cDNA Clone USP9X

• Synonyms: DFFRX, FAF, FAM
• Plasmid: pGFP-TAP USP9X
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP USP9X wt
• Genbank: NM_001039591.2 GI:145309310 with silent changes: t856c, g6150a and a7062g. And coding changes a4904g (D1635G) and a7040g (E2347G); EST-derived.
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

GGATCCCCGGAATTCGCCCTTATGACAGCCACGACTCGTGGCTCTCCGGT
CGGAGGGAATGACAACCAGGGCCAGGCTCCTGATGGACAGTCTCAGCCCC
CCCTCCAACAGAATCAGACTTCATCGCCTGATTCTTCCAATGAAAATTCC
CCGGCAACTCCCCCAGATGAGCAAGGTCAAGGTGATGCCCCACCACAGCT
TGAAGATGAGGAACCTGCATTTCCACATACTGACTTGGCCAAGTTGGATG
ACATGATCAACAGGCCTCGATGGGTGGTTCCAGTTTTGCCGAAAGGGGAA
TTAGAAGTGCTTTTAGAAGCTGCTATTGATCTTAGTAAAAAGGGCCTTGA
TGTTAAAAGTGAAGCATGTCAGCGATTTTTCCGTGATGGGCTAACAATAT
CATTCACTAAAATTCTTACAGATGAAGCAGTGAGTGGCTGGAAGTTTGAA
ATTCATAGGTGTATTATTAACAATACTCATCGTCTGGTGGAGCTATGTGT
GGCCAAGTTGTCCCAAGACTGGTTTCCACTTTTAGAACTTCTTGCCATGG
CCTTAAATCCTCATTGCAAATTCCATATCTACAATGGTACACGTCCATGT
GAATCAGTTTCCTCAAGTGTTCAGTTGCCTGAAGATGAACTCTTTGCTCG
TTCTCCAGATCCTCGATCACCAAAGGGTTGGCTAGTGGATCTTCTCAACA
AATTTGGCACTTTAAATGGGTTCCAGATTTTGCATGATCGTTTTATTAAT
GGATCAGCATTAAACGTTCAAATAATTGCAGCCCTTATTAAACCATTTGG
GCAATGCTATGAGTTTCTCACTCTTCATACAGTGAAAAAGTACTTTCTTC
CAATAATAGAAATGGTTCCACAGTTTCTAGAAAACTTAACTGATGAAGAA
CTGAAAAAAGAAGCAAAGAATGAAGCCAAAAATGATGCTCTTTCAATGAT
TATTAAATCTTTGAAGAATTTAGCTTCAAGGGTTCCAGGACAAGAAGAAA
CTGTTAAAAACTTAGAAATATTTAGGTTAAAAATGATACTTAGATTATTG
CAAATTTCTTCTTTCAATGGAAAGATGAATGCACTGAATGAAGTTAATAA
GGTGATATCTAGTGTATCATACTATACTCATCGACATGGTAATCCTGAGG
AGGAAGAGTGGCTCACAGCTGAACGAATGGCAGAATGGATACAGCAGAAC
AATATCTTATCCATAGTGTTGCGAGATAGTCTTCATCAGCCACAGTATGT
AGAAAAGTTAGAGAAGATTCTTCGTTTTGTCATCAAAGAAAAAGCTCTGA
CCTTACAGGATCTTGATAATATCTGGGCAGCACAGGCAGGGAAACATGAA
GCCATTGTGAAGAATGTACATGATCTCCTGGCAAAATTGGCATGGGATTT
TTCTCCTGAACAACTTGATCATCTTTTTGATTGTTTTAAGGCCAGTTGGA
CAAATGCGAGTAAAAAGCAACGTGAAAAGCTACTTGAGCTGATACGTCGT
CTTGCAGAAGATGATAAAGATGGTGTGATGGCACACAAAGTGTTGAACCT
TCTGTGGAATCTGGCTCACAGTGATGATGTGCCTGTAGATATCATGGACC
TGGCTCTCAGTGCCCACATAAAAATACTAGATTACAGTTGCTCCCAGGAC
CGTGATACACAAAAGATCCAATGGATAGATCGCTTTATAGAAGAACTTCG
CACAAATGACAAATGGGTTATTCCCGCACTGAAACAAATTAGAGAAATTT
GTAGTTTGTTTGGTGAAGCGCCTCAAAATTTGAGTCAAACTCAGCGAAGT
CCCCATGTGTTTTATCGCCATGACTTAATCAATCAACTTCAACACAATCA
TGCCCTAGTTACTTTGGTAGCAGAAAACCTTGCAACTTACATGGAAAGCA
