cDNA Clone PIAS3

• Plasmid: pEBG6P-PIAS3
• Parent Plasmid: pEBG6P
• Expressed: GST-PIAS3
• Genbank: AAH51252
• Tag: GST
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGGCGGAGCTGGGCGAGTTAAAGCACATGGTGATGAGTTT
CCGAGTGTCTGAGCTCCAGGTGCTCCTCGGCTTCGCTGGCAGGAACAAGA
GTGGGCGGAAACACGAGCTGCTGGCCAAGGCCCTGCACCTCCTCAAGTCT
AGCTGCGCCCCCAGCGTCCAGATGAAGATCAAAGAACTTTATCGCAGGCG
CTTTCCCCGGAAGACCCTGGGGCCTTCTGATCTCTCCTTGCTTTCTTTGC
CCCCTGGCACCTCTCCTGTAGGCTCCCCCGGCCCCCTTGCTCCCATCCCT
CCCACCCTTCTGACCCCTGGCACCTTGCTAGGCCCTAAGCGTGAGGTGGA
CATGCATCCTCCTCTGCCGCAGCCTGTGCACCCCGATGTCACCATGAAGC
CACTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGACCCACCACCCTT
GCGTCCACCTCCAGCCAGAGGTTCGAGGAAGCCCACTTCACCTTCGCGCT
CACTCCCCAGCAGCTGCAGCAGATTCTCACGTCCAGGGAGGTTCTGCCAG
GAGCCAAGTGTGATTACACCATACAAGTGCAGCTCAGATTCTGTCTCTGT
GAGACCAGCTGCCCTCAGGAGGACTATTTCCCCCCTAACCTCTTTGTTAA
GGTTAATGGGAAACTCTGCCCCCTGCCGGGTTACCTCCCTCCAACCAAGA
ATGGAGCTGAGCCCAAGAGGCCCAGCCGTCCGATCAACATCACACCCTTG
GCTCGACTCTCAGCCACTGTCCCCAACACCATCGTAGTTAATTGGTCATC
TGAGTTTGGACGGAATTACTCCTTGTCCGTGTACCTGGTGAGGCAATTGA
CTGCAGGGACCCTTCTACAAAAACTCAGAGCCAAGGGGATCCGGAATCCA
GACCATTCCCGGGCACTGATCAAGGAGAAACTGACTGCTGACCCCGACAG
TGAAGTGGCTACTACAAGTCTCCGGGTGTCACTCATGTGCCCGCTAGGGA
AGATGCGCCTGACTGTCCCGTGTCGTGCCCTCACCTGTGCCCATCTGCAG
AGTTTCGATGCTGCCCTTTATCTACAGATGAATGAGAAGAAGCCGACATG
GACGTGTCCTGTGTGTGACAAGAAGGCTCCCTATGAGTCGCTGATTATTG
ATGGTTTATTCATGGAAATTCTTAATTCCTGTTCGGATTGTGATGAGATC
CAGTTCATGGAAGATGGATCCTGGTGTCCGATGAAACCCAAGAAGGAGGC
ATCAGAGGTTTGCCCCCCGCCAGGGTATGGGCTGGATGGTCTCCAGTACA
GCGCAGTCCAGGAGGGAATTCAGCCAGAGAGTAAGAAGAGGGTCGAAGTC
ATTGACTTGACCATCGAAAGCTCATCAGATGAGGAGGATTTGCCCCCCAC
CAAGAAGCACTGCCCTGTCACCTCAGCGGCCATTCCAGCCCTTCCTGGAA
GCAAAGGAGCCCTGACCTCTGGTCACCAGCCATCCTCGGTGCTGCGGAGC
CCTGCAATGGGCACACTGGGCAGTGACTTCCTGTCTAGTCTCCCGCTACA
TGAGTACCCACCTGCCTTCCCACTGGGGGCTGACATCCAAGGTTTAGATT
TATTTTCTTTCCTTCAGACTGAGAGTCAGCACTACGGCCCTTCAGTTATC
ACTTCGCTAGATGAACAGGACACCCTTGGCCACTTCTTCCAGTACCGGGG
AACCCCTTCCCACTTCCTGGGCCCACTGGCCCCCACACTGGGGAGCTCTC
ACCGCAGCTCCACTCCAGCGCCCCCTCCTGGTCGTGTCAGCAGCATTGTG
GCTCCTGGGAGCTCCTTGAGGGAAGGGCATGGAGGACCCCTGCCTTCAGG
TCCCTCTTTGACTGGCTGTCGGTCAGACGTCATTTCCTTGGACTGAGCGG
CCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMAEL
GELKHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQ
MKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPGPLAPIPPTLLTPG
TLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQR
FEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQE
DYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATV
PNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALI
KEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALY
LQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGS
WCPMKPKKEASEVCPPPGYGLDGLQYSAVQEGIQPESKKRVEVIDLTIES
SSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLG
SDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQD
TLGHFFQYRGTPSHFLGPLAPTLGSSHRSSTPAPPPGRVSSIVAPGSSLR
EGHGGPLPSGPSLTGCRSDVISLD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8099
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; Estimated MW of expressed protein : 96520 Da