Rat

Sequence of Insert

GGATCCCCGGGAATTCAGACGATGCATACAGGAGGAGAGACTTCAGCATG
CAAACCTTCATCTGTCCGGCTCGCACCGTCATTCTCATTCCATGCTGCTG
GCCTTCAGATGGCTGCACAGATGCCCCATTCACATCAGTACAGTGACCGT
CGCCAGCCAAACATAAGTGACCAGCAGGTTTCTGCCTTATCATATTCTGA
CCAGATTCAGCAACCTCTAACTAACCAGGTGATGCCTGACATTGTCATGT
TACAGAGGCGGATGCCCCAAACCTTCCGTGATCCAGCAACTGCTCCTCTG
AGAAAACTCTCTGTGGACTTGATCAAAACATACAAGCATATTAATGAGGT
TTACTATGCAAAAAAGAAGCGAAGACACCAACAGGGCCAGGGGGACGATT
CTAGTCATAAGAAGGAGCGGAAGGTTTACAACGATGGTTACGATGACGAT
AACTATGATTACATTGTAAAAAACGGAGAAAAGTGGATGGACCGTTATGA
AATCGACTCCTTAATAGGCAAAGGTTCATTTGGACAGGTTGTGAAAGCAT
ATGACAGAGTGGAACAAGAATGGGTCGCCATTAAAATCATCAAGAACAAG
AAGGCGTTTCTGAATCAAGCGCAGATAGAAGTGCGGCTGCTTGAGCTCAT
GAACAAACATGACACTGAGATGAAGTACTACATAGTGCATTTGAAACGCC
ACTTTATGTTTCGGAACCATCTCTGTCTAGTGTTTGAAATGCTGTCCTAT
AATCTCTATGATTTGTTGAGGAACACCAACTTCCGAGGGGTCTCTTTGAA
CCTGACACGAAAGTTTGCACAACAGATGTGCACAGCATTGCTTTTCCTTG
CGACTCCAGAACTTAGTATCATTCACTGTGACTTAAAGCCGGAGAACATC
CTTCTGTGTAACCCCAAACGAAGTGCAATCAAGATTGTTGACTTTGGCAG
CTCTTGTCAGTTGGGGCAGAGGATATACCAGTATATTCAGAGTCGCTTCT
ATCGGTCTCCAGAGGTGCTACTGGGAATGCCTTATGACCTTGCTATCGAC
ATGTGGTCCCTTGGATGTATCTTGGTTGAAATGCACACTGGAGAGCCTCT
GTTCAGTGGTGCCAATGAGGTGGATCAGATGAATAAAATAGTGGAAGTCT
TGGGCATCCCACCTGCTCATATTCTTGACCAAGCACCGAAAGCAAGAAAG
TTCTTTGAGAAGTTGCCTGATGGCACTTGGAGCTTAAAGAAGACCAAAGA
TGGAAAACGGGAGTACAAACCACCAGGAACCCGTAAACTTCATAATATTC
TTGGAGTGGAAACAGGAGGACCTGGCGGGCGGCGTGCTGGGGAGTCAGGT
CACACTGTAGCTGACTACTTGAAGTTCAAAGACCTCATTTTAAGGATGCT
TGATTATGACCCCAAAACTCGGATTCAACCTTATTATGCCCTGCAGCACA
GTTTTTTCAAGAAAACAGCTGATGAAGGTACAAACACAAGCAACAGTGTG
TCCACCAGCCCTGCCATGCATGGACTGTGAGACACATAGTCCCCAGGTGC
GCCAGCAGTTTCCGGCTCCTCTGGGCTGGTCAGGCACTGAAGCTCCTACA
CAAGTCACTGTTGAAACTCATCCTGTTCAAGAAACAACCTTTCATGTAGC
CCCTCAGCAGAATGCATTGCATCATCACCATGCTAGTGGATCC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSPGIQTMHTGGETS
ACKPSSVRLAPSFSFHAAGLQMAAQMPHSHQYSDRRQPNISDQQVSALSY
SDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHIN
EVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDR
YEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLE
LMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVS
LNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDF
GSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGE
PLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWSLKKT
KDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILR
MLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMHGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8132
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminal GST tag encoding DYRK1a aa 1-500.; Estimated MW of expressed protein : 85498 Daltons