cDNA Clone TRAFD1

• Plasmid: pEBG6P-TRAFD1
• Parent Plasmid: pEBG6P
• Expressed: GST TRAFD1
• Genbank: NM_006700.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCTGAATTTCTAGATGACCAGGAAACTCGACTGTGTGACAA
CTGCAAAAAAGAAATTCCTGTGTTTAACTTTACCATCCATGAGATCCACT
GTCAAAGGAACATTGGTATGTGTCCTACCTGTAAGGAACCATTTCCCAAA
TCTGACATGGAGACTCACATGGCTGCAGAACACTGTCAGGTGACCTGCAA
ATGTAACAAGAAGTTGGAGAAGAGGCTGTTAAAGAAGCATGAGGAGACTG
AGTGCCCTTTGCGGCTTGCTGTCTGCCAGCACTGTGATTTAGAACTTTCC
ATTCTCAAACTGAAGGAACATGAAGATTATTGTGGTGCCCGGACGGAACT
ATGTGGCAACTGTGGTCGCAATGTCCTTGTGAAAGATCTGAAGACTCACC
CTGAAGTTTGTGGGAGAGAGGGGGAGGAAAAGAGAAATGAGGTTGCCATA
CCTCCTAATGCATATGATGAATCTTGGGGTCAGGATGGAATCTGGATTGC
ATCCCAACTCCTCAGACAAATTGAGGCTCTGGACCCACCCATGAGGCTGC
CGCGAAGGCCCCTGAGAGCCTTTGAATCAGATGTTTTCCACAATAGAACT
ACCAACCAAAGGAACATTACAGCCCAGGTTTCAATTCAGAATAATCTGTT
TGAAGAACAAGAGAGGCAGGAAAGGAATAGAGGCCAACAGCCCCCCAAAG
AGGGTGGTGAAGAGAGTGCAAACTTGGACTTCATGTTGGCCCTAAGTCTG
CAAAATGAAGGCCAAGCCTCCAGTGTGGCAGAGCAGGACTTCTGGAGGGC
CGTATGTGAGGCCGACCAGTCTCATGGCGGTCCCAGGTCTCTCAGTGACA
TAAAGGGTGCAGCTGACGAGATCATGTTGCCTTGTGAATTTTGTGAGGAG
CTCTACCCAGAGGAACTGCTGATTGACCATCAGACAAGCTGTAACCCTTC
ACGTGCCTTACCTTCACTCAATACTGGCAGCTCTTCCCCCAGAGGGGTGG
AGGAACCTGATGTCATCTTCCAGAACTTCTTGCAACAGGCTGCAAGTAAC
CAGTTAGACTCTTTGATGGGCCTGAGCAATTCACACCCTGTGGAGGAGAG
CATCATTATCCCATGTGAATTCTGTGGGGTACAGCTGGAAGAGGAGGTGC
TGTTCCATCACCAGGACCAGTGTGACCAACGCCCAGCCACTGCAACCAAC
CATGTGACAGAGGGGATTCCTAGACTGGATTCCCAGCCTCAAGAGACCTC
ACCAGAGCTGCCCAGGAGGCGTGTCAGACACCAGGGAGACCTGTCTTCTG
GTTACCTGGATGATACTAAGCAGGAAACAGCTAATGGGCCCACCTCCTGT
CTGCCTCCCAGCCGACCCATTAACAATATGACAGCTACCTATAACCAGCT
ATCGAGATCAACATCAGGCCCCAGACCTGGGTGCCAGCCCAGCTCTCCTT
GTGTGCCGAAGCTCAGCAACTCAGACAGCCAGGACATCCAGGGGCGGAAT
CGAGACAGCCAGAATGGGGCCATAGCCCCTGGGCACGTTTCAGTGATTCG
CCCTCCTCAAAATCTCTACCCAGAAAACATTGTGCCCTCTTTCTCCCCTG
GGCCTTCAGGGAGATACGGAGCTAGTGGTAGGAGTGAAGGTGGCAGGAAT
TCCCGGGTCACCCCTGCAGCTGCCAACTACCGCAGCAGAACTGCAAAGGC
AAAGCCTTCCAAGCAACAGGGAGCTGGGGATGCAGAAGAGGAAGAGGAGG
AGTAAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAEFLDDQETRLC
DNCKKEIPVFNFTIHEIHCQRNIGMCPTCKEPFPKSDMETHMAAEHCQVT
CKCNKKLEKRLLKKHEETECPLRLAVCQHCDLELSILKLKEHEDYCGART
ELCGNCGRNVLVKDLKTHPEVCGREGEEKRNEVAIPPNAYDESWGQDGIW
IASQLLRQIEALDPPMRLPRRPLRAFESDVFHNRTTNQRNITAQVSIQNN
LFEEQERQERNRGQQPPKEGGEESANLDFMLALSLQNEGQASSVAEQDFW
RAVCEADQSHGGPRSLSDIKGAADEIMLPCEFCEELYPEELLIDHQTSCN
PSRALPSLNTGSSSPRGVEEPDVIFQNFLQQAASNQLDSLMGLSNSHPVE
ESIIIPCEFCGVQLEEEVLFHHQDQCDQRPATATNHVTEGIPRLDSQPQE
TSPELPRRRVRHQGDLSSGYLDDTKQETANGPTSCLPPSRPINNMTATYN
QLSRSTSGPRPGCQPSSPCVPKLSNSDSQDIQGRNRDSQNGAIAPGHVSV
IRPPQNLYPENIVPSFSPGPSGRYGASGRSEGGRNSRVTPAAANYRSRTA
KAKPSKQQGAGDAEEEEEE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU35545
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N ter GST for mammalian expression; ; Estimated MW of expressed protein : 64983 Daltons