TGAGACTATATGCTAGAGACCATGAAGATTATGACCCACAAACTGTGAGG
CTGGGAAGTAGATATAGTCATGTTCAAGAAGTTCAAGAACGGCTTAACTT
CCTTAGATTTTTATTGAAGGATGGTCAGCTGTGGCTATGTGCTCCTCAGG
CAAAACAAATATGGAAATGCTTAGCTGAGAATGCAGTTTACCTTTGTGAT
CGTGAAGCCTGTTTTAAGTGGTATTCCAAGTTGATGGGGGATGAACCAGA
CTTAGATCCTGATATTAATAAGGACTTCTTTGAAAGTAATGTGCTTCAGC
TTGATCCTTCTCTGTTAACTGAAAATGGAATGAAGTGTTTTGAGCGATTC
TTCAAAGCTGTGAATTGTCGAGAAGGAAAACTAGTAGCAAAAAGGAGAGC
CTATATGATGGATGACTTGGAGTTAATAGGATTAGATTACCTTTGGAGGG
TCGTGATTCAGAGTAATGATGATATTGCCAGCAGAGCTATAGATCTCCTC
AAAGAGATATACACGAACCTTGGTCCAAGACTACAAGTCAATCAGGTGGT
GATCCATGAAGACTTCATTCAGTCTTGTTTTGATCGTCTGAAGGCTTCCT
ATGACACATTGTGTGTTTTGGATGGTGACAAAGACAGTGTTAATTGTGCA
AGACAGGAAGCTGTTCGAATGGTTCGAGTATTAACTGTTTTAAGGGAATA
TATAAATGAATGTGACAGTGATTATCATGAGGAAAGAACAATTCTCCCTA
TGTCGAGAGCATTCCGCGGTAAACACCTCTCTTTTGTAGTTCGATTTCCA
AACCAGGGCAGACAGGTTGATGACTTGGAGGTATGGTCTCATACAAATGA
TACAATTGGTTCAGTACGACGATGTATTCTCAATCGTATTAAAGCCAACG
TAGCCCATACAAAAATTGAGCTCTTTGTGGGCGGTGAGCTGATAGATCCT
GCAGATGATAGAAAGTTGATTGGACAATTAAACTTAAAAGATAAATCGCT
TATTACAGCCAAACTTACACAGATAAGTTCCAATATGCCTTCAAGCCCTG
ATAGCTCTTCTGATTCCTCCACTGGATCTCCTGGAAACCATGGTAATCAT
TACAGTGATGGTCCCAATCCAGAAGTGGAAAGCTGTTTGCCTGGAGTGAT
AATGTCACTGCATCCCAGATACATCTCTTTTCTTTGGCAAGTTGCAGACT
TAGGTAGCAGCCTAAATATGCCACCCCTTAGAGATGGAGCAAGAGTACTT
ATGAAACTTATGCCGCCAGATAGCACAACGATAGAAAAATTAAGAGCTAT
TTGTTTAGACCATGCCAAACTTGGAGAAAGCAGCCTTAGTCCATCTCTTG
ACTCACTTTTCTTTGGTCCTTCAGCCTCACAAGTGCTATATCTAACAGAG
GTAGTCTATGCCTTGTTAATGCCTGCTGGTGCACCTCTGGCTGATGATTC
CTCTGATTTTCAGTTTCACTTCTTGAAAAGTGGTGGCCTACCCCTTGTAC
TGAGTATGCTAACCAGAAATAACTTCCTACCGAATGCAGATATGGAAACT
CGAAGGGGTGCCTACCTCAATGCTCTTAAAATAGCCAAGCTTTTGCTAAC
TGCCATTGGCTATGGTCATGTTCGAGCTGTGGCAGAAGCTTGTCAGCCAG
GTGTAGAAGGTGTGAATCCCATGACACAGATCAACCAAGTTACCCATGAT
CAAGCAGTGGTGCTACAAAGTGCCCTTCAGAGCATTCCTAATCCATCATC
CGAGTGCATGCTTAGAAATGTGTCAGTTCGTCTTGCTCAGCAGATATCTG
ATGAGGCTTCAAGATATATGCCTGATATTTGTGTAATTAGAGCTATACAA
AAAATTATCTGGGCATCAGGATGTGGGTCGTTACAGCTAGTATTTAGCCC
AAATGAAGAAATCACTAAAATTTATGAGAAGACCAATGCAGGCAATGAGC
CAGACTTGGAAGACGAACAGGTTTGCTGTGAAGCATTGGAAGTGATGACC
TTATGTTTTGCCTTGATTCCAACAGCCTTAGATGCTCTTAGTAAAGAAAA
GGCTTGGCAGACATTCATCATTGACTTACTATTGCACTGTCACAGCAAAA
CTGTTCGTCAGGTGGCACAGGAGCAGTTCTTTTTAATGTGCACCAGATGT
TGCATGGGACACCGGCCTCTACTTTTCTTCATTACTCTACTCTTTACTGT
TTTGGGGAGCACAGCAAGAGAGAGAGCTAAACACTCAGGCGACTACTTTA
