cDNA Clone NUAK1

• Plasmid: Lentiviral-HA NUAK1 wt
• Parent Plasmid: pHR SIN CSGW
• Expressed: HA-NUAK1 wt
• Genbank: NM_014840
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCACTAGTGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAAGGGGCCGCCGCGCCTGTGGCGGGGGACCGCCCCGACTTGGGGCTGGGGGCGCCGGGCTCTCCCCGAGAGGCGGTGGCGGGGGCGACTGCAGCCCTGGAGCCCAGGAAGCCGCACGGGGTGAAGCGGCATCACCACAAGCACAACTTGAAGCACCGCTACGAGCTGCAGGAGACCCTGGGCAAAGGCACCTACGGCAAAGTCAAGCGGGCCACCGAGAGGTTTTCTGGCCGAGTGGTTGCTATAAAATCCATTCGTAAGGACAAAATTAAGGATGAACAAGACATGGTTCACATCAGACGAGAGATTGAGATCATGTCATCTCTCAACCATCCTCATATCATCAGTATTTATGAAGTGTTTGAGAACAAAGATAAGATTGTGATCATCATGGAATATGCCAGCAAAGGGGAGCTGTACGATTACATCAGTGAGCGGCGACGCCTCAGTGAGAGGGAGACCCGGCACTTCTTCCGGCAGATCGTCTCTGCTGTGCACTATTGTCACAAGAACGGTGTGGTCCACCGGGACTTGAAGCTGGAAAATATACTGCTCGATGACAACTGCAATATTAAGATTGCTGACTTTGGGCTTTCCAACCTGTACCAGAAGGATAAGTTCTTACAAACGTTTTGTGGGAGTCCACTCTATGCATCTCCTGAGATTGTCAATGGGAGACCTTACCGAGGGCCAGAGGTGGACAGCTGGGCCCTGGGTGTGTTGCTTTACACTCTTGTTTATGGAACAATGCCCTTCGATGGTTTCGATCACAAAAACCTCATTCGGCAAATCAGCAGCGGAGAGTACCGGGAGCCAACACAGCCCTCAGATGCTCGAGGACTCATACGGTGGATGCTGATGGTGAACCCCGATCGCCGGGCCACTATTGAGGACATTGCCAACCACTGGTGGGTGAACTGGGGCTATAAGAGCAGCGTGTGTGACTGTGATGCCCTCCATGACTCTGAGTCCCCACTCCTGGCTCGGATCATTGACTGGCACCACCGTTCCACAGGGCTGCAGGCTGACACCGAAGCCAAAATGAAGGGCCTGGCCAAACCCACGACCTCTGAGGTCATGCTAGAGCGGCAGCGGTCGCTGAAGAAATCCAAGAAAGAGAATGACTTTGCTCAGTCTGGTCAGGATGCAGTGCCTGAAAGCCCATCCAAGTTGAGTTCTAAGAGGCCCAAGGGGATCCTGAAGAAGCGAAGCAACAGCGAGCATCGCTCTCACAGCACTGGCTTCATTGAAGGTGTAGTTGGTCCTGCCTTACCCTCTACTTTCAAGATGGAGCAGGACTTGTGCAGGACTGGCGTGCTCCTCCCAAGCTCACCAGAGGCAGAGGTGCCGGGAAAACTCAGCCCCAAGCAGTCGGCCACGATGCCCAAGAAAGGCATCTTGAAAAAGACCCAGCAGAGAGAATCAGGTTACTACTCTTCCCCAGAGCGCAGTGAGTCTTCGGAGCTGTTGGACAGTAATGATGTGATGGGCAGCAGCATCCCCTCCCCCAGCCCCCCGGACCCAGCCAGGGTAACCTCCCACAGCCTCTCCTGCCGGAGGAAGGGCATCTTGAAACACAGCAGCAAATACTCAGCGGGCACCATGGACCCAGCCCTGGTCAGCCCTGAAATGCCCACACTGGAATCCCTGTCAGAGCCTGGTGTCCCTGCCGAGGGCCTCTCCCGGAGCTACAGCCGCCCTTCCAGTGTCATCAGCGATGACAGCGTGCTGTCCAGCGACTCTTTTGACTTGCTGGATTTGCAGGAGAATCGCCCTGCCCGCCAGCGCATCCGCAGCTGCGTCTCTGCAGAAAACTTCCTCCAGATCCAGGACTTTGAGGGGCTCCAGAACCGGCCCCGGCCCCAGTACCTGAAGCGGTACCGGAACCGGCTGGCAGACAGCAGCTTCTCCCTCCTCACAGACATGGATGATGTGACTCAGGTCTACAAGCAAGCGCTGGAGATCTGCAGCAAGCTCAACTAGAAGGGCGAATTCCCGGGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAEGAAAPVAGDRPDLGLGAPGSPREAVAGATAALEPRKPHGVKRHHHKHNLKHRYELQETLGKGTYGKVKRATERFSGRVVAIKSIRKDKIKDEQDMVHIRREIEIMSSLNHPHIISIYEVFENKDKIVIIMEYASKGELYDYISERRRLSERETRHFFRQIVSAVHYCHKNGVVHRDLKLENILLDDNCNIKIADFGLSNLYQKDKFLQTFCGSPLYASPEIVNGRPYRGPEVDSWALGVLLYTLVYGTMPFDGFDHKNLIRQISSGEYREPTQPSDARGLIRWMLMVNPDRRATIEDIANHWWVNWGYKSSVCDCDALHDSESPLLARIIDWHHRSTGLQADTEAKMKGLAKPTTSEVMLERQRSLKKSKKENDFAQSGQDAVPESPSKLSSKRPKGILKKRSNSEHRSHSTGFIEGVVGPALPSTFKMEQDLCRTGVLLPSSPEAEVPGKLSPKQSATMPKKGILKKTQQRESGYYSSPERSESSELLDSNDVMGSSIPSPSPPDPARVTSHSLSCRRKGILKHSSKYSAGTMDPALVSPEMPTLESLSEPGVPAEGLSRSYSRPSSVISDDSVLSSDSFDLLDLQENRPARQRIRSCVSAENFLQIQDFEGLQNRPRPQYLKRYRNRLADSSFSLLTDMDDVTQVYKQALEICSKLN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU13246
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: no puromycin resistance in this lentiviral plasmid