cDNA Clone AMPD1

• Plasmid: pGEX6P-1-AMPD1 129-780
• Parent Plasmid: pGEX6P1
• Expressed: GST AMPD1 129-780
• Genbank: NM_000036.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCTGTCCCACATTGATGAATACATTTCCTCATCTCCAACCTACCA
GACCGTGCCTGATTTTCAGAGAGTGCAGATTACTGGTGACTATGCCTCTG
GGGTTACAGTTGAAGATTTTGAAATTGTTTGCAAAGGTCTGTATCGGGCA
CTATGCATACGTGAGAAATACATGCAGAAGTCGTTTCAGAGGTTCCCTAA
AACCCCTTCCAAATACTTGCGGAACATTGATGGTGAGGCTTGGGTAGCAA
ATGAGAGCTTCTATCCAGTCTTTACTCCTCCTGTGAAGAAGGGAGAGGAC
CCCTTCCGAACAGACAACCTTCCTGAAAACCTGGGCTATCACCTCAAAAT
GAAGGACGGTGTAGTTTACGTCTATCCTAATGAAGCAGCAGTCAGCAAAG
ATGAGCCTAAGCCACTTCCTTACCCAAATCTGGACACCTTCTTAGACGAT
ATGAATTTTTTACTTGCTTTAATTGCTCAAGGACCTGTTAAGACCTATAC
CCACCGGCGCCTGAAGTTCCTCTCCTCCAAGTTCCAGGTCCATCAGATGC
TTAACGAGATGGACGAGTTAAAGGAGCTGAAAAACAACCCCCACCGAGAT
TTTTATAACTGCAGGAAGGTGGACACCCATATCCATGCAGCCGCTTGCAT
GAACCAGAAACATCTGCTGCGTTTTATTAAGAAATCTTACCAAATTGATG
CTGACAGAGTGGTCTATAGCACCAAAGAGAAGAATCTGACCCTAAAGGAA
CTTTTTGCTAAATTAAAAATGCATCCTTATGACCTGACTGTTGATTCTCT
GGATGTTCATGCTGGACGCCAGACCTTCCAGCGTTTTGATAAGTTCAATG
ACAAATATAATCCTGTAGGAGCAAGTGAGCTACGGGACCTCTACTTGAAG
ACAGACAATTACATTAATGGGGAATATTTTGCCACTATCATCAAGGAGGT
AGGTGCGGACCTGGTGGAGGCCAAGTACCAGCATGCTGAGCCCCGCCTGT
CCATCTATGGCCGCAGTCCTGATGAGTGGAGCAAACTCTCCTCCTGGTTC
GTCTGCAATCGCATCCACTGCCCCAACATGACATGGATGATCCAGGTTCC
CAGGATCTATGATGTGTTCCGTTCCAAGAATTTCCTTCCACATTTTGGAA
AAATGCTGGAGAATATTTTCATGCCAGTGTTTGAGGCCACCATCAACCCC
CAGGCTGACCCAGAACTCAGTGTCTTCCTCAAGCATATCACTGGCTTTGA
CAGTGTGGATGATGAGTCCAAACACAGTGGCCACATGTTCTCCTCCAAGA
GTCCCAAGCCCCAGGAGTGGACATTGGAAAAGAATCCATCTTACACTTAC
TATGCCTACTACATGTATGCAAACATCATGGTGCTCAACAGCCTGAGAAA
GGAACGAGGCATGAATACGTTTCTGTTCCGACCTCACTGTGGAGAAGCTG
GAGCCCTCACCCATCTCATGACAGCATTCATGATAGCAGATGATATCTCT
CATGGCCTAAATTTAAAAAAGAGTCCCGTGCTACAGTACTTGTTTTTCTT
AGCCCAAATTCCCATCGCCATGTCACCACTAAGTAACAATAGCCTATTTC
TAGAGTATGCCAAAAATCCTTTTTTGGATTTCCTTCAGAAAGGGCTAATG
ATCTCACTGTCTACAGATGACCCAATGCAATTCCACTTTACCAAGGAGCC
CCTAATGGAAGAATATGCTATTGCTGCACAAGTCTTCAAGCTGAGCACCT
GTGATATGTGCGAAGTGGCAAGGAACAGTGTCTTGCAGTGTGGAATTTCT
CATGAGGAGAAAGTAAAGTTTCTGGGCGACAATTACCTTGAGGAAGGCCC
TGCTGGAAATGATATCCGGAGGACAAATGTAGCCCAAATCCGCATGGCCT
ATCGCTATGAAACCTGGTGTTATGAACTCAATTTAATTGCTGAGGGTCTT
AAATCAACAGAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLSHIDEYISSSPTYQTVPD
FQRVQITGDYASGVTVEDFEIVCKGLYRALCIREKYMQKSFQRFPKTPSK
YLRNIDGEAWVANESFYPVFTPPVKKGEDPFRTDNLPENLGYHLKMKDGV
VYVYPNEAAVSKDEPKPLPYPNLDTFLDDMNFLLALIAQGPVKTYTHRRL
KFLSSKFQVHQMLNEMDELKELKNNPHRDFYNCRKVDTHIHAAACMNQKH
LLRFIKKSYQIDADRVVYSTKEKNLTLKELFAKLKMHPYDLTVDSLDVHA
GRQTFQRFDKFNDKYNPVGASELRDLYLKTDNYINGEYFATIIKEVGADL
VEAKYQHAEPRLSIYGRSPDEWSKLSSWFVCNRIHCPNMTWMIQVPRIYD
VFRSKNFLPHFGKMLENIFMPVFEATINPQADPELSVFLKHITGFDSVDD
ESKHSGHMFSSKSPKPQEWTLEKNPSYTYYAYYMYANIMVLNSLRKERGM
NTFLFRPHCGEAGALTHLMTAFMIADDISHGLNLKKSPVLQYLFFLAQIP
IAMSPLSNNSLFLEYAKNPFLDFLQKGLMISLSTDDPMQFHFTKEPLMEE
YAIAAQVFKLSTCDMCEVARNSVLQCGISHEEKVKFLGDNYLEEGPAGND
IRRTNVAQIRMAYRYETWCYELNLIAEGLKSTE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU35774
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for bacterial expression; ; Estimated MW of expressed protein : 102456 Daltons