CTCTTTTAAGACACCTTCTTAATTACGCTTACAATAGTAATATTAATGTA
CCCAATGCTGAAGTTCTTCTCAATAATGAAATTGATTGGCTTAAAAGAAT
TAGGGATGATGTTAAAAGAACAGGAGAAACGGGTATTGAAGAGACGATCT
TAGAGGGCCACCTTGGAGTGACAAAGGAGTTACTGGCCTTTCAAACTTCT
GAGAAAAAATTTCATATTGGTTGTGAAAAAGGAGGTGCTAATCTCATTAA
AGAATTAATTGATGATTTCATATTTCCTGCATCCAATGTTTACCTACAGT
ATATGAGAAATGGAGAGCTTCCAGCTGAACAGGCTATTCCGGTCTGTGGT
TCACCACCTACAATTAATGCTGGTTTTGAATTACTTGTAGCATTAGCTGT
TGGCTGTGTGAGGAATCTCAAACAAATAGTAGATTCTTTGACTGAAATGT
ATTACATTGGCACAGCAATAACTACTTGTGAAGCACTTACTGAGTGGGAA
TATCTGCCACCTGTTGGACCCCGCCCACCCAAAGGATTCGTGGGGCTGAA
AAATGCCGGTGCTACTTGTTACATGAATTCTGTGATTCAGCAACTCTACA
TGATTCCTTCCATTAGGAACGGTATTCTTGCCATTGAAGGCACAGGTAGT
GATGTAGATGATGATATGTCTGGGGATGAGAAGCAGGACAATGAGAGCAA
TGTTGATCCCAGGGATGATGTATTTGGATATCCTCAACAATTTGAAGATA
AACCAGCATTAAGTAAAACTGAAGGTAGAAAAGAGTACAACATTGGTGTC
CTAAGACACCTTCAGGTCATCTTTGGTCATTTAGCTGCTTCTCGACTGCA
ATACTATGTGCCCAGAGGATTTTGGAAACAGTTCAGGCTTTGGGGTGAGC
CTGTTAATCTGCGTGAACAACACGATGCTTTAGAATTTTTTAATTCATTG
GTGGATAGTTTAGATGAAGCTTTAAAAGCTTTAGGACATCCAGCTATGCT
AAGTAAAGTCTTAGGAGGTTCCTTTGCTGATCAGAAGATCTGCCAAGGCT
GCCCACATAGGTACGAATGTGAAGAATCTTTTACGACCCTAAACGTAGAC
ATTAGAAATCACCAAAATCTTCTTGATTCTTTGGAACAGTATGTCAAAGG
AGATTTACTAGAAGGTGCAAATGCATATCATTGTGAAAAATGCAATAAAA
AGGTTGATACCGTAAAGCGCTTGCTGATTAAAAAATTACCTCCTGTTCTT
GCTATACAACTAAAGCGATTTGACTATGACTGGGAAAGAGAATGTGCAAT
CAAGTTCAATGATTATTTTGAATTTCCTCGAGAGCTGGACATGGAACCTT
ACACAGTTGCAGGTGTCGCAAAGCTGGAAGGGGATAATGTAAACCCAGAG
AGTCAGTTGATACAACAGAGTGAGCAGTCTGAAAGTGAGACAGCAGGAAG
CACAAAATACAGACTTGTGGGTGTGCTCGTACACAGTGGTCAAGCGAGTG
GGGGGCATTATTATTCTTACATCATCCAAAGGAATGGTGGAGATGGTGAG
AGAAATCGCTGGTATAAATTTGATGATGGTGATGTAACAGAATGTAAAAT
GGATGATGACGAAGAAATGAAAAACCAGTGTTTTGGTGGAGAGTACATGG
GAGAAGTGTTTGATCACATGATGAAGCGTATGTCATACAGGCGCCAGAAA
AGGTGGTGGAATGCTTATATACTTTTTTATGAACGAATGGACACAATAGA
CCAAGATGATGAGTTGATAAGATATATATCAGAGCTTGCTATCACCACCA
GACCTCATCAGATTATTATGCCATCAGCCATTGAGAGAAGTGTACGGAAA
CAGAACGTACAATTCATGCATAACCGAATGCAGTACAGTATGGAGTATTT
TCAGTTTATGAAAAAACTGCTTACATGTAATGGCGTTTACTTAAACCCTC
CTCCCGGGCAAGATCACCTGTTGCCTGAAGCAGAAGAAATCACTATGATC
AGTATTCAACTTGCTGCTAGATTCCTCTTTACTACAGGATTTCACACAAA
GAAAGTAGTCCGTGGCTCTGCCAGTGATTGGTATGATGCATTGTGTATTC
TCCTTCGTCACAGCAAGAATGTACGTTTTTGGTTTGCTCATAACGTCCTT
TTTAATGTTTCAAATCGCTTCTCCGAATACCTTCTGGAGTGCCCTAGTGC
AGAAGTGAGGGGTGCGTTTGCAAAACTTATAGTCTTTATTGCACATTTTT
