cDNA Clone RFC4

• Plasmid: pEBG6P-RFC4
• Parent Plasmid: pEBG6P
• Expressed: GST-RFC4
• Genbank: NP_002907
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCAAGCATTTCTTAAAGGTACATCCATCAGTACTAAACCCCC
GCTGACCAAGGATCGAGGAGTAGCTGCCAGTGCGGGAAGTAGCGGAGAGA
ACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAATATCGCCCAAAATGT
GTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAAAATC
TTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAA
CTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCT
GAACTTTTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGG
AATACAAGTAGTTCGAGAGAAAGTGAAAAATTTTGCTCAATTAACTGTGT
CAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTTTTAAGATTGTGATT
CTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAAGACG
TACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGTAACT
ATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGC
TTCAAGCCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGC
CAAGAAGGAAAATGTCAAAATTAGTGATGAGGGAATAGCTTATCTTGTTA
AAGTGTCAGAAGGAGACTTAAGAAAAGCCATTACATTTCTTCAAAGCGCT
ACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAAGTGATTACAGACAT
TGCTGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCCTGTC
AGAGTGGCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGAT
GAGGGTCATGCAGCAACTCAGCTCGTCAATCAACTCCATGATGTGGTTGT
AGAAAATAACTTATCTGATAAACAGAAGTCTATTATCACAGAAAAACTTG
CCGAAGTTGACAAATGCCTAGCGGATGGTGCTGATGAACATTTGCAACTC
ATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCTCAGAATTGTTAAGC
GGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMQAFLKGTSISTK
PPLTKDRGVAASAGSSGENKKAKPVPWVEKYRPKCVDEVAFQEEVVAVLK
KSLEGADLPNLLFYGPPGTGKTSTILAAARELFGPELFRLRVLELNASDE
RGIQVVREKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMTSAAQAAL
RRTMEKESKTTRFCLICNYVSRIIEPLTSRCSKFRFKPLSDKIQQQRLLD
IAKKENVKISDEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEITEKVIT
DIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDV
VVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU8033
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for mammalian expression.; Estimated MW of expressed protein : 67056 Daltons