cDNA Clone LKB1; STRAD; MO25

• Plasmid: pMultiBac STRAD, MO25, His-LKB1
• Parent Plasmid: pFBDM
• Genbank: -
• Tag: 6His
• Species: Human

Sequence of Insert

LKB1
CGGAATTCTATAAATATGGCACACCATCACCATCACCATGAAAACCTGTATTTTCAGGGCATGGAGGTGGTGGACCCGCAGCAGCTGGGCATGTTCACGGAGGGCGAGCTGATGTCGGTGGGTATGGACACGTTCATCCACCGCATCGACTCCACCGAGGTCATCTACCAGCCGCGCCGCAAGCGGGCCAAGCTCATCGGCAAGTACCTGATGGGGGACCTGCTGGGGGAAGGCTCTTACGGCAAGGTGAAGGAGGTGCTGGACTCGGAGACGCTGTGCAGGAGGGCCGTCAAGATCCTCAAGAAGAAGAAGTTGCGAAGGATCCCCAACGGGGAGGCCAACGTGAAGAAGGAAATTCAACTACTGAGGAGGTTACGGCACAAAAATGTCATCCAGCTGGTGGATGTGTTATACAACGAAGAGAAGCAGAAAATGTATATGGTGATGGAGTACTGCGTGTGTGGCATGCAGGAAATGCTGGACAGCGTGCCGGAGAAGCGTTTCCCAGTGTGCCAGGCCCACGGGTACTTCTGTCAGCTGATTGACGGCCTGGAGTACCTGCATAGCCAGGGCATTGTGCACAAGGACATCAAGCCGGGGAACCTGCTGCTCACCACCGGTGGCACCCTCAAAATCTCCGACCTGGGCGTGGCCGAGGCACTGCACCCGTTCGCGGCGGACGACACCTGCCGGACCAGCCAGGGCTCCCCGGCTTTCCAGCCGCCCGAGATTGCCAACGGCCTGGACACCTTCTCCGGCTTCAAGGTGGACATCTGGTCGGCTGGGGTCACCCTCTACAACATCACCACGGGTCTGTACCCCTTCGAAGGGGACAACATCTACAAGTTGTTTGAGAACATCGGGAAGGGGAGCTACGCCATCCCGGGCGACTGTGGCCCCCCGCTCTCTGACCTGCTGAAAGGGATGCTTGAGTACGAACCGGCCAAGAGGTTCTCCATCCGGCAGATCCGGCAGCACAGCTGGTTCCGGAAGAAACATCCTCCGGCTGAAGCACCAGTGCCCATCCCACCGAGCCCAGACACCAAGGACCGGTGGCGCAGCATGACTGTGGTGCCGTACTTGGAGGACCTGCACGGCGCGGACGAGGACGAGGACCTCTTCGACATCGAGGATGACATCATCTACACTCAGGACTTCACGGTGCCCGGACAGGTCCCAGAAGAGGAGGCCAGTCACAATGGACAGCGCCGGGGCCTCCCCAAGGCCGTGTGTATGAACGGCACAGAGGCGGCGCAGCTGAGCACCAAATCCAGGGCGGAGGGCCGGGCCCCCAACCCTGCCCGCAAGGCCTGCTCCGCCAGCAGCAAGATCCGCCGGCTGTCGGCCTGCAAGCAGCAGTGAAAGGGCGAATTC
STRAD:
GAATTCTATAAATATGTCATTTCTTGTAAGTAAACCAGAGCGAATCAGGCGGTGGGTCTCGGAAAAGTTCATTGTTGAGGGCTTAAGAGATTTGGAACTATTTGGAGAGCAGCCTCCGGGTGACACTCGGAGAAAAACCAATGATGCGAGCTCAGAGTCAATAGCATCCTTCTCTAAACAGGAGGTCATGAGTAGCTTTCTGCCAGAGGGAGGGTGTTACGAGCTGCTCACTGTGATAGGCAAAGGATTTGAGGACCTGATGACTGTGAATCTAGCAAGGTACAAACCAACAGGAGAGTACGTGACTGTACGGAGGATTAACCTAGAAGCTTGTTCCAATGAGATGGTAACATTCTTGCAGGGCGAGCTGCATGTCTCCAAACTCTTCAACCATCCCAATATCGTGCCATATCGAGCCACTTTTATTGCAGACAATGAGCTGTGGGTTGTCACATCATTCATGGCATACGGTTCTGCAAAAGATCTCATCTGTACACACTTCATGGATGGCATGAATGAGCTGGCGATTGCTTACATCCTGCAGGGGGTGCTGAAGGCCCTCGACTACATCCACCACATGGGATATGTACACAGGAGTGTCAAAGCCAGCCACATCCTGATCTCTGTGGATGGGAAGGTCTACCTGTCTGGTTTGCGCAGCAACCTCAGCATGATAAGCCATGGGCAGCGGCAGCGAGTGGTCCACGATTTTCCCAAGTACAGTGTCAAGGTTCTGCCGTGGCTCAGCCCCGAGGTCCTCCAGCAGAATCTCCAGGGTTATGATGCCAAGTCTGACATCTACAGTGTGGGAATCACAGCCTGTGAACTGGCCAACGGCCATGTCCCCTTTAAGGATATGCCTGCCACCCAGATGCTGCTAGAGAAACTGAACGGCACAGTGCCCTGCCTGTTGGATACCAGCACCATCCCCGCTGAGGAGCTGACCATGAGCCCTTCGCGCTCAGTGGCCAACTCTGGCCTGAGTGACAGCCTGACCACCAGCACCCCCCGGCCCTCCAACGGTGACTCGCCCTCCCACCCCTACCACCGAACCTTCTCCCCCCACTTCCACCACTTTGTGGAGCAGTGCCTTCAGCGCAACCCGGATGCCAGGCCCAGTGCCAGCACCCTCCTGAACCACTCTTTCTTCAAGCAGATCAAGCGACGTGCCTCAGAGGCTTTGCCCGAATTGCTTCGTCCTGTCACCCCCATCACCAATTTTGAGGGCAGCCAGTCTCAGGACCACAGTGGAATCTTTGGCCTGGTAACAAACCTGGAAGAGCTGGAGGTGGACGATTGGGAGTTCTGAGCGGCCGC
MO25
CTCGAGTATAAATATGCCGTTCCCGTTTGGGAAGTCTCACAAATCTCCAGCAGACATTGTGAAGAATCTGAAGGAGAGCATGGCTGTTCTGGAAAAGCAAGACATTTCTGATAAAAAAGCAGAAAAGGCTACAGAAGAAGTTTCCAAAAATCTGGTTGCCATGAAAGAAATTCTGTATGGCACAAATGAAAAAGAGCCTCAGACAGAAGCAGTAGCTCAACTTGCTCAAGAACTCTATAATAGTGGGCTCCTTAGCACCCTGGTAGCTGATTTACAGCTCATTGACTTTGAGGGCAAAAAAGACGTGGCTCAAATTTTCAACAATATTCTCAGAAGACAAATTGGTACGAGAACTCCTACTGTTGAATACATCTGCACCCAACAGAATATTTTGTTCATGTTATTGAAAGGGTATGAATCTCCAGAAATAGCTCTAAATTGTGGAATAATGTTAAGAGAATGCATCAGACATGAACCACTTGCAAAAATCATTTTGTGGTCGGAACAGTTTTATGATTTCTTCAGATATGTCGAAATGTCAACATTTGACATAGCTTCAGATGCATTTGCCACATTCAAGGATTTACTTACAAGACATAAATTGCTCAGTGCAGAATTTTTGGAACAGCATTATGATAGATTTTTCAGTGAATATGAGAAGTTACTTCATTCAGAAAATTATGTGACAAAAAGACAGTCACTGAAGCTTCTCGGTGAACTACTACTAGATAGACACAACTTCACAATTATGACAAAATACATCAGTAAACCTGAGAACCTCAAATTAATGATGAACCTGCTGCGAGACAAAAGTCGCAACATCCAGTTTGAGGCCTTTCACGTTTTTAAGGTGTTTGTAGCCAATCCTAACAAGACGCAGCCCATCCTAGACATCCTCCTCAAGAACCAGGCCAAACTCATAGAGTTCCTCAGCAAGTTTCAGAACGACAGGACGGAGGATGAGCAGTTTAACGACGAGAAGACCTATTTAGTTAAACAGATCAGGGATTTGAAGAGACCAGCTCAGCAAGAAGCTTAAGCATGCC

