cDNA Clone LCB2 (mouse)

• Plasmid: pGEX6P-2-LCB2
• Parent Plasmid: pGEX6P1
• Genbank: AAC53310
• Species: Mouse

Sequence of Insert

GAATTCATGCGGCCGGAGCCCGGAGGCTGCTGCTGCCGCCGCCCGATGCG
GGCGAACGGCTGCGTCAAGAACGGGGAAGTGAGGAACGGGTACTTGAGGA
GCAGCACCGCCACCGTCGCGGCTGCCGGCCAGATTCATCATGTAACAGAA
AATGGAGGACTGTACAAAAGACCGTTTAATGAAGCTTTTGAAGAAACACC
CATGCTGGTTGCTGTGCTCACATATGTGGGCTATGGCGTACTCACCCTCT
TTGGATATCTTCGAGATTTCTTGAGGCATTGGAGAATTGAAAAGTGCCAC
CATGCAACAGAAAGAGAAGAACAAAAGGACTTTGTGTCCTTGTATCAGGA
TTTTGAAAACTTCTATACAAGGAACCTCTACATGAGAATCAGAGACAACT
GGAATCGACCTATCTGTAGTGTGCCTGGAGCCAAGGTGGATATCATGGAG
AGAAAATCTCATGACTATAACTGGTCATTCAAGTACACAGGGAATATAAT
TAAAGGTGTAATAAACATGGGTTCCTACAACTATCTTGGATTTGCGAGGA
ACACTGGATCATGTCAGGAAGCAGCTGCTGAAGTCCTCAAGGAGTATGGA
GCAGGGGTGTGCAGCACTCGTCAGGAAATTGGAAACCTGGACAAGCATGA
AGAACTAGAGAAATTGGTAGCAAGGTTCTTAGGTGTGGAAGCTGCTATGA
CCTATGGCATGGGATTTGCAACAAATTCAATGAACATTCCTGCTCTTGTT
GGCAAAGGTTGCCTGATTCTGAGTGATGAGCTGAACCATGCGTCACTGGT
TCTAGGAGCCAGACTGTCAGGAGCAACCATTCGAATCTTCAAACACAACA
ATATGCAAAGCTTAGAGAAGCTTTTAAAAGATGCCATTGTTTATGGTCAG
CCTCGGACAAGAAGACCCTGGAAGAAAATCTTAATCCTTGTGGAAGGCAT
ATATAGTATGGAGGGGTCTATTGTTCGCCTTCCTGAAGTGATTGCTCTCA
AGAAGAAATACAAGGCATACTTGTATCTGGATGAGGCTCACAGCATTGGG
GCTCTTGGCCCTTCAGGGCGAGGCGTGGTAGATTACTTCGGCCTGGATCC
TGAGGATGTAGATGTTATGATGGGAACATTCACAAAGAGCTTCGGTGCTT
CAGGAGGATACATCGGAGGCAAGAAGGAGCTGATAGACTACCTGCGCACA
CATTCTCACAGTGCTGTGTATGCCACGTCGATGTCACCGCCTGTGATGGA
ACAGATTATCACCTCCATGAAGTGCATCATGGGGCAGGATGGCACCAGTC
TTGGCAAAGAATGTATACAGCAGTTGGCTGAGAACACCAGGTATTTCAGG
AGACGCCTGAAGGAAATGGGGTTCATCATCTATGGCAATGAAGACTCCCC
GGTGGTGCCTTTGATGCTCTACATGCCGGCCAAAATTGGCGCCTTTGGAA
GAGAGATGCTGAAGCGGAACATTGGTGTAGTTGTGGTGGGATTTCCTGCT
ACCCCGATCATTGAGTCCAGAGCCAGATTTTGCCTGTCAGCAGCTCATAC
CAAAGAAATACTTGACACTGCTTTGAAGGAGATAGATGAAGTTGGGGATC
TGCTGCAGCTAAAGTACTCTCGCCACCGGCTGGTGCCTCTACTGGACAGG
CCCTTTGATGAGACTACCTATGAAGAGACAGAAGACTGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMRPEPGGCCCRRPMRA
NGCVKNGEVRNGYLRSSTATVAAAGQIHHVTENGGLYKRPFNEAFEETPM
LVAVLTYVGYGVLTLFGYLRDFLRHWRIEKCHHATEREEQKDFVSLYQDF
ENFYTRNLYMRIRDNWNRPICSVPGAKVDIMERKSHDYNWSFKYTGNIIK
GVINMGSYNYLGFARNTGSCQEAAAEVLKEYGAGVCSTRQEIGNLDKHEE
LEKLVARFLGVEAAMTYGMGFATNSMNIPALVGKGCLILSDELNHASLVL
GARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIY
SMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPSGRGVVDYFGLDPE
DVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSMSPPVMEQ
IITSMKCIMGQDGTSLGKECIQQLAENTRYFRRRLKEMGFIIYGNEDSPV
VPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTK
EILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8076
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Estiamted MW of expressed protein : 90120 Daltons