cDNA Clone NKCC1 (shark)

• Plasmid: TOPO-STRAT-NKCC1 (shark)
• Parent Plasmid: pSC-B
• Genbank: A53491
• Species: Shark

Sequence of Insert

GCGGCCGCCATGGAGCCCGCCTTTCCCGCTTCCTCGGCCGGCGTGCAGTCCCAGTCCGGCCCGGAGCCTGGTGCCGGCCAACAGGAGCCGCCGCCGCCGGCCACGCCGCTCCGTCCCGTAGCGAGTCAGAGCCGCTTTCAGGTCGACCTGGTGACCGAGGGTGGAGGTGGTGGAGACGGGCAGAAGGGACAGACCGCAGCCCAGCCTGCCGCCGCCGCCAAGGACAAGGACAGGGGGGACGGCGGTGCAGCGGCTCCGTCTCCAGCCTCACCTGCCGCCGCCGCCGAGCCGCCCGCCGCAGCAGCAGAAGAGGCGAAAGGCAGATTCCGGGTGAATTTCGTCGACCCGGCTTCGGATGAGCCGCCGCTGTCCAGCCAGCAGCAGCCGCCGCCGCCCAGCTCGGCATCGTCGGCGCACGGCGGCCACCAGCCGCCCAGCGAGAGCATGAACGGCTACCCGCAGAACGGCGATACCATGATGAGCGAGGGCAGCCTGCACTCGTCCGGCACCGGGGCTCACCATTACTACGATACGCACACCAACACTTACTATCTCAGGACTTTCGGCCACAACACCATCGACGCGGTGCCCAGGATCGATCATTACAGGCACACGGTGGCCCAACTGGGCGAGAAGCTCATCAGACCCAGCCTGGCTGAGCTACACGATGAGCTCGACAAGGAGCCTTTTGAGGATGGCTATGTAAATGGCGAAGAAAGCAGTCCAGCTGAAGAGGCTGTGTCGAAGCATGTTGCTGACAATAAAGGTGTTGTAAAGTTTGGCTGGATTAAAGGTGTTCTGGTACGATGTATGCTAAATATCTGGGGTGTGATGCTCTTCATCCGTTTGTCATGGATTGTTGGCCACGCTGGGATAGGTCTTGCTCTTCTAGTCATTGGCACGGCGACTGTTGTGACCACTATCACAGGACTATCGACGTCTGCTATAACTACCAATGGATTTGTTCGTGGAGGTGGAGCATATTACTTAATATCCAGAAGTTTGGGACCTGAATTTGGTGGTGCAATTGGTCTCATTTTTGCCTTTGCCAATGCAGTAGCTGTAGCCATGTATGTGGTTGGCTTCGCGGAGACGGTTCGAGATCTGCTTGTGGAACATAATGCACTCATGATTGATGAAATGAGTGACATCCGGATTATAGGATCCGTTACTATCGTGGTCCTGTTTGGCATATCTGTCGCAGGGATGGAATGGGAAGCTAAGGCTCAAATTGTGCTGTTGGGAATTCTGCTATTAGCTATAGTTAACTTCACTGTGGGAACTTTCATCCCAGCCAATGACAAACGAGCGAAAGGATTTTTTAATTACCGAGGTGAAATATTTTCTGAAAACTTTGTGCCGGATTTTCGTGATGGAGAAGATTTCTTCTCGGTCTTTGCTATTTTCTTCCCTGCTGCTACTGGTATCCTTGCTGGGGCAAACATCTCCGGTGATCTTGCAGATCCACAATTAGCCATACCTAAAGGCACACTCTTGGCTATTTTAATCACCACAATAGTCTATGCAGGAGCGGCTGTTTCTGTAGGTTCTTGTATTGTACGTGAAGCTACTGGGAACCTTACTGATGCTATCATCCCTGGAACAGTGACTAACTGCACTAATGTTGCCTGTAAATTGGGCTTCAATTTCTCCTCTTGTGCCACAAATAAATGCTCCTATGGTTTAATGAATGACTTCCAGGTCATGAGCCTGGTGTCTGGATTTGGACCTTTGATTACTGCAGGAATCTTTTCAGCTACACTATCTTCTGCCTTGGCCTCGTTAGTGAGTGCTCCCAAAATATTCCAGGCTTTGTGTAAGGACAATATATATCCTGGTCTTCACGTTTTTTCAGTTGGTTATGGCAAGAACAATGAACCATTGCGTGGATATGTTCTCACATTCTTCATTGGTCTGGGATTCATATTAATTGCTGAGCTGAATGTCATTGCGCCAATCATTTCCAATTTTTTCCTGGCTTCATACGCTTTGATTAATTTCTCAGTGTTCCATGCTTCTCTTGCTAAATCTCCAGGCTGGCGACCTGCATTTAGATTCTATAACATGTGGATTTCATTGATTGGTGCTATCTTGTGCTGTGGAGTAATGTTTGTCATCAATTGGTGGGCAGCACTATTGACAAACGTCATTGTCCTTGCTTTATATATCTACGTCACCTACAAGAAACCAGATGTTAATTGGGGCTCCTCCACACAGGCTTTGACTTATTTGAATGCCTTGCAGCATGCCATTCGTCTTACAGGAGTAGAGGACCACGTGAAGAATTTCAGGCCCCAATGCCTATTAATGACAGGTGCTCCTACATCCCGTCCAGCTTTGCTCCATCTTGTTCATGCCTTCACCAAAAATGTTGGACTAGTGGTCTGTGGACATGTTCATACGGGGCCTCGCAGACAAGCTTTGAAGGAAATCTCCACTGATCAGGCTAAATATCAACGGTGGTTAATCAAAAACAAAATGAAAGCCTTCTATGCGCCTGTATATGCAGAAGATCTGCGTGAGGGAACACAGTTCTTGCTGCAGGCTGTAGGACTTGGTCGAATGCGGCCCAACACACTTGTCTTTGGCTTTAAAAAAGACTGGCGCCAGGCCCTTATGAAGGATGTGGAAAATTACATCAATGCTATTCATGATGCATTTGACTATCAGTATGGAGTAGTCGTCATTAGACTAAAGGAAGGTTTTAATATTTCCCATCTTCAAGCACAAGAAGAGCTGTGCACTTCTCAGGAGAAATCTGCTCATCCCAAGGACATAGTGGTGAATCTGGAACATTCTGATGCAGACTCTTCCAAGCCATCTTCTAAGTCAGTGAGTGAAACCAACAGCCCAGCAGTGTGTCAAGACCAAAAAGATGAAGAAAATGATGGCAAGGCGTCAACACAGCCACTCTTGAAAAAAGAGGTTAAAGATCCATCCGTGCCTTTGAACATGACTGACCAGAAGCTGCTTCAGGCCAGCTCTCAGTTCCAGAAGAAACAGGGCAAAGGGACTATTGACGTCTGGTGGCTTTTCGATGACGGAGGTCTGACGCTACTTATCCCGTATCTTCTCACTACCAAGAAGAAGTGGAAAGATTGCAAGATCAGAGTGTTCATCGGTGGAAAAATAAACAGGATAGATCACGACAGGAGGACAATGGCCACTTTACTCAGCAAATTCAGGATAGATTTTTCAGACATCACCGTTCTCGGGGATATGAACACTAAGCCAAGCAAAGACAATATTACAGCTTTTGAAGAAATGATTGAACCATTCAGACTTCACGAGGATGACAAAGAACAAGAAGCATCTGAGAAAATGAAGGAGGAGGAGCCATGGAGGATCACGGATAATGAGCTTGAGATCTACAGAATGAAGACATATCGACAGATCAGATTGAATGAATTGCTGCGGGAGAATTCAGGCACTGCTAACCTCATTGTCATGAGTCTGCCAGTGGCACGGAAAGGCGCTGTATCCAGTGCGTTGTATATGGCCTGGATTGAAACGCTCTCGAAGGATTTGCCACCCATCTTACTCGTGCGAGGCAACCACCAGAGTGTTCTTACTTTCTACTCCTAAGCGGCCGC

