cDNA Clone TRIM27

• Synonyms: RFP; RNF76
• Plasmid: pCMV-HA TRIM27 C53/A+C56/A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-TRIM27 C53/A+C56/A
• Genbank: NM_006510.4 GI:115387097 with change g1336a (residue change E446K) (EST-derived)
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCGGCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAGACTAACGTGTCGGCCCCGCAGGCCCGGGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGGCACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGAGAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTGCGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGCTCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGACCATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGAACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGATTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGAGTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCATCAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCCTGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTGCAGGACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGAACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCCAAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATGCAGTCAGATATGGAGAAAATCCAAGAATTAAGAGAGGCTCAGTTATACTCAGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTGATCCTCTCTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCTGACAACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGCTTCATCGCCGGGAGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGGAGTAACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAAAGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTGCGGACCCCGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTAAGGTCTCCTTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTACCTTTTGTGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGAAAAGTGCAGCTCCTCTGATCATCTGCCCCATGAGTGGGATAGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCTTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCA
CLARCWGTAETNVSAPQARETFPQRHMRPNRHLANVTQLVKQLRTERPSG
PGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEG
FKEQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQ
LYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEE
KQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLT
ESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQV
RYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVC
EDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGI
FLDYDAGKVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLI
ICPMSGIDGFSGHVGNHGHSMETSP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU17179
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee