cDNA Clone USP9X

• Plasmid: pGEX-6-PREC USP9X AA1553-end wt (2XPREC)
• Parent Plasmid: pGEX6P1
• Expressed: GST-PREC USP9X 1553-end wt
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCTGGAAGTTCTGTTCCAGGGCCCGAAAGGATTCGTGGGGCTGAAAAATGCCGGTGCTACTTGTTACATGAATTCTGTGATTCAGCAACTCTACATGATTCCTTCCATTAGGAACGGTATTCTTGCCATTGAAGGCACAGGTAGTGATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAATGTTGATCCCAGGGATGATGTATTTGGATATCCTCAACAATTTGAAGATAAACCAGCATTAAGTAAAACTGAAGGTAGAAAAGAGTACAACATTGGTGTCCTAAGACACCTTCAGGTCATCTTTGGTCATTTAGCTGCTTCTCGACTGCAATACTATGTGCCCAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGCCTGTTAATCTGCGTGAACAACACGATGCTTTAGAATTTTTTAATTCATTGGTGGATAGTTTAGATGAAGCTTTAAAAGCTTTAGGACATCCAGCTATGCTAAGTAAAGTCTTAGGAGGTTCCTTTGCTGATCAGAAGATCTGCCAAGGCTGCCCACATAGGTACGAATGTGAAGAATCTTTTACGACCCTAAACGTAGACATTAGAAATCACCAAAATCTTCTTGATTCTTTGGAACAGTATGTCAAAGGAGATTTACTAGAAGGTGCAAATGCATATCATTGTGAAAAATGCAATAAAAAGGTTGATACCGTAAAGCGCTTGCTGATTAAAAAATTACCTCCTGTTCTTGCTATACAACTAAAGCGATTTGACTATGACTGGGAAAGAGAATGTGCAATCAAGTTCAATGATTATTTTGAATTTCCTCGAGAGCTGGACATGGAACCTTACACAGTTGCAGGTGTCGCAAAGCTGGAAGGGGATAATGTAAACCCAGAGAGTCAGTTGATACAACAGAGTGAGCAGTCTGAAAGTGAGACAGCAGGAAGCACAAAATACAGACTTGTGGGTGTGCTCGTACACAGTGGTCAAGCGAGTGGGGGGCATTATTATTCTTACATCATCCAAAGGAATGGTGGAGATGGTGAGAGAAATCGCTGGTATAAATTTGATGATGGTGATGTAACAGAATGTAAAATGGATGATGACGAAGAAATGAAAAACCAGTGTTTTGGTGGAGAGTACATGGGAGAAGTGTTTGATCACATGATGAAGCGTATGTCATACAGGCGCCAGAAAAGGTGGTGGAATGCTTATATACTTTTTTATGAACGAATGGACACAATAGACCAAGATGATGAGTTGATAAGATATATATCAGAGCTTGCTATCACCACCAGACCTCATCAGATTATTATGCCATCAGCCATTGAGAGAAGTGTACGGAAACAGAACGTACAATTCATGCATAACCGAATGCAGTACAGTATGGAGTATTTTCAGTTTATGAAAAAACTGCTTACATGTAATGGCGTTTACTTAAACCCTCCTCCCGGGCAAGATCACCTGTTGCCTGAAGCAGAAGAAATCACTATGATCAGTATTCAACTTGCTGCTAGATTCCTCTTTACTACAGGATTTCACACAAAGAAAGTAGTCCGTGGCTCTGCCAGTGATTGGTATGATGCATTGTGTATTCTCCTTCGTCACAGCAAGAATGTACGTTTTTGGTTTGCTCATAACGTCCTTTTTAATGTTTCAAATCGCTTCTCCGAATACCTTCTGGAGTGCCCTAGTGCAGAAGTGAGGGGTGCGTTTGCAAAACTTATAGTCTTTATTGCACATTTTTCCTTGCAAGATGGGCCATGTCCTTCACCTTTTGCCTCTCCTGGACCTTCTAGTCAGGCTTATGACAACTTAAGCTTGAGTGATCACTTACTAAGAGCAGTACTAAATCTCTTGAGAAGGGAAGTTTCAGAGCATGGGCGTCATTTACAGCAGTATTTCAACCTGTTTGTAATGTATGCCAATTTAGGTGTGGCAGAGAAGACACAGCTTCTGAAATTGAGTGTACCTGCTACTTTTATGCTTGTGTCTTTAGATGAAGGTCCAGGTCCTCCAATCAAATACCAGTATGCTGAATTAGGCAAATTATACTCAGTAGTGTCACAGCTGATCCGCTGTTGCAATGTCTCTTCAAGAATGCAGTCTTCAATCAATGGTAATCCTCCTCTTCCCAATCCTTTTGGTGATCCTAATTTATCACAACCTATAATGCCAATTCAGCAGAATGTGGCAGACATTTTATTTGTGAGAACAAGTTATGTGAAGAAAATCATTGAAGACTGCAGTAATTCAGAGGAAACCGTCAAATTGCTTCGTTTTTGCTGCTGGGAGAATCCTCAGTTCTCATCTACTGTCCTCAGTGAACTTCTCTGGCAGGTTGCATATTCCTATACCTATGAACTGCGGCCCTATTTGGATCTGCTTTTGCAAATCTTACTGATTGGGGACTCCTGGCAAACTCACAGGATTCATAATGCACTGAAAGGAATTCCAGATGACCGAGATGGGCTGTTTGACACAATCCAGCGCTCTAAGAATCACTATCAAAAAAGAGCATACCAGTGTATAAAATGTATGGTAGCTCTATTTAGTAACTGTCCTGTTGCTTACCAAATCCTGCAGGGCAATGGAGATCTTAAAAGAAAGTGGACCTGGGCAGTGGAATGGCTTGGAGATGAACTTGAAAGAAGACCATATACTGGCAATCCTCAGTACACTTACAACAATTGGTCTCCCCCAGTGCAAAGCAATGAAACGTCCAATGGTTATTTCTTGGAGAGATCACATAGTGCTAGGATGACACTTGCAAAAGCTTGTGAACTCTGTCCAGAGGAGGAGCCAGATGACCAAGATGCTCCAGATGAACATGAGTCGCCTCCACCTGAAGATGCCCCATTGTACCCCCATTCACCTGGATCTCAGTATCAACAGAATAACCATGTGCATGGACAGCCATATACAGGCCCAGCAGCACATCACATGAACAACCCTCAGAGAACTGGCCAACGAGCACAAGAAAATTATGAAGGCAGTGAAGAAGTATCCCCACCTCAAACCAAGGATCAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSLEVLFQGPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQQFEDKPALSKTEGRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQSEQSESETAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGERNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDTIDQDDELIRYISELAITTRPHQIIMPSAIERSVRKQNVQFMHNRMQYSMEYFQFMKKLLTCNGVYLNPPPGQDHLLPEAEEITMISIQLAARFLFTTGFHTKKVVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPFASPGPSSQAYDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGVAEKTQLLKLSVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCNVSSRMQSSINGNPPLPNPFGDPNLSQPIMPIQQNVADILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIGDSWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCPVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHESPPPEDAPLYPHSPGSQYQQNNHVHGQPYTGPAAHHMNNPQRTGQRAQENYEGSEEVSPPQTKDQ*AAX*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU10959
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All USP9X plasmids MUST be grown at 30 degrees C or less to prevent recombination.