cDNA Clone RAS

• Plasmid: pGEX6P-1-RAS
• Parent Plasmid: pGEX6P1
• Expressed: GST-RAS
• Genbank: P01112
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCACGGAATATAAGCTGGTGGTGGTGGGCGCCGGCGGTGTGGGCAA
GAGTGCGCTGACCATCCAGCTGATCCAGAACCATTTTGTGGACGAATACG
ACCCCACTATAGAGGATTCCTACCGGAAGCAGGTGGTCATTGATGGGGAG
ACGTGCCTGTTGGACATCCTGGATACCGCCGGCCAGGAGGAGTACAGCGC
CATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCCTGTGTGTGTTTG
CCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGGAGCAG
ATCAAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAA
CAAGTGTGACCTGGCTGCACGCACTGTGGAATCTCGGCAGGCTCAGGACC
TCGCCCGAAGCTACGGCATCCCCTACATCGAGACCTCGGCCAAGACCCGG
CAGGGAGTGGAGGATGCCTTCTACACGTTGGTGCGTGAGATCCGGCAGCA
CAAGCTGCGGAAGCTGAACCCTCCTGATGAGAGTGGCCCCGGCTGCATGA
GCTGCAAGTGTGTGCTCTCCTGAGAATTCAAAGGCCTACGTCGACGAGCT
CACTAGTCGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTEYKLVVVGAGGVGKSALT
IQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQ
YMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDL
AARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRK
LNPPDESGPGCMSCKCVLS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU8016
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST tag for bacterial expression.; Estimated MW of expressed protein : 47960 Daltons.