cDNA Clone RFC4

• Plasmid: pGEX6P-1-RFC4
• Parent Plasmid: pGEX6P1
• Expressed: GST-RFC4
• Genbank: NP_002907
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCAAGCATTTCTTAAAGGTACATCCATCAGTACTAAACCCCC
GCTGACCAAGGATCGAGGAGTAGCTGCCAGTGCGGGAAGTAGCGGAGAGA
ACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAATATCGCCCAAAATGT
GTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAAAATC
TTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAA
CTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCT
GAACTTTTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGG
AATACAAGTAGTTCGAGAGAAAGTGAAAAATTTTGCTCAATTAACTGTGT
CAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTTTTAAGATTGTGATT
CTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAAGACG
TACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGTAACT
ATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGC
TTCAAGCCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGC
CAAGAAGGAAAATGTCAAAATTAGTGATGAGGGAATAGCTTATCTTGTTA
AAGTGTCAGAAGGAGACTTAAGAAAAGCCATTACATTTCTTCAAAGCGCT
ACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAAGTGATTACAGACAT
TGCTGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCCTGTC
AGAGTGGCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGAT
GAGGGTCATGCAGCAACTCAGCTCGTCAATCAACTCCATGATGTGGTTGT
AGAAAATAACTTATCTGATAAACAGAAGTCTATTATCACAGAAAAACTTG
CCGAAGTTGACAAATGCCTAGCGGATGGTGCTGATGAACATTTGCAACTC
ATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCTCAGAATTGTTAAGC
GGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMQAFLKGTSISTKPPLTKD
RGVAASAGSSGENKKAKPVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGA
DLPNLLFYGPPGTGKTSTILAAARELFGPELFRLRVLELNASDERGIQVV
REKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMTSAAQAALRRTMEK
ESKTTRFCLICNYVSRIIEPLTSRCSKFRFKPLSDKIQQQRLLDIAKKEN
VKISDEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEITEKVITDIAGVI
PAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDVVVENNL
SDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8028
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.; Estimated MW of expressed protein : 66463 Daltons
• Low Temp: No