cDNA Clone STIM1 (mouse)

• Plasmid: pGEM-T-STIM1-GFP
• Parent Plasmid: pGEM-T Easy
• Expressed: STIM1-GFP
• Genbank: NM_009287
• Tag: GFP
• Species: Mouse

Sequence of Insert

GGATCCGCCACCATGGATGTGTGCGCCCGTCTTGCCCTGTGGCTTCTTTGGGGGCTCCTTCTGCATCAGGGCCAGAGTCTCAGCCATAGTCACAGTGAAAAGAATACAGGAGCTAGCTCCGGGGCGACTTCTGAAGAGTCTACCGAAGCAGAGTTTTGCCGAATTGACAAGCCCCTGTGCCACAGTGAGGATGAGAAGCTCAGCTTTGAGGCCGTCCGAAACATCCATAAGCTGATGGATGACGATGCCAATGGTGATGTGGATGTGGAAGAAAGTGATGAGTTCCTAAGGGAAGACCTCAATTACCATGACCCAACAGTGAAACATAGCACCTTCCATGGTGAGGATAAGCTTATCAGCGTGGAGGACCTGTGGAAGGCGTGGAAATCATCAGAAGTGTACAACTGGACTGTGGACGAGGTGATACAGTGGCTCATTACGTATGTGGAGCTGCCACAGTATGAGGAGACCTTCCGGAAGTTGCAGCTTACTGGCCACGCCATGCCAAGGCTAGCAGTAACCAACACCACCATGACAGGGACTGTACTGAAGATGACAGATCGGAGCCACAGGCAGAAGCTGCAGCTGAAGGCCCTGGACACAGTGCTGTTTGGGCCTCCTCTCTTGACTCGGCATAATCACCTGAAGGACTTCATGCTGGTGGTGTCTATCGTTATTGGTGTGGGTGGCTGCTGGTTTGCCTATATCCAGAACCGTTACTCTAAGGAGCACATGAAGAAAATGATGAAGGATCTGGAAGGGTTACACCGGGCTGAGCAGAGTCTGCATGACCTTCAGGAAAGGCTGCACAAGGCCCAGGAGGAGCACCGAACTGTGGAAGTAGAGAAAGTCCACCTGGAGAAGAAGCTGCGAGATGAGATCAACCTTGCCAAGCAGGAAGCTCAGCGGCTGAAGGAGCTGAGGGAGGGTACTGAGAATGAGAGGAGCCGTCAAAAATATGCTGAGGAAGAGCTGGAGCAGGTTCGGGAGGCCTTGAGGAAAGCAGAGAAGGAGCTGGAATCACACAGCTCATGGTATGCTCCTGAGGCCCTGCAGAAGTGGCTGCAGCTGACCCATGAGGTGGAGGTGCAGTACTACAACATCAAGAAGCAAAATGCAGAGAGGCAGCTGCTGGTGGCCAAGGAGGGGGCTGAGAAAATAAAAAAGAAGAGAAACACGCTTTTTGGTACCTTCCATGTGGCCCACAGCTCTTCCCTGGATGATGTGGATCATAAAATCCTAACTGCTAAGCAAGCTCTGAGTGAGGTGACAGCGGCACTGAGGGAGCGCCTGCACCGGTGGCAGCAGATCGAGATCCTCTGCGGTTTCCAGATTGTCAATAACCCCGGCATCCACTCCTTGGTGGCTGCTCTCAACATCGACCCCAGCTGGATGGGCAGCACCCGCCCTAACCCCGCCCACTTCATCATGACTGACGATGTGGATGACATGGATGAGGAGATTGTGTCGCCCTTGTCCATGCAGTCCCCCAGCCTGCAGAGCAGTGTCCGGCAGCGCCTGACGGAGCCACAGCTTGGCCTGGGATCTCAGAGGGATTTGACCCATTCCGATTCGGAGTCCTCCCTCCACATGAGTGACCGCCAGCGTGTGGCCCCCAAGCCTCCTCAGATGGGCCGTGCTGCAGATGAAGCTCTCAATGCCATGCCTTCCAATGGCAGCCATCGGCTGATTGAGGGGGTCCATCCAGGATCTCTGGTGGAGAAACTGCCTGACAGCCCTGCTCTGGCCAAGAAGACATTTATGGCGTTGAACCATGGCCTAGACAAGGCCCACAGCCTGATGGAGCTGAACCCCTCAGTCCCACCTGGTGGCTCCCCACTTTTGGATTCTTCCCATTCTCTTAGCCCCAGTTCCCCAGACCCAGACACACCATCTCCAGTTGGGGACAACCGAGCTCTGCAGGGTAGCCGAAACACACGAATTCCCCACTTGGCTGGCAAGAAGGCAATGGCTGAGGAGGATAATGGTTCCATTGGTGAGGAGACAGACTCCAGTCCAGGCAGGAAGAAGTTTCCTCTCAAAATTTTTAAGAAGCCTCTTAAGAAGGCGGCCGCGATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGCTCGAG

Amino Acid Sequence

MDVCARLALWLLWGLLLHQGQSLSHSHSEKNTGASSGATSEESTEAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQLGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMGRAADEALNAMPSNGSHRLIEGVHPGSLVEKLPDSPALAKKTFMALNHGLDKAHSLMELNPSVPPGGSPLLDSSHSLSPSSPDPDTPSPVGDNRALQGSRNTRIPHLAGKKAMAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKKAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU17467
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: parental pGEM-T Easy (modified) Can linearise with Nde1 after Sal1 site