cDNA Clone NKCC1 (shark)

• Plasmid: pCMV-FLAG NKCC (shark) wt
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG-NKCC (shark) wt
• Genbank: A53491
• Tag: FLAG
• Species: Shark

Sequence of Insert

GCGGCCGCCATGGAGCCCGCCTTTCCCGCTTCCTCGGCCGGCGTGCAGTCCCAGTCCGGCCCGGAGCCTGGTGCCGGCCAACAGGAGCCGCCGCCGCCGGCCACGCCGCTCCGTCCCGTAGCGAGTCAGAGCCGCTTTCAGGTCGACCTGGTGACCGAGGGTGGAGGTGGTGGAGACGGGCAGAAGGGACAGACCGCAGCCCAGCCTGCCGCCGCCGCCAAGGACAAGGACAGGGGGGACGGCGGTGCAGCGGCTCCGTCTCCAGCCTCACCTGCCGCCGCCGCCGAGCCGCCCGCCGCAGCAGCAGAAGAGGCGAAAGGCAGATTCCGGGTGAATTTCGTCGACCCGGCTTCGGATGAGCCGCCGCTGTCCAGCCAGCAGCAGCCGCCGCCGCCCAGCTCGGCATCGTCGGCGCACGGCGGCCACCAGCCGCCCAGCGAGAGCATGAACGGCTACCCGCAGAACGGCGATACCATGATGAGCGAGGGCAGCCTGCACTCGTCCGGCACCGGGGCTCACCATTACTACGATACGCACACCAACACTTACTATCTCAGGACTTTCGGCCACAACACCATCGACGCGGTGCCCAGGATCGATCATTACAGGCACACGGTGGCCCAACTGGGCGAGAAGCTCATCAGACCCAGCCTGGCTGAGCTACACGATGAGCTCGACAAGGAGCCTTTTGAGGATGGCTATGTAAATGGCGAAGAAAGCAGTCCAGCTGAAGAGGCTGTGTCGAAGCATGTTGCTGACAATAAAGGTGTTGTAAAGTTTGGCTGGATTAAAGGTGTTCTGGTACGATGTATGCTAAATATCTGGGGTGTGATGCTCTTCATCCGTTTGTCATGGATTGTTGGCCACGCTGGGATAGGTCTTGCTCTTCTAGTCATTGGCACGGCGACTGTTGTGACCACTATCACAGGACTATCGACGTCTGCTATAACTACCAATGGATTTGTTCGTGGAGGTGGAGCATATTACTTAATATCCAGAAGTTTGGGACCTGAATTTGGTGGTGCAATTGGTCTCATTTTTGCCTTTGCCAATGCAGTAGCTGTAGCCATGTATGTGGTTGGCTTCGCGGAGACGGTTCGAGATCTGCTTGTGGAACATAATGCACTCATGATTGATGAAATGAGTGACATCCGGATTATAGGATCCGTTACTATCGTGGTCCTGTTTGGCATATCTGTCGCAGGGATGGAATGGGAAGCTAAGGCTCAAATTGTGCTGTTGGGAATTCTGCTATTAGCTATAGTTAACTTCACTGTGGGAACTTTCATCCCAGCCAATGACAAACGAGCGAAAGGATTTTTTAATTACCGAGGTGAAATATTTTCTGAAAACTTTGTGCCGGATTTTCGTGATGGAGAAGATTTCTTCTCGGTCTTTGCTATTTTCTTCCCTGCTGCTACTGGTATCCTTGCTGGGGCAAACATCTCCGGTGATCTTGCAGATCCACAATTAGCCATACCTAAAGGCACACTCTTGGCTATTTTAATCACCACAATAGTCTATGCAGGAGCGGCTGTTTCTGTAGGTTCTTGTATTGTACGTGAAGCTACTGGGAACCTTACTGATGCTATCATCCCTGGAACAGTGACTAACTGCACTAATGTTGCCTGTAAATTGGGCTTCAATTTCTCCTCTTGTGCCACAAATAAATGCTCCTATGGTTTAATGAATGACTTCCAGGTCATGAGCCTGGTGTCTGGATTTGGACCTTTGATTACTGCAGGAATCTTTTCAGCTACACTATCTTCTGCCTTGGCCTCGTTAGTGAGTGCTCCCAAAATATTCCAGGCTTTGTGTAAGGACAATATATATCCTGGTCTTCACGTTTTTTCAGTTGGTTATGGCAAGAACAATGAACCATTGCGTGGATATGTTCTCACATTCTTCATTGGTCTGGGATTCATATTAATTGCTGAGCTGAATGTCATTGCGCCAATCATTTCCAATTTTTTCCTGGCTTCATACGCTTTGATTAATTTCTCAGTGTTCCATGCTTCTCTTGCTAAATCTCCAGGCTGGCGACCTGCATTTAGATTCTATAACATGTGGATTTCATTGATTGGTGCTATCTTGTGCTGTGGAGTAATGTTTGTCATCAATTGGTGGGCAGCACTATTGACAAACGTCATTGTCCTTGCTTTATATATCTACGTCACCTACAAGAAACCAGATGTTAATTGGGGCTCCTCCACACAGGCTTTGACTTATTTGAATGCCTTGCAGCATGCCATTCGTCTTACAGGAGTAGAGGACCACGTGAAGAATTTCAGGCCCCAATGCCTATTAATGACAGGTGCTCCTACATCCCGTCCAGCTTTGCTCCATCTTGTTCATGCCTTCACCAAAAATGTTGGACTAGTGGTCTGTGGACATGTTCATACGGGGCCTCGCAGACAAGCTTTGAAGGAAATCTCCACTGATCAGGCTAAATATCAACGGTGGTTAATCAAAAACAAAATGAAAGCCTTCTATGCGCCTGTATATGCAGAAGATCTGCGTGAGGGAACACAGTTCTTGCTGCAGGCTGTAGGACTTGGTCGAATGCGGCCCAACACACTTGTCTTTGGCTTTAAAAAAGACTGGCGCCAGGCCCTTATGAAGGATGTGGAAAATTACATCAATGCTATTCATGATGCATTTGACTATCAGTATGGAGTAGTCGTCATTAGACTAAAGGAAGGTTTTAATATTTCCCATCTTCAAGCACAAGAAGAGCTGTGCACTTCTCAGGAGAAATCTGCTCATCCCAAGGACATAGTGGTGAATCTGGAACATTCTGATGCAGACTCTTCCAAGCCATCTTCTAAGTCAGTGAGTGAAACCAACAGCCCAGCAGTGTGTCAAGACCAAAAAGATGAAGAAAATGATGGCAAGGCGTCAACACAGCCACTCTTGAAAAAAGAGGTTAAAGATCCATCCGTGCCTTTGAACATGACTGACCAGAAGCTGCTTCAGGCCAGCTCTCAGTTCCAGAAGAAACAGGGCAAAGGGACTATTGACGTCTGGTGGCTTTTCGATGACGGAGGTCTGACGCTACTTATCCCGTATCTTCTCACTACCAAGAAGAAGTGGAAAGATTGCAAGATCAGAGTGTTCATCGGTGGAAAAATAAACAGGATAGATCACGACAGGAGGACAATGGCCACTTTACTCAGCAAATTCAGGATAGATTTTTCAGACATCACCGTTCTCGGGGATATGAACACTAAGCCAAGCAAAGACAATATTACAGCTTTTGAAGAAATGATTGAACCATTCAGACTTCACGAGGATGACAAAGAACAAGAAGCATCTGAGAAAATGAAGGAGGAGGAGCCATGGAGGATCACGGATAATGAGCTTGAGATCTACAGAATGAAGACATATCGACAGATCAGATTGAATGAATTGCTGCGGGAGAATTCAGGCACTGCTAACCTCATTGTCATGAGTCTGCCAGTGGCACGGAAAGGCGCTGTATCCAGTGCGTTGTATATGGCCTGGATTGAAACGCTCTCGAAGGATTTGCCACCCATCTTACTCGTGCGAGGCAACCACCAGAGTGTTCTTACTTTCTACTCCTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMEPAFPASSAGVQSQSGPEPGAGQQEPPPPATPLRPVASQSRFQVDLVTEGGGGGDGQKGQTAAQPAAAAKDKDRGDGGAAAPSPASPAAAAEPPAAAAEEAKGRFRVNFVDPASDEPPLSSQQQPPPPSSASSAHGGHQPPSESMNGYPQNGDTMMSEGSLHSSGTGAHHYYDTHTNTYYLRTFGHNTIDAVPRIDHYRHTVAQLGEKLIRPSLAELHDELDKEPFEDGYVNGEESSPAEEAVSKHVADNKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGHAGIGLALLVIGTATVVTTITGLSTSAITTNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVRDLLVEHNALMIDEMSDIRIIGSVTIVVLFGISVAGMEWEAKAQIVLLGILLLAIVNFTVGTFIPANDKRAKGFFNYRGEIFSENFVPDFRDGEDFFSVFAIFFPAATGILAGANISGDLADPQLAIPKGTLLAILITTIVYAGAAVSVGSCIVREATGNLTDAIIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHVHTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKDVENYINAIHDAFDYQYGVVVIRLKEGFNISHLQAQEELCTSQEKSAHPKDIVVNLEHSDADSSKPSSKSVSETNSPAVCQDQKDEENDGKASTQPLLKKEVKDPSVPLNMTDQKLLQASSQFQKKQGKGTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMSLPVARKGAVSSALYMAWIETLSKDLPPILLVRGNHQSVLTFYS*AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU6993
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee