cDNA Clone PEPCK

• Plasmid: pGL4.17 hPEPCK FLAG luciferase
• Parent Plasmid: pET15b 6His 3C
• Expressed: FLAG Luciferase
• Tag: FLAG
• Species: Human

Sequence of Insert

DNA sequence of hPEPCK promoter insert
attcctttctcgaccctcgtctatacccagctgggttgagaccctcagtttcctcatctataaaatgggaaagataacaaaggtacctacatcggagaaaatcatggaatgcatcccatttctggcatgcagagaaagcccagtattatgaagattattatgcccggtgctagagacatagcaagcatttaataaacgtttgagctgaggcttcctagagaatatgagattgcagcaaattggatgcaaaacttctctttacctttttaaatccgtctaattatctcgtatcaggatatagctggtgtggtcacaaagcagggaaaaagcaatcgatatccctgagtaactgcaaaacatggagtggccccaggaaggtgcagggaggaagtcccttttagtttgaaactattttagtgtccgatctttctttctccttctttccccaattttgtatctgggctcaactttttatacatttttcaaaagaagagcagccttggacctaagaaaatctgagcctaaggagctccccaaaagctaggaggtctctatggcatccgtcccagaaacaactaacgcaactcacaggcagcgtgagccacctccatgccccacccactgagcacacactgcaaagtccactcggcgggttaactgggggtcctcagggatcccaccagcagggtggctgtttggctgcttcttcgggtccctgttgaaaccaacagacagtagttagttttgaggcttacttacaaataagctgacagtcagatgaggaagacccatgcttccggccagccccttgaaattgttgtcaatgagtccccactggggcagggagtctctaaggacaggaaaaatgggggcccgagggcatcatctgccaagacctaaggcaactgtacccatacttttgcctgtgtgtcctcaaaacccctctactttatcatgattcttcttccagagaactctgtcctttgaggcaagtctgtaaaccaatgtttgctttttaacattttgacaagggacagttgcgggggaatcattaccgtccttccacgctgtaaatgcaggtaatgaaaggatcggatgaaggtcgttggtgactcactgctctgacgttttacttgaactagctgtttctctcccagaagactcagaacataagcacctggaatggatgggtcgcatttaggactccaaatgagttctgctttccctttttgttttcaatctatgtgctctttcaggtactcaaacctgttaccgttttatcagcaaaatgggactttctaccaatttggtgggatgagtctcgccgggagtgatgtgtgtgtcacggaattgtggtcatgcatcgggatggctaattcactgatgtcagatgctaacgaagtggcctcggctactgagcatcacgtcactgttgggtttagacacaacacgatcttatgtatggatgacagcaccactaataacaaatgccagtgacctaaacggggaataacttcaaatgaaacaaaaagcacctcatatcatatttatcctgaaagacacaactggctgaataaaatctgaatacaggaaacatagcaatgggttgataattttggtggaacttgatgatgtgtgacccacgcccaacacagtgtgacccaagcccccaggagggttctctaagtgagtttggtcggaggctgccattcacagtgacttgtatttgccacagcagctctttctaagcccagtcctgggattatctcagggatcatcccacaccccagcggggagatgggccaagtccccacctgacctcatcttaggtagagaggcttccatggaagtgtgggctggctgtggggggttgagacctagccccgttcttttgacatttcaccctgtgttctccaaactccagcaagcagcttccatagctcattaaatttgactgaaggtctgatggcttgcaaagcaaattctttacaactgttataaaggtttcattgcttaagcatataatctagcaaaactgccaagccccctccactccctaaactctaagacttgccagggaagagaacagtggccagtggactgcaggagtaactgcactcagcccagaactccctggaggacacagggaaggttctggaaaggtggcggtacctcacagggagagacgtggtgctccagggcccaacttgctcatgttcagatcctcactctgcaacccaccagccggctgtgagtggctacacaatctctgcctcagtttccctatctgtacatgggttgttcatagcctccacctcagagggtagtaatgatgccaagctcttagaacagtgctcagcatgtgtgcatcctgtgcctactctatgccaagcactacagagagaaaaatgaacaaaatagggagaaatccctgccctcatggagtttacatcggggcagggggcgggggcggcacagagcagacaatcaatacagtgattagcccccagttaggttaggcatttccaatctttgccaataagccacatatttgcccaagttagggtgcatccttcccatgaactttgactgtgacctttgactatggggtgacatcttatagctgtggtgttttgccaaccagcagctcttggtacacaaaatgtgctgctagcaggtgccccggccaaccttgtccttgacccacctgcctgttaagaaaagggtgttgtgttttgcaacagcagtaaaatgggtcaaggtttagtcagttggaagttgtgtcaaaactcactatggttggttgagggctcgaagtctcccagcattcattaacaactatctgttcaatgattatctccctggggcgtgttgcagtgagttggcccaaagcataactgaccctggccgtgatccagagacctgccccctgacgtcagtggcgagcctccctgggtgcagctgaggggcagggctattcttttccacagta

DNA sequence of promoter insert and FLAG LUC2
ATTCCTTTCTCGACCCTCGTCTATACCCAGCTGGGTTGAGACCCTCAGTT
TCCTCATCTATAAAATGGGAAAGATAACAAAGGTACCTACATCGGAGAAA
ATCATGGAATGCATCCCATTTCTGGCATGCAGAGAAAGCCCAGTATTATG
AAGATTATTATGCCCGGTGCTAGAGACATAGCAAGCATTTAATAAACGTT
TGAGCTGAGGCTTCCTAGAGAATATGAGATTGCAGCAAATTGGATGCAAA
ACTTCTCTTTACCTTTTTAAATCCGTCTAATTATCTCGTATCAGGATATA
GCTGGTGTGGTCACAAAGCAGGGAAAAAGCAATCGATATCCCTGAGTAAC
TGCAAAACATGGAGTGGCCCCAGGAAGGTGCAGGGAGGAAGTCCCTTTTA
GTTTGAAACTATTTTAGTGTCCGATCTTTCTTTCTCCTTCTTTCCCCAAT
TTTGTATCTGGGCTCAACTTTTTATACATTTTTCAAAAGAAGAGCAGCCT
TGGACCTAAGAAAATCTGAGCCTAAGGAGCTCCCCAAAAGCTAGGAGGTC
TCTATGGCATCCGTCCCAGAAACAACTAACGCAACTCACAGGCAGCGTGA
GCCACCTCCATGCCCCACCCACTGAGCACACACTGCAAAGTCCACTCGGC
GGGTTAACTGGGGGTCCTCAGGGATCCCACCAGCAGGGTGGCTGTTTGGC
TGCTTCTTCGGGTCCCTGTTGAAACCAACAGACAGTAGTTAGTTTTGAGG
CTTACTTACAAATAAGCTGACAGTCAGATGAGGAAGACCCATGCTTCCGG
CCAGCCCCTTGAAATTGTTGTCAATGAGTCCCCACTGGGGCAGGGAGTCT
CTAAGGACAGGAAAAATGGGGGCCCGAGGGCATCATCTGCCAAGACCTAA
GGCAACTGTACCCATACTTTTGCCTGTGTGTCCTCAAAACCCCTCTACTT
TATCATGATTCTTCTTCCAGAGAACTCTGTCCTTTGAGGCAAGTCTGTAA
ACCAATGTTTGCTTTTTAACATTTTGACAAGGGACAGTTGCGGGGGAATC
ATTACCGTCCTTCCACGCTGTAAATGCAGGTAATGAAAGGATCGGATGAA
GGTCGTTGGTGACTCACTGCTCTGACGTTTTACTTGAACTAGCTGTTTCT
CTCCCAGAAGACTCAGAACATAAGCACCTGGAATGGATGGGTCGCATTTA
GGACTCCAAATGAGTTCTGCTTTCCCTTTTTGTTTTCAATCTATGTGCTC
