cDNA Clone LCB1

• Plasmid: pGEX6P-1-LCB1
• Parent Plasmid: pGEX6P1
• Genbank: BAC40709
• Species: Mouse

Sequence of Insert

GGATCCATGGCGACAGTGGCGGAGCAGTGGGTGCTGGTGGAGATGGTGCA
GGCGCTGTACGAGGCTCCAGCATACCATCTTATTTTGGAAGGAATCCTAA
TACTTTGGATAATCAGACTCGTTTTCTCTAAAACTTACAAGTTGCAGGAG
CGTTCTGATCTTACAGCCAAGGAAAAGGAAGAACTGATTGAAGAGTGGCA
GCCAGAGCCCCTCGTCCCTCCAGTCTCCAAGAACCACCCTGCTCTCAACT
ACAACATCGTGTCCGGCCCTCCAACCCACAACATCGTGGTGAATGGAAAA
GAGTGTGTCAACTTTGCCTCCTTTAACTTCCTTGGGCTGCTGGCCAACCC
TCGAGTTAAGGCCGCAGCTTTTTCATCTTTAAAGAAGTACGGAGTGGGTA
CCTGTGGTCCTCGAGGGTTCTATGGCACATTTGATGTCCATCTGGATTTA
GAAGAGCGCCTGGCAAAGTTTATGAAGACCGAAGAAGCCATCATTTACTC
GTATGGCTTCTCCACCATAGCCAGTGCCATTCCTGCGTACTCTAAGAGAG
GGGACATCATCTTTGTGGACAGTGCGGCCTGCTTTGCTATCCAGAAAGGA
TTACAGGCATCAAGAAGTGACATTAAGTTATTTAAGCATAATGATGTGGC
TGACCTGGAGCGACTGCTAAAAGAACAAGAGATTGAAGATCAAAAGAATC
CTCGCAAGGCCCGTGTGACTCGGCGGTTCATCGTGGTGGAGGGCTTGTAC
ATGAACACTGGAACCATCTGTCCCCTTCCAGAACTGGTTAAATTAAAATA
TAAATATAAAGCAAGAATATTCCTGGAGGAGAGCCTGTCCTTTGGAGTCC
TTGGGGAGCACGGGCGAGGAGTCACCGAGCACTATGGGATCAGTATTGAT
GATATTGACCTTATCAGTGCTAACATGGAGAATGCACTCGCTTCTGTCGG
GGGCTTCTGCTGTGGCCGCTCCTTCGTGGTTGACCATCAGCGGCTCTCCG
GTCAAGGATACTGCTTTTCTGCTTCGTTACCTCCCCTGCTAGCTGCTGCT
GCCATTGAGGCCCTCAACATCATGGAAGAGAATCCAGATATTTTTGCAGT
TTTAAAGAAAAAATGCCAGAACATCCATAAGTCTCTACAAGGTGTTTCGG
GTTTAAAAGTGGTGGGAGAGTCCCTTTCTCCGGCGCTTCATCTCCAGCTG
GAAGAGAGCACGGGCTCTCGGGAGAAAGATGTGAAGCTGCTTCAAGCAAT
CGTAGATCAGTGCATGGATAAGGGCATCGCATTGACTCAGGCACGGTACT
TGGACAAGGAAGAGAAGTGCCTTCCTCCTCCAAGCATCAGGGTTGTGGTC
ACTGTGGAGCAGACGGAGGAAGAGCTACAGAGGGCTGCATCCACCATCAG
GGAGGCGGCCCAGGCTGTGCTGTTGTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATVAEQWVLVEMVQALYE
APAYHLILEGILILWIIRLVFSKTYKLQERSDLTAKEKEELIEEWQPEPL
VPPVSKNHPALNYNIVSGPPTHNIVVNGKECVNFASFNFLGLLANPRVKA
AAFSSLKKYGVGTCGPRGFYGTFDVHLDLEERLAKFMKTEEAIIYSYGFS
TIASAIPAYSKRGDIIFVDSAACFAIQKGLQASRSDIKLFKHNDVADLER
LLKEQEIEDQKNPRKARVTRRFIVVEGLYMNTGTICPLPELVKLKYKYKA
RIFLEESLSFGVLGEHGRGVTEHYGISIDDIDLISANMENALASVGGFCC
GRSFVVDHQRLSGQGYCFSASLPPLLAAAAIEALNIMEENPDIFAVLKKK
CQNIHKSLQGVSGLKVVGESLSPALHLQLEESTGSREKDVKLLQAIVDQC
MDKGIALTQARYLDKEEKCLPPPSIRVVVTVEQTEEELQRAASTIREAAQ
AVLL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8089
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Estimated MW of expressed protein : 79278 Daltons