Amino Acid Sequence

AAAMEPAFPASSAGVQSQSGPEPGAGQQEPPPPATPLRPVASQSRFQVDLVTEGGGGGDGQKGQTAAQPAAAAKDKDRGDGGAAAPSPASPAAAAEPPAAAAEEAKGRFRVNFVDPASDEPPLSSQQQPPPPSSASSAHGGHQPPSESMNGYPQNGDTMMSEGSLHSSGTGAHHYYDTHTNTYYLRTFGHNTIDAVPRIDHYRHTVAQLGEKLIRPSLAELHDELDKEPFEDGYVNGEESSPAEEAVSKHVADNKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGHAGIGLALLVIGTATVVTTITGLSTSAITTNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVRDLLVEHNALMIDEMSDIRIIGSVTIVVLFGISVAGMEWEAKAQIVLLGILLLAIVNFTVGTFIPANDKRAKGFFNYRGEIFSENFVPDFRDGEDFFSVFAIFFPAATGILAGANISGDLADPQLAIPKGTLLAILITTIVYAGAAVSVGSCIVREATGNLTDAIIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHVHTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKDVENYINAIHDAFDYQYGVVVIRLKEGFNISHLQAQEELCTSQEKSAHPKDIVVNLEHSDADSSKPSSKSVSETNSPAVCQDQKDEENDGKASTQPLLKKEVKDPSVPLNMTDQKLLQASSQFQKKQGKGTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMSLPVARKGAVSSALYMAWIETLSKDLPPILLVRGNHQSVLTFYS*AA

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU6975
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee