cDNA Clone WNK1

• Plasmid: GFP WNK1 1900-end
• Parent Plasmid: pEGFPC1
• Expressed: GFP-WNK1 1900-end
• Tag: GFP
• Species: Human

Sequence of Insert

CCAGAGAGTACCTTGGTGAAACCAGAGCCGAATGGCATAACCATCCCTGGTATCTCTTCAGATGTGCCAGAGAGTGCCCACAAAACTACTGCCTCAGAGG
CAAAGTCAGACACTGGGCAGCCTACCAAGGTTGGACGTTTTCAGGTGACAACTACAGCAAACAAAGTGGGTCGTTTCTCTGTATCAAAAACTGAGGACAA
GATCACTGACACAAAGAAAGAAGGACCAGTGGCATCTCCTCCTTTTATGGATTTGGAACAAGCTGTTCTTCCTGCTGTGATACCAAAGAAAGAGAAGCCT
GAACTGTCAGAGCCTTCACATCTAAATGGGCCGTCTTCTGACCCGGAGGCCGCTTTTTTAAGTAGGGATGTGGATGATGGTTCCGGTAGTCCACACTCGC
CCCATCAGCTGAGCTCAAAGAGCCTTCCTAGCCAGAATCTAAGTCAAAGCCTTAGTAATTCATTTAACTCCTCTTACATGAGTAGCGACAATGAGTCAGA
TATCGAAGATGAAGACTTAAAGTTAGAGCTGCGACGACTACGAGATAAACATCTCAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAA
TCTTTGTATACCAAACTGGGCAAGGTGCCCCCTGCTGTTATTATTCCCCCAGCTGCTCCCCTTTCAGGGAGAAGACGACGACCCACTAAAAGCAAAGGCA
GCAAATCTAGTCGAAGCAGTTCCTTGGGGAATAAAAGCCCCCAGCTTTCAGGTAACCTGTCTGGTCAGAGTGCAGCTTCAGTCTTGCACCCCCAGCAGAC
CCTCCACCCTCCTGGCAACATCCCAGAGTCCGGGCAGAATCAGCTGTTACAGCCCCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTCACC
AGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGAACCAGCAGCACAAACACTGTTGGGGCAACAGTGAACAGCCAAGCCGCCCAAG
CTCAGCCTCCTGCCATGACGTCCAGCAGGAAGGGCACATTCACAGATGACTTGCACAAGTTGGTAGACAATTGGGCCCGAGATGCCATGAATCTCTCAGG
CAGGAGAGGAAGCAAAGGGCACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCACCTGGGCAACTGTGCATCTCCATGACCTCGAACCTG
GGTGGCTCTGCCCCCATCTCTGCAGCATCAGCTACCTCTCTAGGTCACTTCACCAAGTCTATGTGCCCCCCACAGCAGTATGGCTTTCCAGCTACCCCAT
TTGGCGCTCAATGGAGTGGGACGGGTGGCCCAGCACCACAGCCACTTGGCCAGTTCCAACCTGTGGGAACTGCCTCCTTGCAGAATTTCAACATCAGCAA
TTTGCAGAAATCCATCAGCAACCCCCCAGGCTCCAACCTGCGGACCACTTAGATCGATGTCGACAAGGGCGAATTCTGCAGATATCCATCACACTGGCGG
CCGCAAGGGCGAATTCTGCAGTCGACGGTACCGCGGGCCCGGGATCCACCGGATCTAGATAACTGATCATAATCAGCCATACCACATTTGTAGAGGTTTT
ACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAATACATAAATATGAACTTGG

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSV
SKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMS
SDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASV
LHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARD
AMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQ
NFNISNLQKSISNPPGSNLRTT*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU10428
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All WNK plasmids MUST be grown at 30 degrees C or less to prevent recombination.