Amino Acid Sequence

LKB1
MAHHHHHHENLYFQGMEVVDPQQLGMFTEGELMSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSETLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHKNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEMLDSVPEKRFPVCQAHGYFCQLIDGLEYLHSQGIVHKDIKPGNLLLTTGGTLKISDLGVAEALHPFAADDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGKGSYAIPGDCGPPLSDLLKGMLEYEPAKRFSIRQIRQHSWFRKKHPPAEAPVPIPPSPDTKDRWRSMTVVPYLEDLHGADEDEDLFDIEDDIIYTQDFTVPGQVPEEEASHNGQRRGLPKAVCMNGTEAAQLSTKSRAEGRAPNPARKACSASSKIRRLSACKQQ

STRAD
MSFLVSKPERIRRWVSEKFIVEGLRDLELFGEQPPGDTRRKTNDASSESIASFSKQEVMSSFLPEGGCYELLTVIGKGFEDLMTVNLARYKPTGEYVTVRRINLEACSNEMVTFLQGELHVSKLFNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLICTHFMDGMNELAIAYILQGVLKALDYIHHMGYVHRSVKASHILISVDGKVYLSGLRSNLSMISHGQRQRVVHDFPKYSVKVLPWLSPEVLQQNLQGYDAKSDIYSVGITACELANGHVPFKDMPATQMLLEKLNGTVPCLLDTSTIPAEELTMSPSRSVANSGLSDSLTTSTPRPSNGDSPSHPYHRTFSPHFHHFVEQCLQRNPDARPSASTLLNHSFFKQIKRRASEALPELLRPVTPITNFEGSQSQDHSGIFGLVTNLEELEVDDWEF

MO25 ALPHA
MPFPFGKSHKSPADIVKNLKESMAVLEKQDISDKKAEKATEEVSKNLVAMKEILYGTNEKEPQTEAVAQLAQELYNSGLLSTLVADLQLIDFEGKKDVAQIFNNILRRQIGTRTPTVEYICTQQNILFMLLKGYESPEIALNCGIMLRECIRHEPLAKIILWSEQFYDFFRYVEMSTFDIASDAFATFKDLLTRHKLLSAEFLEQHYDRFFSEYEKLLHSENYVTKRQSLKLLGELLLDRHNFTIMTKYISKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQAKLIEFLSKFQNDRTEDEQFNDEKTYLVKQIRDLKRPAQQEA

• Vector Type: Baculovirus
• Full Snapgene Map: pMultiBac STRAD, MO25, His-LKB1200.05 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU13002
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Multibac vector from Imre Berger ETH Zurich.; See SnapGene file.
• Low Temp: No