cDNA Clone CDK18

• Plasmid: pEBG6P-CDK18
• Parent Plasmid: pEBG6P
• Expressed: GST CDK18
• Genbank: AAH11526.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGAACAAGATGAAGAACTTTAAGCGCCGTTTCTCCCTGTC
AGTGCCCCGCACTGAGACCATTGAAGAATCCTTGGCTGAATTCACGGAGC
AATTCAACCAGCTCCACAACCGGCGGAATGAGAACTTGCAGCTCGGTCCT
CTTGGCAGAGACCCCCCGCAGGAGTGCAGCACCTTCTCCCCAACAGACAG
CGGGGAGGAGCCGGGGCAGCTCTCCCCTGGCGTGCAGTTCCAGCGGCGGC
AGAACCAGCGCCGCTTCTCCATGGAGGACGTCAGCAAGAGGCTCTCTCTG
CCCATGGATATCCGCCTGCCCCAGGAATTCCTACAGAAGCTACAGATGGA
GAGCCCAGATCTGCCCAAGCCGCTCAGCCGCATGTCCCGCCGGGCCTCCC
TGTCAGACATTGGCTTTGGGAAACTGGAAACATACGTGAAACTGGACAAA
CTGGGAGAGGGCACCTATGCCACAGTCTTCAAAGGGCGCAGCAAACTGAT
GGAGAACCTTGTGGCCCTGAAAGAGATCCGGCTGGAGCACGAGGAGGGAG
CGCCCTGCACTGCCATCCGAGAGGTGTCTCTGCTGAAGAACCTGAAGCAC
GCCAATATTGTGACCCTGCATGACCTCATCCACACAGATCGGTCCCTCAC
CCTGGTGTTTGAGTACCTGGACAGTGACCTGAAGCAGTATCTGGACCACT
GTGGGAACCTCATGAGCATGCACAACGTCAAGATTTTCATGTTCCAGCTG
CTCCGGGGCCTCGCCTACTGTCACCACCGCAAGATCCTGCACCGGGACCT
GAAGCCCCAGAACCTGCTCATCAACGAGAGGGGGGAGCTGAAGCTGGCCG
ACTTTGGACTGGCCAGGGCCAAGTCAGTGCCCACAAAGACTTACTCCAAT
GAGGTGGTGACCCTGTGGTACAGGCCCCCCGATGTGCTGCTGGGATCCAC
AGAGTACTCCACCCCCATTGATATGTGGGGCGTGGGCTGCATCCACTACG
AGATGGCCACAGGGAGGCCCCTCTTCCCGGGCTCCACAGTCAAGGAGGAG
CTGCACCTCATCTTTCGCCTCCTCGGGACCCCCACAGAAGAGACGTGGCC
CGGCGTGACCGCCTTCTCTGAGTTCCGCACCTACAGCTTCCCCTGCTACC
TCCCGCAGCCGCTCATCAACCACGCGCCCAGGTTGGATACGGATGGCATC
CACCTCCTGAGCAGCCTGCTCCTGTATGAATCCAAGAGTCGCATGTCAGC
AGAGGCTGCCCTGAGTCACTCCTACTTCCGGTCTCTGGGAGAGCGTGTGC
ACCAGCTTGAAGACACTGCCTCCATCTTCTCCCTGAAGGAGATCCAGCTC
CAGAAGGACCCAGGCTACCGAGGCTTGGCCTTCCAGCAGCCAGGACGAGG
GAAGAACAGGCGGCAGAGCATCTTCTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMNKM
KNFKRRFSLSVPRTETIEESLAEFTEQFNQLHNRRNENLQLGPLGRDPPQ
ECSTFSPTDSGEEPGQLSPGVQFQRRQNQRRFSMEDVSKRLSLPMDIRLP
QEFLQKLQMESPDLPKPLSRMSRRASLSDIGFGKLETYVKLDKLGEGTYA
TVFKGRSKLMENLVALKEIRLEHEEGAPCTAIREVSLLKNLKHANIVTLH
DLIHTDRSLTLVFEYLDSDLKQYLDHCGNLMSMHNVKIFMFQLLRGLAYC
HHRKILHRDLKPQNLLINERGELKLADFGLARAKSVPTKTYSNEVVTLWY
RPPDVLLGSTEYSTPIDMWGVGCIHYEMATGRPLFPGSTVKEELHLIFRL
LGTPTEETWPGVTAFSEFRTYSFPCYLPQPLINHAPRLDTDGIHLLSSLL
LYESKSRMSAEAALSHSYFRSLGERVHQLEDTASIFSLKEIQLQKDPGYR
GLAFQQPGRGKNRRQSIF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU35700
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 82414 Daltons