CCTTGCAAGATGGGCCATGTCCTTCACCTTTTGCCTCTCCTGGACCTTCT
AGTCAGGCTTATGACAACTTAAGCTTGAGTGATCACTTACTAAGAGCAGT
ACTAAATCTCTTGAGAAGGGAAGTTTCAGAGCATGGGCGTCATTTACAGC
AGTATTTCAACCTGTTTGTAATGTATGCCAATTTAGGTGTGGCAGAGAAG
ACACAGCTTCTGAAATTGAGTGTACCTGCTACTTTTATGCTTGTGTCTTT
AGATGAAGGTCCAGGTCCTCCAATCAAATACCAGTATGCTGAATTAGGCA
AATTATACTCAGTAGTGTCACAGCTGATCCGCTGTTGCAATGTCTCTTCA
AGAATGCAGTCTTCAATCAATGGTAATCCTCCTCTTCCCAATCCTTTTGG
TGATCCTAATTTATCACAACCTATAATGCCAATTCAGCAGAATGTGGCAG
ACATTTTATTTGTGAGAACAAGTTATGTGAAGAAAATCATTGAAGACTGC
AGTAATTCAGAGGAAACCGTCAAATTGCTTCGTTTTTGCTGCTGGGAGAA
TCCTCAGTTCTCATCTACTGTCCTCAGTGAACTTCTCTGGCAGGTTGCAT
ATTCCTATACCTATGAACTGCGGCCCTATTTGGATCTGCTTTTGCAAATC
TTACTGATTGGGGACTCCTGGCAAACTCACAGGATTCATAATGCACTGAA
AGGAATTCCAGATGACCGAGATGGGCTGTTTGACACAATCCAGCGCTCTA
AGAATCACTATCAAAAAAGAGCATACCAGTGTATAAAATGTATGGTAGCT
CTATTTAGTAACTGTCCTGTTGCTTACCAAATCCTGCAGGGCAATGGAGA
TCTTAAAAGAAAGTGGACCTGGGCAGTGGAATGGCTTGGAGATGAACTTG
AAAGAAGACCATATACTGGCAATCCTCAGTACACTTACAACAATTGGTCT
CCCCCAGTGCAAAGCAATGAAACGTCCAATGGTTATTTCTTGGAGAGATC
ACATAGTGCTAGGATGACACTTGCAAAAGCTTGTGAACTCTGTCCAGAGG
AGGAGCCAGATGACCAAGATGCTCCAGATGAACATGAGTCGCCTCCACCT
GAAGATGCCCCATTGTACCCCCATTCACCTGGATCTCAGTATCAACAGAA
TAACCATGTGCATGGACAGCCATATACAGGCCCAGCAGCACATCACATGA
ACAACCCTCAGAGAACTGGCCAACGAGCACAAGAAAATTATGAAGGCAGT
GAAGAAGTATCCCCACCTCAAACCAAGGATCAATGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSPEF
ALMTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQTSSPDSSNENSPATPP
DEQGQGDAPPQLEDEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLL
EAAIDLSKKGLDVKSEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCI
INNTHRLVELCVAKLSQDWFPLLELLAMALNPHCKFHIYNGTRPCESVSS
SVQLPEDELFARSPDPRSPKGWLVDLLNKFGTLNGFQILHDRFINGSALN
VQIIAALIKPFGQCYEFLTLHTVKKYFLPIIEMVPQFLENLTDEELKKEA
KNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSF
NGKMNALNEVNKVISSVSYYTHRHGNPEEEEWLTAERMAEWIQQNNILSI
VLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAAQAGKHEAIVKN
VHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDD
KDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQK
IQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFY
RHDLINQLQHNHALVTLVAENLATYMESMRLYARDHEDYDPQTVRLGSRY
SHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACF
KWYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVN
CREGKLVAKRRAYMMDDLELIGLDYLWRVVIQSNDDIASRAIDLLKEIYT
NLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVLDGDKDSVNCARQEAV
RMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFVVRFPNQGRQ
VDDLEVWSHTNDTIGSVRRCILNRIKANVAHTKIELFVGGELIDPADDRK
LIGQLNLKDKSLITAKLTQISSNMPSSPDSSSDSSTGSPGNHGNHYSDGP
NPEVESCLPGVIMSLHPRYISFLWQVADLGSSLNMPPLRDGARVLMKLMP
PDSTTIEKLRAICLDHAKLGESSLSPSLDSLFFGPSASQVLYLTEVVYAL
LMPAGAPLADDSSDFQFHFLKSGGLPLVLSMLTRNNFLPNADMETRRGAY
LNALKIAKLLLTAIGYGHVRAVAEACQPGVEGVNPMTQINQVTHDQAVVL
QSALQSIPNPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQKIIWA
SGCGSLQLVFSPNEEITKIYEKTNAGNEPDLEDEQVCCEALEVMTLCFAL
IPTALDALSKEKAWQTFIIDLLLHCHSKTVRQVAQEQFFLMCTRCCMGHR
PLLFFITLLFTVLGSTARERAKHSGDYFTLLRHLLNYAYNSNINVPNAEV
LLNNEIDWLKRIRDDVKRTGETGIEETILEGHLGVTKELLAFQTSEKKFH
IGCEKGGANLIKELIDDFIFPASNVYLQYMRNGELPAEQAIPVCGSPPTI
NAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPV
GPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNGILAIEGTGSDVDDD
MSGDEKQDNESNVDPRDDVFGYPQQFEDKPALSKTEGRKEYNIGVLRHLQ
VIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLD
EALKALGHPAMLSKVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQ
NLLDSLEQYVKGDLLEGANAYHCEKCNKKVDTVKRLLIKKLPPVLAIQLK
RFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQ
QSEQSESETAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGERNRWY
KFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNA
YILFYERMDTIDQDDELIRYISELAITTRPHQIIMPSAIERSVRKQNVQF
MHNRMQYSMEYFQFMKKLLTCNGVYLNPPPGQDHLLPEAEEITMISIQLA
ARFLFTTGFHTKKVVRGSASDWYDALCILLRHSKNVRFWFAHNVLFNVSN
RFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSPFASPGPSSQAYD
NLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGVAEKTQLLK
LSVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCNVSSRMQSS
INGNPPLPNPFGDPNLSQPIMPIQQNVADILFVRTSYVKKIIEDCSNSEE
TVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIGD
SWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNC
PVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQS
NETSNGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHESPPPEDAPL
YPHSPGSQYQQNNHVHGQPYTGPAAHHMNNPQRTGQRAQENYEGSEEVSP
PQTKDQ*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU10786
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Treat carefully. Grow at low temp. Can be difficult to propagate.; ; All USP9X plasmids MUST be grown at 30 degrees C or less to prevent recombination.