cDNA Clone WNK3 (mouse)

• Plasmid: pGEX-6-WNK3 (mouse) 1145-1508
• Parent Plasmid: pGEX6P1
• Expressed: GST-WNK3 (mouse) 1145-1508
• Genbank: XP_914679
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCTCTCCATTTTCTTCCTCTGCTGAAGATATACTATCCTATTCAATGCCAGAAGTCATAGCCATCAGTCACTGTGGGATTCAAGATAGCCCTGCACAATCCCCAAATTTCCAACAAACAGGCTCTAAGATTCTGTCCAATGTGGCTGCAAGTCAGCCTGCTCATATATCAGTGTTCAAAAAAGACCTGAATGTGATAACTTCTGTACCCAGCGAGTTATGTTTACATGAGATGTCCCCAGATGCTTCACTTCCAGGCGATCCAGAGGCCTATCCTGCTGCTGTGTCAAGCGATGGAACCATTCATCTGCAGACAGGAGTGGAAACAGAAGATAAAAGATCAGCAATTGCTTCTGATCCCATTCCTCTGACACGGGAGTTCTCATCTGATACTAGGGCTTTGAGTAGATGTAAAGCGATGAGTGGATCATTTCAGCGGGGTCGGTTCCAGGTGATTACTGTTCCTCAGCAGCAGCCAGTAAAAATGATGTCTTTTGGGAAAGATCACAGACCGCCATTCAACAAAACAACAGTCCAGTCTAGTGAGCAAGCACTAACCTTTGCTGAAGCTGCAGTGTCGCAGTTGATCGAAGTGGAGCCTGCCATGCCCACTCATAAAGCTTCAGTTTCTTCACGCAAGTTGCGAACTCTTTATGAAACCTTTAAAGAAGATAAAGGAGATCCTGAACAAGGTGACATCGTATCTTACAGCACAGCTTGTGAGACCAGTGTCTCTTCAGTCGCTACAGAAAAGAATGTAACTTCAACCACAGAAGTTAGTGTGCAGTCTGGGTCTGAACCGTTAGATAAAGAAAAAAATGAGTCAACTCCTGGGAAACAGACATGTACAAATGAATTTTCAGCCACTCTTGCTGGTAATAGAAAGTCCGTGACAAAAACTCGTCCAGAGGGTGATCAGTATTTACCACTCCGTGAAGAGCAAGCCTATGCTCAATAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSPFSSSAEDILSYSMPEVIAISHCGIQDSPAQSPNFQQTGSKILSNVAASQPAHISVFKKDLNVITSVPSELCLHEMSPDASLPGDPEAYPAAVSSDGTIHLQTGVETEDKRSAIASDPIPLTREFSSDTRALSRCKAMSGSFQRGRFQVITVPQQQPVKMMSFGKDHRPPFNKTTVQSSEQALTFAEAAVSQLIEVEPAMPTHKASVSSRKLRTLYETFKEDKGDPEQGDIVSYSTACETSVSSVATEKNVTSTTEVSVQSGSEPLDKEKNESTPGKQTCTNEFSATLAGNRKSVTKTRPEGDQYLPLREEQAYAQ*RP

• Vector Type: Bacterial
• DU Number: DU13575
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: (our clone is not identical with this sequence, however our is better match to human wnk3 then XP_914679 – therefore I think, our is the major splice variant); Amplified from mouse brain RNA.; ; All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.
• Low Temp: No