cDNA Clone WNK2

• Plasmid: pGEX6P1 WNK2 M1-D627 Kdead D342A
• Parent Plasmid: pGEX6P1
• Expressed: GST-WNK2 M1-D627 Kdead D342A
• Genbank: NM_006648.3 GI:32455272 with silent changes c267t and t1011c
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGGACGGCGATGGCGGCCGCCGAGACGTCCCCGGCACGCTGATGGAGCCCGGGCGCGGCGCGGGGCCCGCGGGCATGGCGGAGCCTCGGGCGAAGGCGGCGCGGCCGGGGCCCCAGCGCTTTCTGCGGCGCAGCGTGGTAGAGTCGGACCAGGAGGAGCCGCCGGGCTTGGAGGCAGCCGAGGCGCCGGGCCCGCAGCCCCCGCAGCCCCTGCAGCGCCGGGTGCTTCTGCTCTGCAAGACGCGCCGCCTCATCGCGGAGCGCGCTCGCGGACGCCCCGCCGCCCCCGCGCCCGCAGCGCTGGTAGCGCAGCCGGGAGCCCCCGGAGCCCCCGCGGACGCCGGCCCCGAGCCCGTGGGCACGCAGGAGCCCGGCCCGGACCCCATCGCAGCCGCTGTCGAAACCGCGCCTGCCCCCGACGGCGGCCCCAGGGAGGAGGCGGCGGCGACCGTGAGGAAGGAGGATGAGGGGGCGGCCGAGGCGAAGCCTGAGCCCGGGCGCACTCGCCGGGACGAGCCCGAAGAGGAGGAGGACGACGAGGACGACCTCAAGGCCGTGGCCACCTCTCTGGACGGCCGCTTCCTCAAGTTCGACATCGAGCTGGGCCGCGGTTCCTTCAAGACGGTCTACAAGGGGCTGGACACGGAGACCTGGGTGGAGGTGGCCTGGTGTGAGCTGCAGGACCGGAAGCTCACCAAGCTGGAGCGGCAGCGGTTCAAGGAAGAGGCTGAGATGCTGAAAGGCCTGCAGCACCCCAACATCGTGCGCTTCTACGACTTCTGGGAGTCCAGCGCCAAGGGCAAGCGGTGCATTGTGCTGGTGACGGAGCTGATGACCTCAGGGACGCTGAAGACATACCTGAAGCGGTTCAAGGTGATGAAGCCCAAGGTTCTCCGCAGCTGGTGCCGGCAGATCCTGAAGGGCCTGCTGTTCCTGCACACAAGGACGCCACCCATCATCCACCGAGACCTGAAATGTGACAATATTTTCATCACCGGACCAACTGGGTCCGTGAAGATTGGCGCCTTGGGCCTGGCCACTCTGAAAAGAGCGTCATTTGCCAAAAGTGTGATAGGTACTCCCGAGTTCATGGCGCCCGAGATGTACGAGGAGCACTACGATGAGTCCGTGGACGTCTATGCCTTTGGGATGTGCATGCTGGAGATGGCCACCTCGGAGTACCCCTACTCGGAGTGCCAGAATGCGGCCCAGATCTACCGCAAGGTCACCTGTGGTATCAAGCCGGCCAGCTTTGAGAAAGTGCACGATCCTGAAATCAAGGAGATTATTGGGGAGTGTATCTGCAAAAACAAGGAGGAAAGGTACGAGATCAAAGACCTGCTGAGCCACGCCTTCTTCGCAGAGGACACAGGCGTGAGGGTGGAGCTCGCGGAGGAGGACCACGGCAGGAAGTCCACCATCGCCCTGAGGCTCTGGGTGGAAGACCCCAAGAAACTGAAGGGAAAGCCCAAGGACAATGGAGCCATAGAGTTCACCTTCGACCTGGAGAAGGAGACGCCGGATGAGGTGGCCCAAGAGATGATTGAGTCTGGATTCTTCCACGAGAGTGACGTCAAGATCGTGGCCAAGTCCATCCGTGACCGCGTGGCCTTGATCCAGTGGCGGCGGGAGAGGATCTGGCCCGCGCTGCAGCCCAAGGAGCAGCAGGATGTGGGCAGCCCGGACAAGGCCAGGGGTCCGCCGGTGCCCCTGCAGGTCCAGGTGACCTACCATGCACAGGCTGGGCAGCCCGGGCCACCAGAGCCCGAGGAGCCGGAGGCCGACCAGCACCTCCTGCCACCTACGTTGCCGACCAGCGCCACCTCCCTGGCCTCGGACAGCACCTTCGACAGCGGCCAGGGCTCTACCGTGTACTCAGACTGAATCGATGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMDGDGGRRDVPGTLME
PGRGAGPAGMAEPRAKAARPGPQRFLRRSVVESDQEEPPGLEAAEAPGPQ
PPQPLQRRVLLLCKTRRLIAERARGRPAAPAPAALVAQPGAPGAPADAGP
EPVGTQEPGPDPIAAAVETAPAPDGGPREEAAATVRKEDEGAAEAKPEPG
RTRRDEPEEEEDDEDDLKAVATSLDGRFLKFDIELGRGSFKTVYKGLDTE
TWVEVAWCELQDRKLTKLERQRFKEEAEMLKGLQHPNIVRFYDFWESSAK
GKRCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLLFLHTRT
PPIIHRDLKCDNIFITGPTGSVKIGALGLATLKRASFAKSVIGTPEFMAP
EMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTCGIKPA
SFEKVHDPEIKEIIGECICKNKEERYEIKDLLSHAFFAEDTGVRVELAEE
DHGRKSTIALRLWVEDPKKLKGKPKDNGAIEFTFDLEKETPDEVAQEMIE
SGFFHESDVKIVAKSIRDRVALIQWRRERIWPALQPKEQQDVGSPDKARG
PPVPLQVQVTYHAQAGQPGPPEPEEPEADQHLLPPTLPTSATSLASDSTF
DSGQGSTVYSD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU13805
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.