TTTCAGGTACTCAAACCTGTTACCGTTTTATCAGCAAAATGGGACTTTCT
ACCAATTTGGTGGGATGAGTCTCGCCGGGAGTGATGTGTGTGTCACGGAA
TTGTGGTCATGCATCGGGATGGCTAATTCACTGATGTCAGATGCTAACGA
AGTGGCCTCGGCTACTGAGCATCACGTCACTGTTGGGTTTAGACACAACA
CGATCTTATGTATGGATGACAGCACCACTAATAACAAATGCCAGTGACCT
AAACGGGGAATAACTTCAAATGAAACAAAAAGCACCTCATATCATATTTA
TCCTGAAAGACACAACTGGCTGAATAAAATCTGAATACAGGAAACATAGC
AATGGGTTGATAATTTTGGTGGAACTTGATGATGTGTGACCCACGCCCAA
CACAGTGTGACCCAAGCCCCCAGGAGGGTTCTCTAAGTGAGTTTGGTCGG
AGGCTGCCATTCACAGTGACTTGTATTTGCCACAGCAGCTCTTTCTAAGC
CCAGTCCTGGGATTATCTCAGGGATCATCCCACACCCCAGCGGGGAGATG
GGCCAAGTCCCCACCTGACCTCATCTTAGGTAGAGAGGCTTCCATGGAAG
TGTGGGCTGGCTGTGGGGGGTTGAGACCTAGCCCCGTTCTTTTGACATTT
CACCCTGTGTTCTCCAAACTCCAGCAAGCAGCTTCCATAGCTCATTAAAT
TTGACTGAAGGTCTGATGGCTTGCAAAGCAAATTCTTTACAACTGTTATA
AAGGTTTCATTGCTTAAGCATATAATCTAGCAAAACTGCCAAGCCCCCTC
CACTCCCTAAACTCTAAGACTTGCCAGGGAAGAGAACAGTGGCCAGTGGA
CTGCAGGAGTAACTGCACTCAGCCCAGAACTCCCTGGAGGACACAGGGAA
GGTTCTGGAAAGGTGGCGGTACCTCACAGGGAGAGACGTGGTGCTCCAGG
GCCCAACTTGCTCATGTTCAGATCCTCACTCTGCAACCCACCAGCCGGCT
GTGAGTGGCTACACAATCTCTGCCTCAGTTTCCCTATCTGTACATGGGTT
GTTCATAGCCTCCACCTCAGAGGGTAGTAATGATGCCAAGCTCTTAGAAC
AGTGCTCAGCATGTGTGCATCCTGTGCCTACTCTATGCCAAGCACTACAG
AGAGAAAAATGAACAAAATAGGGAGAAATCCCTGCCCTCATGGAGTTTAC
ATCGGGGCAGGGGGCGGGGGCGGCACAGAGCAGACAATCAATACAGTGAT
TAGCCCCCAGTTAGGTTAGGCATTTCCAATCTTTGCCAATAAGCCACATA
TTTGCCCAAGTTAGGGTGCATCCTTCCCATGAACTTTGACTGTGACCTTT
GACTATGGGGTGACATCTTATAGCTGTGGTGTTTTGCCAACCAGCAGCTC
TTGGTACACAAAATGTGCTGCTAGCAGGTGCCCCGGCCAACCTTGTCCTT
GACCCACCTGCCTGTTAAGAAAAGGGTGTTGTGTTTTGCAACAGCAGTAA
AATGGGTCAAGGTTTAGTCAGTTGGAAGTTGTGTCAAAACTCACTATGGT
TGGTTGAGGGCTCGAAGTCTCCCAGCATTCATTAACAACTATCTGTTCAA
TGATTATCTCCCTGGGGCGTGTTGCAGTGAGTTGGCCCAAAGCATAACTG
ACCCTGGCCGTGATCCAGAGACCTGCCCCCTGACGTCAGTGGCGAGCCTC
CCTGGGTGCAGCTGAGGGGCAGGGCTATTCTTTTCCACAGTATTTAAAGC
TGGGAGGTTCTGCCACCAAGCACGGCCTTCCCACTGGGAACACAAACTTG
CTGGCGGGAAGAGCCCGGAAAGAAACCTGTGGATCTCCCTTCGAGATAAG
GGCGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGTGGCCTCG
GCGGCCAAGCTTGGCAATCCGGTACTGTTGGTAAGCCACCGCCACCATGG
ACTACAAGGACGACGATGACAAGATGGAAGATGCCAAAAACATTAAGAAG
GGCCCAGCGCCATTCTACCCACTCGAAGACGGGACCGCCGGCGAGCAGCT
GCACAAAGCCATGAAGCGCTACGCCCTGGTGCCCGGCACCATCGCCTTTA
CCGACGCACATATCGAGGTGGACATTACCTACGCCGAGTACTTCGAGATG
AGCGTTCGGCTGGCAGAAGCTATGAAGCGCTATGGGCTGAATACAAACCA
TCGGATCGTGGTGTGCAGCGAGAATAGCTTGCAGTTCTTCATGCCCGTGT
TGGGTGCCCTGTTCATCGGTGTGGCTGTGGCCCCAGCTAACGACATCTAC
AACGAGCGCGAGCTGCTGAACAGCATGGGCATCAGCCAGCCCACCGTCGT
ATTCGTGAGCAAGAAAGGGCTGCAAAAGATCCTCAACGTGCAAAAGAAGC
TACCGATCATACAAAAGATCATCATCATGGATAGCAAGACCGACTACCAG
GGCTTCCAAAGCATGTACACCTTCGTGACTTCCCATTTGCCACCCGGCTT
CAACGAGTACGACTTCGTGCCCGAGAGCTTCGACCGGGACAAAACCATCG
CCCTGATCATGAACAGTAGTGGCAGTACCGGATTGCCCAAGGGCGTAGCC
CTACCGCACCGCACCGCTTGTGTCCGATTCAGTCATGCCCGCGACCCCAT
CTTCGGCAACCAGATCATCCCCGACACCGCTATCCTCAGCGTGGTGCCAT
TTCACCACGGCTTCGGCATGTTCACCACGCTGGGCTACTTGATCTGCGGC
TTTCGGGTCGTGCTCATGTACCGCTTCGAGGAGGAGCTATTCTTGCGCAG
CTTGCAAGACTATAAGATTCAATCTGCCCTGCTGGTGCCCACACTATTTA
GCTTCTTCGCTAAGAGCACTCTCATCGACAAGTACGACCTAAGCAACTTG
CACGAGATCGCCAGCGGCGGGGCGCCGCTCAGCAAGGAGGTAGGTGAGGC
CGTGGCCAAACGCTTCCACCTACCAGGCATCCGCCAGGGCTACGGCCTGA
CAGAAACAACCAGCGCCATTCTGATCACCCCCGAAGGGGACGACAAGCCT
GGCGCAGTAGGCAAGGTGGTGCCCTTCTTCGAGGCTAAGGTGGTGGACTT
GGACACCGGTAAGACACTGGGTGTGAACCAGCGCGGCGAGCTGTGCGTCC
GTGGCCCCATGATCATGAGCGGCTACGTTAACAACCCCGAGGCTACAAAC
GCTCTCATCGACAAGGACGGCTGGCTGCACAGCGGCGACATCGCCTACTG
GGACGAGGACGAGCACTTCTTCATCGTGGACCGGCTGAAGAGCCTGATCA
AATACAAGGGCTACCAGGTAGCCCCAGCCGAACTGGAGAGCATCCTGCTG
CAACACCCCAACATCTTCGACGCCGGGGTCGCCGGCCTGCCCGACGACGA
TGCCGGCGAGCTGCCCGCCGCAGTCGTCGTGCTGGAACACGGTAAAACCA
TGACCGAGAAGGAGATCGTGGACTATGTGGCCAGCCAGGTTACAACCGCC
AAGAAGCTGCGCGGTGGTGTTGTGTTCGTGGACGAGGTGCCTAAAGGACT
GACCGGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCA
AGAAGGGCGGCAAGATCGCCGTGTAA

Amino Acid Sequence

MDYKDDDDKMEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIA
FTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMP
VLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQK
KLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKT
IALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVV
PFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTL
FSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELC
VRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSL
IKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGK
TMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIK
AKKGGKIAV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU35649
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter FLAG tagged LUC2 driven by hPEPCK promoter; ; Estimated MW of expressed protein : 61767 Daltons